##################################
# NO CUT ENZYMES (  211 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AfaI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaI        
AvaII       AviII       AvrII       BalI        BamHI       
BanI        BanII       BbeI        BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BfaI        
BfrI        BglI        BglII       BlnI        BmyI        
BpmI        BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BseRI       BsiEI       
BsiHKAI     BsiWI       BsmBI       BsmFI       BsoBI       
Bsp1286I    Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstF5I      BstPI       BstUI       
BstXI       BstZ17I     Bsu36I      Cac8I       CfoI        
Cfr10I      Cfr13I      ClaI        CpoI        DraI        
DraII       DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT14I     EcoT22I     FauI        FbaI        
FokI        FseI        FspI        HaeII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HinfI       HpaI        KasI        KpnI        
KspI        MaeI        MaeII       MaeIII      MamI        
MfeI        MluI        MluNI       MroI        MscI        
MslI        MspA1I      MunI        MvnI        MwoI        
NaeI        NarI        NcoI        NdeI        NgoMIV      
NheI        NlaIII      NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsaI        RsrII       
SacI        SacII       SalI        Sau96I      SbfI        
ScaI        SexAI       SfaNI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        TaqI        TfiI        
Tsp45I      Tsp509I     TspRI       Tth111I     TthHB8I     
XbaI        XcmI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AlwI          121  GATCC                GGATC(4/5)
BbvI           39  GCAGC                GCAGC(8/12)
BcnI          124  CCCGG                CC^[GC]GG
BlpI            9  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I        9  GCTGAGC              GC^T[ACGT]AGC
BsaJI          99  CCTGGG               C^C[ACGT][ACGT]GG
BsgI          116  GTGCAG               GTGCAG(16/14)
BsmAI          30  GAGAC                GTCTC(1/5)
BsmI          128  GAATGC               GAATGC(1/-1)
BsrI           57  CCAGT                ACTGG(1/-1)
BstNI          99  CCTGG                CC^[AT]GG
BstYI         120  AGATCC               [GA]^GATC[CT]
CelII           9  GCTGAGC              GC^T[ACGT]AGC
DdeI           10  CTGAG                C^T[ACGT]AG
DpnII         121  GATC                 ^GATC
EarI           43  CTCTTC               CTCTTC(1/4)
EcoRII         99  CCTGG                ^CC[AT]GG
Fnu4HI         39  GCAGC                GC^[ACGT]GC
HaeIII         21  GGCC                 GG^CC
HapII         125  CCGG                 C^CGG
HpaII         125  CCGG                 C^CGG
HphI            3  GGTGA                GGTGA(8/7)
ItaI           39  GCAGC                GC^[ACGT]GC
Ksp632I        43  CTCTTC               CTCTTC(1/4)
MboI          121  GATC                 ^GATC
MboII          44  TCTTC                GAAGA(8/7)
MflI          120  AGATCC               [GA]^GATC[CT]
MseI          107  TTAA                 T^TAA
MspI          125  CCGG                 C^CGG
MvaI           99  CCTGG                CC^[AT]GG
NciI          124  CCCGG                CC^[GC]GG
NdeII         121  GATC                 ^GATC
NlaIV          53  GGAGCC               GG[ACGT]^[ACGT]CC
PflMI          65  CCATTCGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SapI           42  GCTCTTC              GCTCTTC(1/4)
Sau3AI        121  GATC                 ^GATC
SfcI           34  CTACAG               C^T[GA][CT]AG
Tru9I         107  TTAA                 T^TAA
TseI           39  GCAGC                G^C[AT]GC
Van91I         65  CCATTCGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         120  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (    5 enzymes) #
###################################

AluI            8  AGCT                 AG^CT
               41  AGCT                
BsiYI          65  CCATTCGCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              153  CCTACTTTGGG         
BslI           65  CCATTCGCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              153  CCTACTTTGGG         
BssKI          99  CCTGG                ^CC[ACGT]GG
              124  CCCGG               
ScrFI          99  CCTGG                CC^[ACGT]GG
              124  CCCGG               


#########################################
# 3 OR MORE CUT ENZYMES (    1 enzymes) #
#########################################

MnlI