(Circular) (Six-base) MAPSORT of: pFN21AE1805.seq Check: 7891 from: 1 to: 5665 pFN21AE1805 5665 bp With 182 enzymes: SgfI * August 16, 2010 14:53 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2078 2078 Size: 5665 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3475 278 Size: 53 83 186 936 1939 2468 Fragments arranged by size: 2468 1939 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3008 1937 Size: 1071 4594 Acc65I G'GTAC_C Cuts at: 2986 2986 Size: 5665 AclI AA'CG_TT Cuts at: 1563 3792 4165 1563 Size: 2229 373 3063 Fragments arranged by size: 3063 2229 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2425 3728 5112 5380 1632 Size: 793 1303 1384 268 1917 Fragments arranged by size: 1917 1384 1303 793 268 AflII C'TTAA_G Cuts at: 820 1017 2960 820 Size: 197 1943 3525 Fragments arranged by size: 3525 1943 197 AflIII A'CryG_T Cuts at: 1203 4564 5541 1203 Size: 3361 977 1327 Fragments arranged by size: 3361 1327 977 AgeI A'CCGG_T Cuts at: 4496 4496 Size: 5665 AhdI GACnn_n'nnGTC Cuts at: 4394 4394 Size: 5665 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5665 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5665 AlwNI CAG_nnn'CTG Cuts at: 3408 4980 3408 Size: 1572 4093 ApaLI G'TGCA_C Cuts at: 2093 2459 3722 4878 2093 Size: 366 1263 1156 2880 Fragments arranged by size: 2880 1263 1156 366 ApoI r'AATT_y Cuts at: 1493 2329 2597 2974 3200 3352 5461 1493 Size: 836 268 377 226 152 2109 1697 Fragments arranged by size: 2109 1697 836 377 268 226 152 AscI GG'CGCG_CC Cuts at: 2043 2043 Size: 5665 AseI AT'TA_AT Cuts at: 160 1032 4219 160 Size: 872 3187 1606 Fragments arranged by size: 3187 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5665 AvaI C'yCGr_G Cuts at: 1870 1943 2990 1870 Size: 73 1047 4545 Fragments arranged by size: 4545 1047 73 BamHI G'GATC_C Cuts at: 2995 3362 2995 Size: 367 5298 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2986 4441 618 Size: 325 217 627 1199 1455 1842 Fragments arranged by size: 1842 1455 1199 627 325 217 BanII G_rGCy'C Cuts at: 1391 2298 2804 2984 1391 Size: 907 506 180 4072 Fragments arranged by size: 4072 907 506 180 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2591 928 Size: 971 692 4002 Fragments arranged by size: 4002 971 692 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3856 3890 1903 Size: 34 50 34 1835 34 3678 Fragments arranged by size: 3678 1835 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3557 4767 3557 Size: 1210 4455 BclI T'GATC_A Cuts at: 1548 2373 1548 Size: 825 4840 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4276 136 Size: 107 122 71 631 605 2604 1525 Fragments arranged by size: 2604 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5660 5660 Size: 5665 BlpI GC'TnA_GC Cuts at: 3082 3082 Size: 5665 Bme1580I G_kGCm'C Cuts at: 1790 2097 2463 2621 3726 4882 1790 Size: 307 366 158 1105 1156 2573 Fragments arranged by size: 2573 1156 1105 366 307 158 BmgBI CAC'GTC Cuts at: 1318 2839 1318 Size: 1521 4144 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3396 4354 442 Size: 416 53 83 399 24 1979 958 1753 Fragments arranged by size: 1979 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5665 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2843 4325 1239 Size: 1604 1482 2579 Fragments arranged by size: 2579 1604 1482 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2189 2486 2936 3790 4655 4953 2189 Size: 297 450 854 865 298 2901 Fragments arranged by size: 2901 865 854 450 298 297 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4328 882 Size: 617 2829 2219 Fragments arranged by size: 2829 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2134 3456 493 Size: 711 930 1322 2702 Fragments arranged by size: 2702 1322 930 711 BsaBI GATnn'nnATC Cuts at: 5659 5659 Size: 5665 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3472 3854 4489 275 Size: 53 83 186 936 1939 382 635 1451 Fragments arranged by size: 1939 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4098 4496 4770 4917 5328 4098 Size: 398 274 147 411 4435 Fragments arranged by size: 4435 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2375 2405 2453 2483 1178 Size: 30 165 30 972 30 48 30 4360 Fragments arranged by size: 4360 972 165 48 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2461 1186 Size: 1275 4390 BseYI C'CCAG_C Cuts at: 2113 2551 2709 2813 4868 5364 2113 Size: 438 158 104 2055 496 2414 Fragments arranged by size: 2414 2055 496 438 158 104 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2156 2402 2921 1163 Size: 573 420 246 519 3907 Fragments arranged by size: 3907 573 519 420 246 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3115 3876 4904 1540 Size: 275 186 1114 761 1028 2301 Fragments arranged by size: 2301 1114 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2097 2439 2463 3726 3811 4882 2097 Size: 342 24 1263 85 1071 2880 Fragments arranged by size: 2880 1263 1071 342 85 24 BsmI GAATG_Cn' Cuts at: 3176 3269 3176 Size: 93 5572 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2097 2298 2439 2463 2621 2804 2984 Size: 399 307 201 141 24 158 183 180 Cuts at: 2984 3726 3811 4882 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 307 201 183 180 158 141 85 24 BspEI T'CCGG_A Cuts at: 5328 5328 Size: 5665 BspHI T'CATG_A Cuts at: 3554 5284 3554 Size: 1730 3935 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2078 3020 844 Size: 1234 942 3489 Fragments arranged by size: 3489 1234 942 BsrBI CCG'CTC Cuts at: 1394 1823 2775 3552 1394 Size: 429 952 777 3507 Fragments arranged by size: 3507 952 777 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2640 4160 4334 65 Size: 1183 1392 1520 174 1396 Fragments arranged by size: 1520 1396 1392 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2079 4309 4496 1700 Size: 110 269 2230 187 2869 Fragments arranged by size: 2869 2230 269 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5665 BssHII G'CGCG_C Cuts at: 1925 2043 1925 Size: 118 5547 BssSI C'ACGA_G Cuts at: 3725 4737 3725 Size: 1012 4653 BstAPI GCAn_nnn'nTGC Cuts at: 794 2425 794 Size: 1631 4034 BstBI TT'CG_AA Cuts at: 4469 4469 Size: 5665 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5665 BstXI CCAn_nnnn'nTGG Cuts at: 2320 2320 Size: 5665 BstYI r'GATC_y Cuts at: 1972 2995 3362 3747 3764 5216 5302 5314 5660 Size: 1023 367 385 17 1452 86 12 346 Cuts at: 5660 1972 Size: 1977 Fragments arranged by size: 1977 1452 1023 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2454 513 Size: 552 1389 3724 Fragments arranged by size: 3724 1389 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4926 Fragments arranged by size: 4926 683 56 BtsI GCAGTG_nn' Cuts at: 2884 3181 3975 3995 2884 Size: 297 794 20 4554 Fragments arranged by size: 4554 794 297 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5665 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5630 DraI TTT'AAA Cuts at: 2193 2969 2193 Size: 776 4889 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4672 809 Size: 507 3356 1802 Fragments arranged by size: 3356 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2128 2668 3112 Size: 54 1425 50 275 18 298 540 444 Cuts at: 3112 4001 5630 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 540 444 298 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3112 1537 Size: 275 1300 4090 Fragments arranged by size: 4090 1300 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2136 2792 3595 1353 Size: 783 656 803 3423 Fragments arranged by size: 3423 803 783 656 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4252 4626 4772 387 Size: 1064 138 2663 374 146 1280 Fragments arranged by size: 2663 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2425 2843 3728 4325 5112 5380 1239 Size: 393 793 418 885 597 787 268 1524 Fragments arranged by size: 1524 885 793 787 597 418 393 268 EcoO109I rG'GnC_Cy Cuts at: 2446 2446 Size: 5665 EcoRI G'AATT_C Cuts at: 2329 2974 3352 2329 Size: 645 378 4642 Fragments arranged by size: 4642 645 378 FspI TGC'GCA Cuts at: 4171 4171 Size: 5665 HaeII r_GCGC'y Cuts at: 1678 2120 2127 2360 4812 1678 Size: 442 7 233 2452 2531 Fragments arranged by size: 2531 2452 442 233 7 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2216 2248 4306 4338 4380 Size: 32 135 32 644 32 2058 32 42 Cuts at: 4380 4412 1373 Size: 32 2626 Fragments arranged by size: 2626 2058 644 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2689 3009 3255 669 Size: 988 281 751 320 246 3079 Fragments arranged by size: 3079 988 751 320 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5665 HpaI GTT'AAC Cuts at: 3255 3255 Size: 5665 KpnI G_GTAC'C Cuts at: 2990 2990 Size: 5665 MfeI C'AATT_G Cuts at: 3264 3264 Size: 5665 MluI A'CGCG_T Cuts at: 5541 5541 Size: 5665 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4779 4963 1305 Size: 3474 184 2007 Fragments arranged by size: 3474 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2130 2670 10 Size: 54 1425 343 298 540 3005 Fragments arranged by size: 3005 1425 540 343 298 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2072 3623 3982 4141 518 Size: 648 843 63 1551 359 159 2042 Fragments arranged by size: 2042 1551 843 648 359 159 63 MspA1I CmG'CkG Cuts at: 1606 2551 2765 3081 3758 4906 5151 1606 Size: 945 214 316 677 1148 245 2120 Fragments arranged by size: 2120 1148 945 677 316 245 214 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5113 NdeI CA'TA_TG Cuts at: 387 387 Size: 5665 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5665 NotI GC'GGCC_GC Cuts at: 3112 3112 Size: 5665 NspI r_CATG'y Cuts at: 1132 1229 2077 3023 4568 1132 Size: 97 848 946 1545 2229 Fragments arranged by size: 2229 1545 946 848 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5665 PciI A'CATG_T Cuts at: 4564 4564 Size: 5665 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5665 PmeI GTTT'AAAC Cuts at: 2969 2969 Size: 5665 PmlI CAC'GTG Cuts at: 2134 2134 Size: 5665 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3734 3766 5247 5279 3734 Size: 32 1481 32 4120 Fragments arranged by size: 4120 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2446 2446 Size: 5665 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5665 PsiI TTA'TAA Cuts at: 3235 3235 Size: 5665 PstI C_TGCA'G Cuts at: 830 2221 2879 3017 830 Size: 1391 658 138 3478 Fragments arranged by size: 3478 1391 658 138 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5665 PvuII CAG'CTG Cuts at: 2551 2551 Size: 5665 SalI G'TCGA_C Cuts at: 1936 3007 1936 Size: 1071 4594 SapI GCTCTTCn'nnn_ Cuts at: 2792 2792 Size: 5665 SbfI CC_TGCA'GG Cuts at: 3017 3017 Size: 5665 ScaI AGT'ACT Cuts at: 3913 5625 3913 Size: 1712 3953 SexAI A'CCwGG_T Cuts at: 2016 2016 Size: 5665 SfcI C'TryA_G Cuts at: 826 1045 2217 2875 3013 4148 4829 5020 826 Size: 219 1172 658 138 1135 681 191 1471 Fragments arranged by size: 1471 1172 1135 681 658 219 191 138 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5665 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5665 SmaI CCC'GGG Cuts at: 1872 2992 1872 Size: 1120 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2168 2501 2951 2960 3769 4670 Size: 197 926 225 333 450 9 809 901 Cuts at: 4670 4932 820 Size: 262 1553 Fragments arranged by size: 1553 926 901 809 450 333 262 225 197 9 SnaBI TAC'GTA Cuts at: 493 3456 493 Size: 2963 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5665 SphI G_CATG'C Cuts at: 1132 3023 1132 Size: 1891 3774 SspI AAT'ATT Cuts at: 5 52 3589 5 Size: 47 3537 2081 Fragments arranged by size: 3537 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5665 StyI C'CwwG_G Cuts at: 513 1065 2804 3104 513 Size: 552 1739 300 3074 Fragments arranged by size: 3074 1739 552 300 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2464 3692 3709 3862 4047 4492 325 Size: 1255 884 1228 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1228 884 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2854 3911 5623 Size: 274 80 33 51 1443 877 1057 1712 Cuts at: 5623 96 Size: 138 Fragments arranged by size: 1712 1443 1057 877 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2453 2485 5625 5657 2453 Size: 32 3140 32 2461 Fragments arranged by size: 3140 2461 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5665 XbaI T'CTAG_A Cuts at: 3001 3001 Size: 5665 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2317 2317 Size: 5665 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5665 XmaI C'CCGG_G Cuts at: 1870 2990 1870 Size: 1120 4545 XmnI GAAnn'nnTTC Cuts at: 2496 3794 2496 Size: 1298 4367 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3473 276 Size: 53 83 186 936 1939 2468 Fragments arranged by size: 2468 1939 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI