(Circular) (Six-base) MAPSORT of: pFN21AE2447.seq Check: 4763 from: 1 to: 7261 pFN21AE2447 7261 bp With 182 enzymes: SgfI * January 7, 2013 17:02 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3380 3380 Size: 7261 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5071 278 Size: 53 83 186 936 3535 2468 Fragments arranged by size: 3535 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2473 4382 4604 1937 Size: 536 1909 222 4594 Fragments arranged by size: 4594 1909 536 222 Acc65I G'GTAC_C Cuts at: 4582 4582 Size: 7261 AclI AA'CG_TT Cuts at: 1563 5388 5761 1563 Size: 3825 373 3063 Fragments arranged by size: 3825 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2082 2907 3695 4212 4418 5324 6708 6976 Size: 450 825 788 517 206 906 1384 268 Cuts at: 6976 1632 Size: 1917 Fragments arranged by size: 1917 1384 906 825 788 517 450 268 206 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7064 AflIII A'CryG_T Cuts at: 1203 6160 7137 1203 Size: 4957 977 1327 Fragments arranged by size: 4957 1327 977 AgeI A'CCGG_T Cuts at: 6092 6092 Size: 7261 AhdI GACnn_n'nnGTC Cuts at: 4435 5990 4435 Size: 1555 5706 AleI CACnn'nnGTG Cuts at: 1166 4418 1166 Size: 3252 4009 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7261 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3906 3938 3906 Size: 32 7229 AlwNI CAG_nnn'CTG Cuts at: 5004 6576 5004 Size: 1572 5689 ApaI G_GGCC'C Cuts at: 2620 2620 Size: 7261 ApaLI G'TGCA_C Cuts at: 2803 5318 6474 2803 Size: 2515 1156 3590 Fragments arranged by size: 3590 2515 1156 ApoI r'AATT_y Cuts at: 1493 2068 2335 2401 2738 3123 3609 4053 4311 Size: 575 267 66 337 385 486 444 258 Cuts at: 4311 4510 4570 4796 4948 7057 1493 Size: 199 60 226 152 2109 1697 Fragments arranged by size: 2109 1697 575 486 444 385 337 267 258 226 199 152 66 60 AseI AT'TA_AT Cuts at: 160 1032 5815 160 Size: 872 4783 1606 Fragments arranged by size: 4783 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7261 AvaI C'yCGr_G Cuts at: 1870 1943 3746 4586 1870 Size: 73 1803 840 4545 Fragments arranged by size: 4545 1803 840 73 BamHI G'GATC_C Cuts at: 2899 4591 4958 2899 Size: 1692 367 5202 Fragments arranged by size: 5202 1692 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4582 6037 618 Size: 325 217 627 2795 1455 1842 Fragments arranged by size: 2795 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2609 2620 2816 4580 1391 Size: 1218 11 196 1764 4072 Fragments arranged by size: 4072 1764 1218 196 11 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6290 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5452 5486 1903 Size: 34 50 34 3431 34 3678 Fragments arranged by size: 3678 3431 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5153 6363 5153 Size: 1210 6051 BclI T'GATC_A Cuts at: 1548 4048 1548 Size: 2500 4761 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5872 136 Size: 107 122 71 631 605 4200 1525 Fragments arranged by size: 4200 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2411 7256 2411 Size: 4845 2416 BlpI GC'TnA_GC Cuts at: 4678 4678 Size: 7261 Bme1580I G_kGCm'C Cuts at: 1790 2620 2715 2807 5322 6478 1790 Size: 830 95 92 2515 1156 2573 Fragments arranged by size: 2573 2515 1156 830 95 92 BmgBI CAC'GTC Cuts at: 1318 4199 1318 Size: 2881 4380 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3144 4992 5950 Size: 416 53 83 399 24 1727 1848 958 Cuts at: 5950 442 Size: 1753 Fragments arranged by size: 1848 1753 1727 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7261 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2888 3228 4273 4353 4408 4498 4512 5921 Size: 1649 340 1045 80 55 90 14 1409 Cuts at: 5921 1239 Size: 2579 Fragments arranged by size: 2579 1649 1409 1045 340 90 80 55 14 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2380 3043 5386 6251 6549 2380 Size: 663 2343 865 298 3092 Fragments arranged by size: 3092 2343 865 663 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5924 882 Size: 617 4425 2219 Fragments arranged by size: 4425 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5052 493 Size: 711 3848 2702 Fragments arranged by size: 3848 2702 711 BsaBI GATnn'nnATC Cuts at: 2370 4047 7255 2370 Size: 1677 3208 2376 Fragments arranged by size: 3208 2376 1677 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5068 5450 6085 275 Size: 53 83 186 936 3535 382 635 1451 Fragments arranged by size: 3535 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2351 5694 6092 6366 6513 6924 2351 Size: 3343 398 274 147 411 2688 Fragments arranged by size: 3343 2688 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2661 2691 1178 Size: 30 165 30 1258 30 5748 Fragments arranged by size: 5748 1258 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3734 1186 Size: 2548 4713 BseYI C'CCAG_C Cuts at: 6464 6960 6464 Size: 496 6765 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3036 1163 Size: 573 1300 5388 Fragments arranged by size: 5388 1300 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4711 5472 6500 1540 Size: 275 186 2710 761 1028 2301 Fragments arranged by size: 2710 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2807 3435 4396 5322 5407 6478 2807 Size: 628 961 926 85 1071 3590 Fragments arranged by size: 3590 1071 961 926 628 85 BsmI GAATG_Cn' Cuts at: 2126 4538 4772 4865 2126 Size: 2412 234 93 4522 Fragments arranged by size: 4522 2412 234 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4205 4205 Size: 7261 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2609 2620 2715 2807 2816 3435 4396 Size: 399 819 11 95 92 9 619 961 Cuts at: 4396 4580 5322 5407 6478 1391 Size: 184 742 85 1071 2174 Fragments arranged by size: 2174 1071 961 819 742 619 399 184 95 92 85 11 9 BspEI T'CCGG_A Cuts at: 6924 6924 Size: 7261 BspHI T'CATG_A Cuts at: 2916 5150 6880 2916 Size: 2234 1730 3297 Fragments arranged by size: 3297 2234 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3380 4372 4616 844 Size: 2536 992 244 3489 Fragments arranged by size: 3489 2536 992 244 BsrBI CCG'CTC Cuts at: 1394 1823 2574 5148 1394 Size: 429 751 2574 3507 Fragments arranged by size: 3507 2574 751 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5756 5930 65 Size: 1183 4508 174 1396 Fragments arranged by size: 4508 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5905 6092 1700 Size: 110 4095 187 2869 Fragments arranged by size: 4095 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3829 96 Size: 3733 3528 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7261 BssSI C'ACGA_G Cuts at: 5321 6333 5321 Size: 1012 6249 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7261 BstBI TT'CG_AA Cuts at: 6065 6065 Size: 7261 BstEII G'GTnAC_C Cuts at: 1184 2638 3667 1184 Size: 1454 1029 4778 Fragments arranged by size: 4778 1454 1029 BstYI r'GATC_y Cuts at: 1972 2299 2411 2490 2899 4591 4958 5343 5360 Size: 327 112 79 409 1692 367 385 17 Cuts at: 5360 6812 6898 6910 7256 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1692 1452 409 385 367 346 327 112 86 79 17 12 BstZ17I GTA'TAC Cuts at: 2474 4383 2474 Size: 1909 5352 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6709 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6522 Fragments arranged by size: 6522 683 56 BtsI GCAGTG_nn' Cuts at: 3687 3832 4777 5571 5591 3687 Size: 145 945 794 20 5357 Fragments arranged by size: 5357 945 794 145 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7261 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3100 3135 529 Size: 35 2536 35 4655 Fragments arranged by size: 4655 2536 35 35 DraI TTT'AAA Cuts at: 2965 3933 4565 2965 Size: 968 632 5661 Fragments arranged by size: 5661 968 632 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6268 809 Size: 507 4952 1802 Fragments arranged by size: 4952 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2150 4708 5597 Size: 54 1425 50 275 18 320 2558 889 Cuts at: 5597 7226 8 Size: 1629 43 Fragments arranged by size: 2558 1629 1425 889 320 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4708 1537 Size: 275 2896 4090 Fragments arranged by size: 4090 2896 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2264 3072 4170 5191 1353 Size: 911 808 1098 1021 3423 Fragments arranged by size: 3423 1098 1021 911 808 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2763 5848 6222 6368 387 Size: 1064 138 1174 3085 374 146 1280 Fragments arranged by size: 3085 1280 1174 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2082 2888 2907 3228 3695 4212 4273 Size: 393 450 806 19 321 467 517 61 Cuts at: 4273 4353 4408 4418 4498 4512 5324 5921 6708 Size: 80 55 10 80 14 812 597 787 Cuts at: 6708 6976 1239 Size: 268 1524 Fragments arranged by size: 1524 812 806 787 597 517 467 450 393 321 268 80 80 61 55 19 14 10 EcoNI CCTnn'n_nnAGG Cuts at: 4320 4320 Size: 7261 EcoO109I rG'GnC_Cy Cuts at: 2128 2957 2128 Size: 829 6432 EcoRI G'AATT_C Cuts at: 2738 4570 4948 2738 Size: 1832 378 5051 Fragments arranged by size: 5051 1832 378 FspI TGC'GCA Cuts at: 5767 5767 Size: 7261 HaeII r_GCGC'y Cuts at: 1678 6408 1678 Size: 4730 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4055 4087 4421 4453 5902 Size: 32 135 32 2483 32 334 32 1449 Cuts at: 5902 5934 5976 6008 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2483 1449 334 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2558 4605 4851 669 Size: 988 281 620 2047 246 3079 Fragments arranged by size: 3079 2047 988 620 281 246 HindIII A'AGCT_T Cuts at: 748 4238 748 Size: 3490 3771 HpaI GTT'AAC Cuts at: 4851 4851 Size: 7261 KpnI G_GTAC'C Cuts at: 4586 4586 Size: 7261 MfeI C'AATT_G Cuts at: 2146 3117 4860 2146 Size: 971 1743 4547 Fragments arranged by size: 4547 1743 971 MluI A'CGCG_T Cuts at: 7137 7137 Size: 7261 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2802 3363 3548 6375 6559 1305 Size: 1497 561 185 2827 184 2007 Fragments arranged by size: 2827 2007 1497 561 185 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5439 Fragments arranged by size: 5439 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4418 5219 5578 5737 518 Size: 648 3252 801 359 159 2042 Fragments arranged by size: 3252 2042 801 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2566 3080 4232 4677 5354 6502 6747 1606 Size: 960 514 1152 445 677 1148 245 2120 Fragments arranged by size: 2120 1152 1148 960 677 514 445 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6709 NdeI CA'TA_TG Cuts at: 387 4306 387 Size: 3919 3342 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7261 NotI GC'GGCC_GC Cuts at: 4708 4708 Size: 7261 NspI r_CATG'y Cuts at: 1132 1229 2462 2785 4619 6164 1132 Size: 97 1233 323 1834 1545 2229 Fragments arranged by size: 2229 1834 1545 1233 323 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7261 PciI A'CATG_T Cuts at: 6160 6160 Size: 7261 PflMI CCAn_nnn'nTGG Cuts at: 1108 3090 1108 Size: 1982 5279 PmeI GTTT'AAAC Cuts at: 4565 4565 Size: 7261 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5330 5362 6843 6875 5330 Size: 32 1481 32 5716 Fragments arranged by size: 5716 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2128 2128 Size: 7261 PshAI GACnn'nnGTC Cuts at: 1613 3024 1613 Size: 1411 5850 PsiI TTA'TAA Cuts at: 2538 2991 4094 4831 2538 Size: 453 1103 737 4968 Fragments arranged by size: 4968 1103 737 453 PspOMI G'GGCC_C Cuts at: 2616 2616 Size: 7261 PstI C_TGCA'G Cuts at: 830 4613 830 Size: 3783 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7261 PvuII CAG'CTG Cuts at: 2566 3080 4232 2566 Size: 514 1152 5595 Fragments arranged by size: 5595 1152 514 SalI G'TCGA_C Cuts at: 1936 4603 1936 Size: 2667 4594 SapI GCTCTTCn'nnn_ Cuts at: 3072 4170 3072 Size: 1098 6163 SbfI CC_TGCA'GG Cuts at: 4613 4613 Size: 7261 ScaI AGT'ACT Cuts at: 5509 7221 5509 Size: 1712 5549 SfcI C'TryA_G Cuts at: 826 1045 2314 4609 5744 6425 6616 826 Size: 219 1269 2295 1135 681 191 1471 Fragments arranged by size: 2295 1471 1269 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7261 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7261 SmaI CCC'GGG Cuts at: 1872 4588 1872 Size: 2716 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2395 3022 5365 6266 6528 820 Size: 197 926 452 627 2343 901 262 1553 Fragments arranged by size: 2343 1553 926 901 627 452 262 197 SnaBI TAC'GTA Cuts at: 493 5052 493 Size: 4559 2702 SpeI A'CTAG_T Cuts at: 152 4504 152 Size: 4352 2909 SphI G_CATG'C Cuts at: 1132 4619 1132 Size: 3487 3774 SspI AAT'ATT Cuts at: 5 52 2113 2281 3719 5185 5 Size: 47 2061 168 1438 1466 2081 Fragments arranged by size: 2081 2061 1466 1438 168 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7261 StyI C'CwwG_G Cuts at: 513 1065 2716 3969 4700 513 Size: 552 1651 1253 731 3074 Fragments arranged by size: 3074 1651 1253 731 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2022 5288 5305 5458 5643 6088 325 Size: 1255 442 3266 17 153 185 445 1498 Fragments arranged by size: 3266 1498 1255 445 442 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2677 3829 5507 Size: 274 80 33 51 1443 700 1152 1678 Cuts at: 5507 7219 96 Size: 1712 138 Fragments arranged by size: 1712 1678 1443 1152 700 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4188 4220 7221 7253 4188 Size: 32 3001 32 4196 Fragments arranged by size: 4196 3001 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7261 XbaI T'CTAG_A Cuts at: 4597 4597 Size: 7261 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7261 XmaI C'CCGG_G Cuts at: 1870 4586 1870 Size: 2716 4545 XmnI GAAnn'nnTTC Cuts at: 3399 5390 3399 Size: 1991 5270 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5069 276 Size: 53 83 186 936 3535 2468 Fragments arranged by size: 3535 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI