################################## # NO CUT ENZYMES ( 116 enzymes) # ################################## Acc65I AccIII AclI AcsI AflII AgeI AhdI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsaI BsaWI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BsrBI BsrDI BsrGI Bst1107I BstAPI BstBI BstZ17I CelII ClaI CpoI DraI Eam1105I Eco47III EcoNI EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KasI KpnI MaeI MamI MfeI MluI MluNI MroI MscI MseI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI SwaI TfiI Tru9I Tsp509I XbaI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AatII 871 GACGTC GACGT^C AccI 1303 GTCTAC GT^[AC][GT]AC Alw44I 847 GTGCAC G^TGCAC AlwNI 450 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 132 CCTTAGG CC^T[ACGT]AGG ApaI 432 GGGCCC GGGCC^C ApaLI 847 GTGCAC G^TGCAC BbsI 1276 GTCTTC GAAGAC(2/6) BciVI 162 GGATAC GTATCC(6/5) BglI 803 GCCACCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 1276 GTCTTC GAAGAC(2/6) BsaAI 1039 TACGTG [CT]AC^GT[GA] BsiEI 790 CGGCCG CG[GA][CT]^CG BsmAI 384 GAGAC GTCTC(1/5) BspLU11I 1472 ACATGT A^CATGT BsrFI 946 ACCGGC [GA]^CCGG[CT] BssHII 681 GCGCGC G^CGCGC BssSI 937 CACGAG CACGAG(-5/-1) BstEII 942 GGTGACC G^GT[ACGT]ACC BstPI 942 GGTGACC G^GT[ACGT]ACC Bsu36I 132 CCTTAGG CC^T[ACGT]AGG Cfr10I 946 ACCGGC [GA]^CCGG[CT] DrdI 1485 GACGCTGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 790 CGGCCG C^GGCCG EclXI 790 CGGCCG C^GGCCG Eco52I 790 CGGCCG C^GGCCG Eco81I 132 CCTTAGG CC^T[ACGT]AGG EcoO65I 942 GGTGACC G^GT[ACGT]ACC KspI 982 CCGCGG CCGC^GG PaeR7I 1120 CTCGAG C^TCGAG PflMI 1245 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 301 GGGACCT [GA]G^G[AT]CC[CT] PstI 469 CTGCAG CTGCA^G SacII 982 CCGCGG CCGC^GG SmaI 57 CCCGGG CCC^GGG SphI 1316 GCATGC GCATG^C Van91I 1245 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1163 CCATCCTCATCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 1120 CTCGAG C^TCGAG XmaCI 57 CCCGGG C^CCGGG XmaI 57 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AcyI 145 GGCGTC G[GA]^CG[CT]C 871 GACGTC AflIII 461 ACGTGT A^C[GA][CT]GT 1472 ACATGT AspI 856 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1461 GACCGTGTC BanI 520 GGCACC G^G[CT][GA]CC 1240 GGCACC BcgI 659 CGAAGCTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 761 GCAGCCGCTTCG BpmI 97 CTGGAG CTGGAG(16/14) 721 CTGGAG BsaHI 145 GGCGTC G[GA]^CG[CT]C 871 GACGTC BseRI 28 GAGGAG GAGGAG(10/8) 100 GAGGAG BspMI 281 ACCTGC ACCTGC(4/8) 1247 ACCTGC BstXI 500 CCATCAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1400 CCATGATCCTGG BstYI 1130 AGATCC [GA]^GATC[CT] 1208 AGATCC DraIII 937 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG 1098 CACGCTGTG EcoT14I 428 CCTTGG C^C[AT][AT]GG 988 CCAAGG HaeII 20 AGCGCC [GA]GCGC^[CT] 717 GGCGCT Hin1I 145 GGCGTC G[GA]^CG[CT]C 871 GACGTC HinfI 913 GAGTC G^A[ACGT]TC 1123 GAGTC MflI 1130 AGATCC [GA]^GATC[CT] 1208 AGATCC PflFI 856 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1461 GACCGTGTC PleI 913 GAGTC GAGTC(4/5) 1123 GAGTC SapI 474 GCTCTTC GCTCTTC(1/4) 774 GCTCTTC SfcI 469 CTGCAG C^T[GA][CT]AG 1515 CTACAG SmlI 25 CTTGAG C^T[CT][GA]AG 1120 CTCGAG StyI 428 CCTTGG C^C[AT][AT]GG 988 CCAAGG Tth111I 856 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1461 GACCGTGTC XhoII 1130 AGATCC [GA]^GATC[CT] 1208 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 76 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AspHI AvaI AvaII BanII BbvI BcnI BmyI BsaJI BsgI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI StuI TaqI TseI Tsp45I TspRI TthHB8I