Restriction map for
pFN21AE2447
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# NO CUT ENZYMES ( 127 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AlwNI
AocI Aor51HI AscI AseI AsnI
Asp718I AspI AviII AvrII BalI
BanI BbeI BbrPI BbsI BbvCI
BcgI BciVI BcnI BfrI BglI
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaHI BsaI BseAI BsiEI BsiWI
BspDI BspEI BspLU11I BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstUI
BstXI Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraIII DrdI DsaI
EagI EclXI Eco47III Eco52I Eco81I
EcoRV EcoT22I FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HpaI
KasI KpnI KspI MluI MluNI
MroI MscI MvnI NaeI NarI
NciI NcoI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PstI PvuI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SphI Sse8387I StuI
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AhdI 2425 GACCTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 798 GTGCAC G^TGCAC
ApaI 611 GGGCCC GGGCC^C
ApaLI 798 GTGCAC G^TGCAC
Asp700I 1390 GAAGTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2425 GACCTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1741 CTCGGG C^[CT]CG[GA]G
BamHI 894 GGATCC G^GATCC
BclI 2043 TGATCA T^GATCA
BglII 406 AGATCT A^GATCT
BsaWI 346 TCCGGT [AT]^CCGG[AT]
BseRI 1738 CTCCTC GAGGAG(10/8)
BsgI 1046 CTGCAC GTGCAG(16/14)
BsmBI 2194 CGTCTC CGTCTC(1/5)
BsoBI 1741 CTCGGG C^[CT]CG[GA]G
Bsp1407I 1824 TGTACA T^GTACA
BspHI 911 TCATGA T^CATGA
BsrBI 567 CCGCTC CCGCTC(-3/-3)
BsrGI 1824 TGTACA T^GTACA
EaeI 145 TGGCCG [CT]^GGCC[GA]
Eam1105I 2425 GACCTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 2311 CCTCATCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 733 GAATTC G^AATTC
FauI 566 CCCGC CCCGC(4/6)
FbaI 2043 TGATCA T^GATCA
HincII 551 GTCAAC GT[CT]^[GA]AC
HindII 551 GTCAAC GT[CT]^[GA]AC
HindIII 2233 AAGCTT A^AGCTT
MaeII 2193 ACGT A^CGT
MslI 2409 CACAGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeI 2300 CATATG CA^TATG
PflMI 1079 CCACCATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 122 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 1015 GACTTGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 911 TCATGA T^CATGA
SfcI 309 CTGTAG C^T[GA][CT]AG
SpeI 2499 ACTAGT A^CTAGT
SspBI 1824 TGTACA T^GTACA
Van91I 1079 CCACCATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1390 GAAGTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
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AccI 467 GTATAC GT^[AC][GT]AC
2376 GTATAC
AvaII 123 GGACC G^G[AT]CC
1126 GGACC
BsaBI 361 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2038 GATTTTGATC
BsmAI 747 GTCTC GTCTC(1/5)
2195 GTCTC
BsmFI 1724 GGGAC GGGAC(10/14)
2028 GTCCC
BsmI 115 GAATGC GAATGC(1/-1)
2533 GCATTC
BspMI 2358 ACCTGC ACCTGC(4/8)
1384 GCAGGT
Bst1107I 467 GTATAC GTA^TAC
2376 GTATAC
BstEII 633 GGTCACC G^GT[ACGT]ACC
1662 GGTAACC
BstPI 633 GGTCACC G^GT[ACGT]ACC
1662 GGTAACC
BstZ17I 467 GTATAC GTA^TAC
2376 GTATAC
DraI 958 TTTAAA TTT^AAA
1926 TTTAAA
DraII 122 AGGACCC [GA]G^G[ACGT]CC[CT]
951 AGGCCCT
EcoO109I 122 AGGACCC [GA]G^G[ACGT]CC[CT]
951 AGGCCCT
EcoO65I 633 GGTCACC G^GT[ACGT]ACC
1662 GGTAACC
EcoT14I 711 CCAAGG C^C[AT][AT]GG
1964 CCTTGG
HapII 148 CCGG C^CGG
347 CCGG
HpaII 148 CCGG C^CGG
347 CCGG
MamI 361 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2038 GATTTTGATC
MfeI 141 CAATTG C^AATTG
1112 CAATTG
MspI 148 CCGG C^CGG
347 CCGG
MunI 141 CAATTG C^AATTG
1112 CAATTG
NspI 453 ACATGC [GA]CATG^[CT]
776 ACATGC
SapI 1060 GCTCTTC GCTCTTC(1/4)
2158 GCTCTTC
SmlI 390 CTCAAG C^T[CT][GA]AG
1017 CTTGAG
StyI 711 CCAAGG C^C[AT][AT]GG
1964 CCTTGG
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# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI BanII BbvI BfaI
BmyI BpmI BsaJI BsiHKAI BsiYI
BslI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII EarI EcoRII Fnu4HI FokI
HaeIII HinfI HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MspA1I MvaI MwoI
NdeII NlaIII NlaIV PleI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SspI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII