(Circular) (Six-base) MAPSORT of: pFN21AE2614.seq Check: 4457 from: 1 to: 6295 pFN21AE2614 6295 bp With 182 enzymes: SgfI * March 25, 2016 16:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3261 3261 Size: 6295 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2880 4105 278 Size: 53 83 186 936 1344 1225 2468 Fragments arranged by size: 2468 1344 1225 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3309 3638 1937 Size: 1372 329 4594 Fragments arranged by size: 4594 1372 329 Acc65I G'GTAC_C Cuts at: 3616 3616 Size: 6295 AclI AA'CG_TT Cuts at: 1563 4422 4795 1563 Size: 2859 373 3063 Fragments arranged by size: 3063 2859 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3004 4358 5742 6010 1632 Size: 1372 1354 1384 268 1917 Fragments arranged by size: 1917 1384 1372 1354 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6098 AflIII A'CryG_T Cuts at: 1203 2466 3477 5194 6171 1203 Size: 1263 1011 1717 977 1327 Fragments arranged by size: 1717 1327 1263 1011 977 AgeI A'CCGG_T Cuts at: 5126 5126 Size: 6295 AhdI GACnn_n'nnGTC Cuts at: 5024 5024 Size: 6295 AleI CACnn'nnGTG Cuts at: 1166 2276 1166 Size: 1110 5185 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3269 3569 1184 Size: 2085 300 3910 Fragments arranged by size: 3910 2085 300 AlwNI CAG_nnn'CTG Cuts at: 2460 4038 5610 2460 Size: 1578 1572 3145 Fragments arranged by size: 3145 1578 1572 ApaI G_GGCC'C Cuts at: 2441 2441 Size: 6295 ApaLI G'TGCA_C Cuts at: 2852 4352 5508 2852 Size: 1500 1156 3639 Fragments arranged by size: 3639 1500 1156 ApoI r'AATT_y Cuts at: 1493 3604 3830 3982 6091 1493 Size: 2111 226 152 2109 1697 Fragments arranged by size: 2111 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4849 160 Size: 872 3817 1606 Fragments arranged by size: 3817 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6295 AvaI C'yCGr_G Cuts at: 1870 1943 2062 2487 3125 3620 1870 Size: 73 119 425 638 495 4545 Fragments arranged by size: 4545 638 495 425 119 73 BamHI G'GATC_C Cuts at: 3625 3992 3625 Size: 367 5928 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2525 3245 3616 5071 618 Size: 325 217 627 738 720 371 1455 1842 Fragments arranged by size: 1842 1455 738 720 627 371 325 217 BanII G_rGCy'C Cuts at: 1391 2012 2040 2441 3028 3434 3614 1391 Size: 621 28 401 587 406 180 4072 Fragments arranged by size: 4072 621 587 406 401 180 28 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3274 928 Size: 971 1375 3949 Fragments arranged by size: 3949 1375 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2653 2687 2755 2789 4486 4520 1903 Size: 34 716 34 68 34 1697 34 3678 Fragments arranged by size: 3678 1697 716 68 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2161 4187 5397 2161 Size: 2026 1210 3059 Fragments arranged by size: 3059 2026 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6295 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2814 4906 136 Size: 107 122 71 631 605 1142 2092 1525 Fragments arranged by size: 2092 1525 1142 631 605 122 107 71 BglII A'GATC_T Cuts at: 6290 6290 Size: 6295 BlpI GC'TnA_GC Cuts at: 3712 3712 Size: 6295 Bme1580I G_kGCm'C Cuts at: 1790 2193 2441 2528 2856 3248 3405 4356 5512 Size: 403 248 87 328 392 157 951 1156 Cuts at: 5512 1790 Size: 2573 Fragments arranged by size: 2573 1156 951 403 392 328 248 157 87 BmgBI CAC'GTC Cuts at: 1318 2278 2467 1318 Size: 960 189 5146 Fragments arranged by size: 5146 960 189 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4026 4984 442 Size: 416 53 83 399 24 2609 958 1753 Fragments arranged by size: 2609 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6295 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2123 2747 4955 1239 Size: 884 624 2208 2579 Fragments arranged by size: 2579 2208 884 624 Bpu10I CC'TnA_GC Cuts at: 2124 2124 Size: 6295 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2051 4420 5285 5583 2051 Size: 2369 865 298 2763 Fragments arranged by size: 2763 2369 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4958 882 Size: 617 3459 2219 Fragments arranged by size: 3459 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3046 4086 493 Size: 711 1842 1040 2702 Fragments arranged by size: 2702 1842 1040 711 BsaBI GATnn'nnATC Cuts at: 6289 6289 Size: 6295 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2151 2877 4102 4484 Size: 53 83 186 936 618 726 1225 382 Cuts at: 4484 5119 275 Size: 635 1451 Fragments arranged by size: 1451 1225 936 726 635 618 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4728 5126 5400 5547 5958 4728 Size: 398 274 147 411 5065 Fragments arranged by size: 5065 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2720 2750 3062 3092 1178 Size: 30 165 30 1317 30 312 30 4381 Fragments arranged by size: 4381 1317 312 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2048 2120 1186 Size: 862 72 5361 Fragments arranged by size: 5361 862 72 BseYI C'CCAG_C Cuts at: 2039 2155 2893 2935 3314 3493 5498 5994 2039 Size: 116 738 42 379 179 2005 496 2340 Fragments arranged by size: 2340 2005 738 496 379 179 116 42 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2384 2527 2570 2732 3020 3499 3539 Size: 573 648 143 43 162 288 479 40 Cuts at: 3539 1163 Size: 3919 Fragments arranged by size: 3919 648 573 479 288 162 143 43 40 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2798 3745 4506 5534 1540 Size: 275 186 797 947 761 1028 2301 Fragments arranged by size: 2301 1028 947 797 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2627 2781 2856 2892 4356 4441 5512 2627 Size: 154 75 36 1464 85 1071 3410 Fragments arranged by size: 3410 1464 1071 154 85 75 36 BsmI GAATG_Cn' Cuts at: 3806 3899 3806 Size: 93 6202 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2040 2193 2441 2528 2627 2781 Size: 399 222 28 153 248 87 99 154 Cuts at: 2781 2856 2892 3028 3248 3405 3434 3614 4356 Size: 75 36 136 220 157 29 180 742 Cuts at: 4356 4441 5512 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 248 222 220 180 157 154 153 136 99 87 85 75 36 29 28 BspEI T'CCGG_A Cuts at: 5958 5958 Size: 6295 BspHI T'CATG_A Cuts at: 4184 5914 4184 Size: 1730 4565 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2295 3261 3650 844 Size: 1451 966 389 3489 Fragments arranged by size: 3489 1451 966 389 BsrBI CCG'CTC Cuts at: 1394 1823 4182 1394 Size: 429 2359 3507 Fragments arranged by size: 3507 2359 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4790 4964 65 Size: 1183 3542 174 1396 Fragments arranged by size: 3542 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2951 4939 5126 1700 Size: 110 1141 1988 187 2869 Fragments arranged by size: 2869 1988 1141 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6295 BssHII G'CGCG_C Cuts at: 1925 2686 1925 Size: 761 5534 BssSI C'ACGA_G Cuts at: 2942 4355 5367 2942 Size: 1413 1012 3870 Fragments arranged by size: 3870 1413 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6295 BstBI TT'CG_AA Cuts at: 5099 5099 Size: 6295 BstEII G'GTnAC_C Cuts at: 1184 2947 1184 Size: 1763 4532 BstXI CCAn_nnnn'nTGG Cuts at: 2512 3412 2512 Size: 900 5395 BstYI r'GATC_y Cuts at: 1972 3135 3213 3625 3992 4377 4394 5846 5932 Size: 1163 78 412 367 385 17 1452 86 Cuts at: 5932 5944 6290 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1163 412 385 367 346 86 78 17 12 Bsu36I CC'TnA_GG Cuts at: 2138 2138 Size: 6295 BtgI C'CryG_G Cuts at: 513 1065 2004 2013 2529 2987 3231 3504 513 Size: 552 939 9 516 458 244 273 3304 Fragments arranged by size: 3304 939 552 516 458 273 244 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2237 3067 487 Size: 683 56 1011 830 3715 Fragments arranged by size: 3715 1011 830 683 56 BtsI GCAGTG_nn' Cuts at: 3142 3362 3811 4605 4625 3142 Size: 220 449 794 20 4812 Fragments arranged by size: 4812 794 449 220 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6295 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6260 DraI TTT'AAA Cuts at: 3599 3599 Size: 6295 DraIII CAC_nnn'GTG Cuts at: 2947 3108 2947 Size: 161 6134 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3496 5302 809 Size: 507 2180 1806 1802 Fragments arranged by size: 2180 1806 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2514 2795 2990 Size: 54 1425 50 275 18 684 281 195 Cuts at: 2990 3742 4631 6260 8 Size: 752 889 1629 43 Fragments arranged by size: 1629 1425 889 752 684 281 275 195 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2795 3742 1537 Size: 275 983 947 4090 Fragments arranged by size: 4090 983 947 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2486 2786 3299 4225 1353 Size: 1133 300 513 926 3423 Fragments arranged by size: 3423 1133 926 513 300 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2129 3128 3490 4882 5256 5402 Size: 1064 138 540 999 362 1392 374 146 Cuts at: 5402 387 Size: 1280 Fragments arranged by size: 1392 1280 1064 999 540 374 362 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2123 2747 3004 4358 4955 5742 6010 Size: 393 491 624 257 1354 597 787 268 Cuts at: 6010 1239 Size: 1524 Fragments arranged by size: 1524 1354 787 624 597 491 393 268 257 EcoO109I rG'GnC_Cy Cuts at: 2134 2307 2438 2964 2134 Size: 173 131 526 5465 Fragments arranged by size: 5465 526 173 131 EcoRI G'AATT_C Cuts at: 3604 3982 3604 Size: 378 5917 FspI TGC'GCA Cuts at: 4801 4801 Size: 6295 HaeII r_GCGC'y Cuts at: 1678 2029 2726 5442 1678 Size: 351 697 2716 2531 Fragments arranged by size: 2716 2531 697 351 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2629 2661 3062 3094 4936 Size: 32 135 32 1057 32 401 32 1842 Cuts at: 4936 4968 5010 5042 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1842 1057 401 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3639 3885 669 Size: 988 281 1701 246 3079 Fragments arranged by size: 3079 1701 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6295 HpaI GTT'AAC Cuts at: 3885 3885 Size: 6295 KpnI G_GTAC'C Cuts at: 3620 3620 Size: 6295 MfeI C'AATT_G Cuts at: 3894 3894 Size: 6295 MluI A'CGCG_T Cuts at: 6171 6171 Size: 6295 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5409 5593 1305 Size: 4104 184 2007 Fragments arranged by size: 4104 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4473 Fragments arranged by size: 4473 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2276 2345 2579 3086 3182 3467 4253 Size: 648 1110 69 234 507 96 285 786 Cuts at: 4253 4612 4771 518 Size: 359 159 2042 Fragments arranged by size: 2042 1110 786 648 507 359 285 234 159 96 69 MspA1I CmG'CkG Cuts at: 1606 2101 2360 2519 2958 2989 3009 3318 3442 Size: 495 259 159 439 31 20 309 124 Cuts at: 3442 3549 3711 4388 5536 5781 1606 Size: 107 162 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 495 439 309 259 245 162 159 124 107 31 20 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4804 Fragments arranged by size: 4804 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6295 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6295 NotI GC'GGCC_GC Cuts at: 3742 3742 Size: 6295 NspI r_CATG'y Cuts at: 1132 1229 2974 3076 3325 3481 3653 5198 1132 Size: 97 1745 102 249 156 172 1545 2229 Fragments arranged by size: 2229 1745 1545 249 172 156 102 97 PasI CC'CwG_GG Cuts at: 1348 2303 1348 Size: 955 5340 PciI A'CATG_T Cuts at: 3477 5194 3477 Size: 1717 4578 PflMI CCAn_nnn'nTGG Cuts at: 1108 3256 1108 Size: 2148 4147 PmeI GTTT'AAAC Cuts at: 3599 3599 Size: 6295 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4364 4396 5877 5909 4364 Size: 32 1481 32 4750 Fragments arranged by size: 4750 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2307 2307 Size: 6295 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6295 PsiI TTA'TAA Cuts at: 3865 3865 Size: 6295 PspOMI G'GGCC_C Cuts at: 2437 2437 Size: 6295 PspXI vC'TCGA_Gb Cuts at: 3125 3125 Size: 6295 PstI C_TGCA'G Cuts at: 830 2478 3647 830 Size: 1648 1169 3478 Fragments arranged by size: 3478 1648 1169 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6295 PvuII CAG'CTG Cuts at: 2101 2360 3009 3549 2101 Size: 259 649 540 4847 Fragments arranged by size: 4847 649 540 259 SacII CC_GC'GG Cuts at: 2990 2990 Size: 6295 SalI G'TCGA_C Cuts at: 1936 3637 1936 Size: 1701 4594 SapI GCTCTTCn'nnn_ Cuts at: 2486 2786 2486 Size: 300 5995 SbfI CC_TGCA'GG Cuts at: 3647 3647 Size: 6295 ScaI AGT'ACT Cuts at: 4543 6255 4543 Size: 1712 4583 SfcI C'TryA_G Cuts at: 826 1045 2474 3520 3643 4778 5459 5650 826 Size: 219 1429 1046 123 1135 681 191 1471 Fragments arranged by size: 1471 1429 1135 1046 681 219 191 123 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6295 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6295 SmaI CCC'GGG Cuts at: 1872 2064 3622 1872 Size: 192 1558 4545 Fragments arranged by size: 4545 1558 192 SmlI C'TyrA_G Cuts at: 820 1017 1943 2030 3125 4399 5300 5562 820 Size: 197 926 87 1095 1274 901 262 1553 Fragments arranged by size: 1553 1274 1095 926 901 262 197 87 SnaBI TAC'GTA Cuts at: 493 4086 493 Size: 3593 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6295 SphI G_CATG'C Cuts at: 1132 3325 3653 1132 Size: 2193 328 3774 Fragments arranged by size: 3774 2193 328 SspI AAT'ATT Cuts at: 5 52 4219 5 Size: 47 4167 2081 Fragments arranged by size: 4167 2081 47 StuI AGG'CCT Cuts at: 1517 2498 2826 3591 1517 Size: 981 328 765 4221 Fragments arranged by size: 4221 981 765 328 StyI C'CwwG_G Cuts at: 513 1065 2004 2433 2993 3734 513 Size: 552 939 429 560 741 3074 Fragments arranged by size: 3074 939 741 560 552 429 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2907 3263 4322 4339 4492 4677 5122 Size: 1255 1327 356 1059 17 153 185 445 Cuts at: 5122 325 Size: 1498 Fragments arranged by size: 1498 1327 1255 1059 445 356 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4541 6253 96 Size: 274 80 33 51 1443 2564 1712 138 Fragments arranged by size: 2564 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2720 2752 6255 6287 2720 Size: 32 3503 32 2728 Fragments arranged by size: 3503 2728 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2864 3469 1316 Size: 1548 605 4142 Fragments arranged by size: 4142 1548 605 XbaI T'CTAG_A Cuts at: 3631 3631 Size: 6295 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 3175 2011 Size: 1164 5131 XhoI C'TCGA_G Cuts at: 1943 3125 1943 Size: 1182 5113 XmaI C'CCGG_G Cuts at: 1870 2062 3620 1870 Size: 192 1558 4545 Fragments arranged by size: 4545 1558 192 XmnI GAAnn'nnTTC Cuts at: 4424 4424 Size: 6295 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2878 4103 276 Size: 53 83 186 936 1344 1225 2468 Fragments arranged by size: 2468 1344 1225 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PsrI RsrII SacI SanDI SexAI SfiI SfoI SrfI SwaI