Restriction map for
pFN21AE1701
(Linear) (Six-base) MAPSORT of: KIEE1701.seq Check: 5499 from: 1 to: 618
KIEE1701.orf
With 182 enzymes: SgfI *
July 15, 2010 18:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 558 618
Size: 558 60
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 618
Size: 304 314
AflIII A'CryG_T
Cuts at: 0 102 618
Size: 102 516
ApaLI G'TGCA_C
Cuts at: 0 264 618
Size: 264 354
AvaI C'yCGr_G
Cuts at: 0 206 618
Size: 206 412
BanII G_rGCy'C
Cuts at: 0 382 618
Size: 382 236
BfrBI ATG'CAT
Cuts at: 0 44 618
Size: 44 574
BglI GCCn_nnn'nGGC
Cuts at: 0 446 618
Size: 446 172
Bme1580I G_kGCm'C
Cuts at: 0 268 618
Size: 268 350
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 387 618
Size: 387 231
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 280 310 379 409 618
Size: 280 30 69 30 209
Fragments arranged by size:
280 209 69 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 359 390 618
Size: 359 31 228
Fragments arranged by size:
359 228 31
BseYI C'CCAG_C
Cuts at: 0 130 618
Size: 130 488
BsiHKAI G_wGCw'C
Cuts at: 0 268 329 382 618
Size: 268 61 53 236
Fragments arranged by size:
268 236 61 53
Bsp1286I G_dGCh'C
Cuts at: 0 268 329 382 618
Size: 268 61 53 236
Fragments arranged by size:
268 236 61 53
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 558 618
Size: 558 60
BsrBI CCG'CTC
Cuts at: 0 579 618
Size: 579 39
BtsI GCAGTG_nn'
Cuts at: 0 442 479 618
Size: 442 37 139
Fragments arranged by size:
442 139 37
DraI TTT'AAA
Cuts at: 0 72 618
Size: 72 546
EaeI y'GGCC_r
Cuts at: 0 258 618
Size: 258 360
EcoICRI GAG'CTC
Cuts at: 0 380 618
Size: 380 238
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 387 618
Size: 304 83 231
Fragments arranged by size:
304 231 83
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 529 561 618
Size: 529 32 57
Fragments arranged by size:
529 57 32
MscI TGG'CCA
Cuts at: 0 260 618
Size: 260 358
MslI CAynn'nnrTG
Cuts at: 0 49 618
Size: 49 569
MspA1I CmG'CkG
Cuts at: 0 432 618
Size: 432 186
NsiI A_TGCA'T
Cuts at: 0 46 618
Size: 46 572
NspI r_CATG'y
Cuts at: 0 44 106 546 618
Size: 44 62 440 72
Fragments arranged by size:
440 72 62 44
PciI A'CATG_T
Cuts at: 0 102 618
Size: 102 516
PstI C_TGCA'G
Cuts at: 0 437 618
Size: 437 181
PvuII CAG'CTG
Cuts at: 0 432 618
Size: 432 186
SacI G_AGCT'C
Cuts at: 0 382 618
Size: 382 236
ScaI AGT'ACT
Cuts at: 0 364 618
Size: 364 254
SfcI C'TryA_G
Cuts at: 0 433 618
Size: 433 185
SphI G_CATG'C
Cuts at: 0 44 618
Size: 44 574
TatI w'GTAC_w
Cuts at: 0 362 618
Size: 362 256
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 280 312 379 411 505 537 618
Size: 280 32 67 32 94 32 81
Fragments arranged by size:
280 94 81 67 32 32 32
Enzymes that do cut:
AarI AcuI AflIII ApaLI AvaI BanII BfrBI BglI
Bme1580I BpmI BsaXI BseRI BseYI BsiHKAI Bsp1286I BspMI
BsrBI BtsI DraI EaeI EcoICRI Eco57MI Hin4I MscI
MslI MspA1I NsiI NspI PciI PstI PvuII SacI
ScaI SfcI SphI TatI TstI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BbsI BbvCI
BcgI BciVI BclI BglII BlpI BmgBI BmrI BmtI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraIII
DrdI EagI EarI EciI EcoNI EcoO109I EcoRI EcoRV
FalI FseI FspI FspAI HaeII HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI PacI PasI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SanDI SapI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SrfI SspI StuI StyI SwaI TaqII
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI