(Linear) (Six-base) MAPSORT of: KIEE2614.seq Check: 6794 from: 1 to: 1590 KIEE2614.orf With 182 enzymes: SgfI * March 25, 2016 09:08 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1256 1590 Size: 1256 334 AatII G_ACGT'C Cuts at: 0 875 1590 Size: 875 715 AccI GT'mk_AC Cuts at: 0 1304 1590 Size: 1304 286 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 999 1590 Size: 999 591 AflIII A'CryG_T Cuts at: 0 461 1472 1590 Size: 461 1011 118 Fragments arranged by size: 1011 461 118 AleI CACnn'nnGTG Cuts at: 0 271 1590 Size: 271 1319 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1264 1564 1590 Size: 1264 300 26 Fragments arranged by size: 1264 300 26 AlwNI CAG_nnn'CTG Cuts at: 0 455 1590 Size: 455 1135 ApaI G_GGCC'C Cuts at: 0 436 1590 Size: 436 1154 ApaLI G'TGCA_C Cuts at: 0 847 1590 Size: 847 743 AvaI C'yCGr_G Cuts at: 0 57 482 1120 1590 Size: 57 425 638 470 Fragments arranged by size: 638 470 425 57 BanI G'GyrC_C Cuts at: 0 520 1240 1590 Size: 520 720 350 Fragments arranged by size: 720 520 350 BanII G_rGCy'C Cuts at: 0 7 35 436 1023 1429 1590 Size: 7 28 401 587 406 161 Fragments arranged by size: 587 406 401 161 28 7 BbsI GAAGACnn'nnnn_ Cuts at: 0 1269 1590 Size: 1269 321 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 648 682 750 784 1590 Size: 648 34 68 34 806 Fragments arranged by size: 806 648 68 34 34 BciVI GTATCCnnnnn_n' Cuts at: 0 156 1590 Size: 156 1434 BglI GCCn_nnn'nGGC Cuts at: 0 809 1590 Size: 809 781 Bme1580I G_kGCm'C Cuts at: 0 188 436 523 851 1243 1400 1590 Size: 188 248 87 328 392 157 190 Fragments arranged by size: 392 328 248 190 188 157 87 BmgBI CAC'GTC Cuts at: 0 273 462 1590 Size: 273 189 1128 Fragments arranged by size: 1128 273 189 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 118 742 1590 Size: 118 624 848 Fragments arranged by size: 848 624 118 Bpu10I CC'TnA_GC Cuts at: 0 119 1590 Size: 119 1471 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 46 1590 Size: 46 1544 BsaAI yAC'GTr Cuts at: 0 1041 1590 Size: 1041 549 BsaHI Gr'CG_yC Cuts at: 0 146 872 1590 Size: 146 726 718 Fragments arranged by size: 726 718 146 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 715 745 1057 1087 1590 Size: 715 30 312 30 503 Fragments arranged by size: 715 503 312 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 43 115 1590 Size: 43 72 1475 Fragments arranged by size: 1475 72 43 BseYI C'CCAG_C Cuts at: 0 34 150 888 930 1309 1488 1590 Size: 34 116 738 42 379 179 102 Fragments arranged by size: 738 379 179 116 102 42 34 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 379 522 565 727 1015 1494 1534 1590 Size: 379 143 43 162 288 479 40 56 Fragments arranged by size: 479 379 288 162 143 56 43 40 BsiEI CG_ry'CG Cuts at: 0 793 1590 Size: 793 797 BsiHKAI G_wGCw'C Cuts at: 0 622 776 851 887 1590 Size: 622 154 75 36 703 Fragments arranged by size: 703 622 154 75 36 Bsp1286I G_dGCh'C Cuts at: 0 7 35 188 436 523 622 776 851 Size: 7 28 153 248 87 99 154 75 Cuts at: 851 887 1023 1243 1400 1429 1590 Size: 36 136 220 157 29 161 Fragments arranged by size: 248 220 161 157 154 153 136 99 87 75 36 29 28 7 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 290 1256 1590 Size: 290 966 334 Fragments arranged by size: 966 334 290 BsrFI r'CCGG_y Cuts at: 0 946 1590 Size: 946 644 BssHII G'CGCG_C Cuts at: 0 681 1590 Size: 681 909 BssSI C'ACGA_G Cuts at: 0 937 1590 Size: 937 653 BstEII G'GTnAC_C Cuts at: 0 942 1590 Size: 942 648 BstXI CCAn_nnnn'nTGG Cuts at: 0 507 1407 1590 Size: 507 900 183 Fragments arranged by size: 900 507 183 BstYI r'GATC_y Cuts at: 0 1130 1208 1590 Size: 1130 78 382 Fragments arranged by size: 1130 382 78 Bsu36I CC'TnA_GG Cuts at: 0 133 1590 Size: 133 1457 BtgI C'CryG_G Cuts at: 0 8 524 982 1226 1499 1590 Size: 8 516 458 244 273 91 Fragments arranged by size: 516 458 273 244 91 8 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 232 1062 1590 Size: 232 830 528 Fragments arranged by size: 830 528 232 BtsI GCAGTG_nn' Cuts at: 0 1137 1357 1590 Size: 1137 220 233 Fragments arranged by size: 1137 233 220 DraIII CAC_nnn'GTG Cuts at: 0 942 1103 1590 Size: 942 161 487 Fragments arranged by size: 942 487 161 DrdI GACnn_nn'nnGTC Cuts at: 0 1491 1590 Size: 1491 99 EaeI y'GGCC_r Cuts at: 0 509 790 985 1590 Size: 509 281 195 605 Fragments arranged by size: 605 509 281 195 EagI C'GGCC_G Cuts at: 0 790 1590 Size: 790 800 EarI CTCTTCn'nnn_ Cuts at: 0 481 781 1294 1590 Size: 481 300 513 296 Fragments arranged by size: 513 481 300 296 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 124 1123 1485 1590 Size: 124 999 362 105 Fragments arranged by size: 999 362 124 105 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 118 742 999 1590 Size: 118 624 257 591 Fragments arranged by size: 624 591 257 118 EcoO109I rG'GnC_Cy Cuts at: 0 129 302 433 959 1590 Size: 129 173 131 526 631 Fragments arranged by size: 631 526 173 131 129 HaeII r_GCGC'y Cuts at: 0 24 721 1590 Size: 24 697 869 Fragments arranged by size: 869 697 24 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 624 656 1057 1089 1590 Size: 624 32 401 32 501 Fragments arranged by size: 624 501 401 32 32 MslI CAynn'nnrTG Cuts at: 0 271 340 574 1081 1177 1462 1590 Size: 271 69 234 507 96 285 128 Fragments arranged by size: 507 285 271 234 128 96 69 MspA1I CmG'CkG Cuts at: 0 96 355 514 953 984 1004 1313 1437 Size: 96 259 159 439 31 20 309 124 Cuts at: 1437 1544 1590 Size: 107 46 Fragments arranged by size: 439 309 259 159 124 107 96 46 31 20 NspI r_CATG'y Cuts at: 0 969 1071 1320 1476 1590 Size: 969 102 249 156 114 Fragments arranged by size: 969 249 156 114 102 PasI CC'CwG_GG Cuts at: 0 298 1590 Size: 298 1292 PciI A'CATG_T Cuts at: 0 1472 1590 Size: 1472 118 PflMI CCAn_nnn'nTGG Cuts at: 0 1251 1590 Size: 1251 339 PpuMI rG'GwC_Cy Cuts at: 0 302 1590 Size: 302 1288 PspOMI G'GGCC_C Cuts at: 0 432 1590 Size: 432 1158 PspXI vC'TCGA_Gb Cuts at: 0 1120 1590 Size: 1120 470 PstI C_TGCA'G Cuts at: 0 473 1590 Size: 473 1117 PvuII CAG'CTG Cuts at: 0 96 355 1004 1544 1590 Size: 96 259 649 540 46 Fragments arranged by size: 649 540 259 96 46 SacII CC_GC'GG Cuts at: 0 985 1590 Size: 985 605 SapI GCTCTTCn'nnn_ Cuts at: 0 481 781 1590 Size: 481 300 809 Fragments arranged by size: 809 481 300 SfcI C'TryA_G Cuts at: 0 469 1515 1590 Size: 469 1046 75 Fragments arranged by size: 1046 469 75 SmaI CCC'GGG Cuts at: 0 59 1590 Size: 59 1531 SmlI C'TyrA_G Cuts at: 0 25 1120 1590 Size: 25 1095 470 Fragments arranged by size: 1095 470 25 SphI G_CATG'C Cuts at: 0 1320 1590 Size: 1320 270 StuI AGG'CCT Cuts at: 0 493 821 1586 1590 Size: 493 328 765 4 Fragments arranged by size: 765 493 328 4 StyI C'CwwG_G Cuts at: 0 428 988 1590 Size: 428 560 602 Fragments arranged by size: 602 560 428 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 902 1258 1590 Size: 902 356 332 Fragments arranged by size: 902 356 332 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 715 747 1590 Size: 715 32 843 Fragments arranged by size: 843 715 32 Tth111I GACn'n_nGTC Cuts at: 0 859 1464 1590 Size: 859 605 126 Fragments arranged by size: 859 605 126 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1170 1590 Size: 1170 420 XhoI C'TCGA_G Cuts at: 0 1120 1590 Size: 1120 470 XmaI C'CCGG_G Cuts at: 0 57 1590 Size: 57 1533 ZraI GAC'GTC Cuts at: 0 873 1590 Size: 873 717 Enzymes that do cut: AarI AatII AccI AcuI AflIII AleI AlfI AlwNI ApaI ApaLI AvaI BanI BanII BbsI BcgI BciVI BglI Bme1580I BmgBI BpmI Bpu10I BpuEI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrFI BssHII BssSI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I HaeII Hin4I MslI MspA1I NspI PasI PciI PflMI PpuMI PspOMI PspXI PstI PvuII SacII SapI SfcI SmaI SmlI SphI StuI StyI TaqII TstI Tth111I XcmI XhoI XmaI ZraI Enzymes that do not cut: Acc65I AclI AfeI AflII AgeI AhdI AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BclI BfrBI BglII BlpI BmrI BmtI BplI BsaI BsaBI BsaWI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BstAPI BstBI BstZ17I ClaI CspCI DraI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PfoI PmeI PmlI PpiI PshAI PsiI PsrI PvuI RsrII SacI SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI TatI XbaI XmnI