Restriction map for
pFN21AE5262
(Circular) (Six-base) MAPSORT of: pFN21AE5262.seq Check: 7067 from: 1 to: 5242
pFN21AE5262 5242 bp
With 182 enzymes: SgfI *
January 30, 2014 17:16 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3052 278
Size: 53 83 186 936 1516 2468
Fragments arranged by size:
2468 1516 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2585 1937
Size: 648 4594
Acc65I G'GTAC_C
Cuts at: 2563 2563
Size: 5242
AclI AA'CG_TT
Cuts at: 1563 3369 3742 1563
Size: 1806 373 3063
Fragments arranged by size:
3063 1806 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2441 3305 4689 4957 1632
Size: 809 864 1384 268 1917
Fragments arranged by size:
1917 1384 864 809 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5045
AflIII A'CryG_T
Cuts at: 1203 2418 4141 5118 1203
Size: 1215 1723 977 1327
Fragments arranged by size:
1723 1327 1215 977
AgeI A'CCGG_T
Cuts at: 4073 4073
Size: 5242
AhdI GACnn_n'nnGTC
Cuts at: 3971 3971
Size: 5242
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5242
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5242
AlwNI CAG_nnn'CTG
Cuts at: 2439 2985 4557 2439
Size: 546 1572 3124
Fragments arranged by size:
3124 1572 546
ApaLI G'TGCA_C
Cuts at: 3299 4455 3299
Size: 1156 4086
ApoI r'AATT_y
Cuts at: 1493 2468 2551 2777 2929 5038 1493
Size: 975 83 226 152 2109 1697
Fragments arranged by size:
2109 1697 975 226 152 83
AseI AT'TA_AT
Cuts at: 160 1032 3796 160
Size: 872 2764 1606
Fragments arranged by size:
2764 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5242
AvaI C'yCGr_G
Cuts at: 1870 1943 2567 1870
Size: 73 624 4545
Fragments arranged by size:
4545 624 73
BamHI G'GATC_C
Cuts at: 2572 2939 2572
Size: 367 4875
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2078 2563 4018 618
Size: 325 217 627 291 485 1455 1842
Fragments arranged by size:
1842 1455 627 485 325 291 217
BanII G_rGCy'C
Cuts at: 1391 2313 2561 1391
Size: 922 248 4072
Fragments arranged by size:
4072 922 248
BbeI G_GCGC'C
Cuts at: 2082 2082
Size: 5242
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2320 928
Size: 971 421 3850
Fragments arranged by size:
3850 971 421
BbvCI CC'TCA_GC
Cuts at: 2022 2118 2022
Size: 96 5146
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3433 3467 1903
Size: 34 1496 34 3678
Fragments arranged by size:
3678 1496 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3134 4344 3134
Size: 1210 4032
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5242
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3853 136
Size: 107 122 71 631 605 2181 1525
Fragments arranged by size:
2181 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5237 5237
Size: 5242
BlpI GC'TnA_GC
Cuts at: 2659 2659
Size: 5242
Bme1580I G_kGCm'C
Cuts at: 1790 3303 4459 1790
Size: 1513 1156 2573
Fragments arranged by size:
2573 1513 1156
BmgBI CAC'GTC
Cuts at: 1318 2320 1318
Size: 1002 4240
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2973 3931 442
Size: 416 53 83 399 24 1556 958 1753
Fragments arranged by size:
1753 1556 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5242
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2068 3902 1239
Size: 829 1834 2579
Fragments arranged by size:
2579 1834 829
Bpu10I CC'TnA_GC
Cuts at: 2022 2118 2307 2022
Size: 96 189 4957
Fragments arranged by size:
4957 189 96
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3367 4232 4530 3367
Size: 865 298 4079
Fragments arranged by size:
4079 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2195 3905 882
Size: 617 696 1710 2219
Fragments arranged by size:
2219 1710 696 617
BsaAI yAC'GTr
Cuts at: 493 1204 3033 493
Size: 711 1829 2702
Fragments arranged by size:
2702 1829 711
BsaBI GATnn'nnATC
Cuts at: 5236 5236
Size: 5242
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2079 2259 3049 3431
Size: 53 83 186 936 546 180 790 382
Cuts at: 3431 4066 275
Size: 635 1451
Fragments arranged by size:
1451 936 790 635 546 382 186 180
83 53
BsaWI w'CCGG_w
Cuts at: 3675 4073 4347 4494 4905 3675
Size: 398 274 147 411 4012
Fragments arranged by size:
4012 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5017
Fragments arranged by size:
5017 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2252 1186
Size: 1066 4176
BseYI C'CCAG_C
Cuts at: 4445 4941 4445
Size: 496 4746
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2249 2317 1163
Size: 573 513 68 4088
Fragments arranged by size:
4088 573 513 68
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2164 2541 2692 3453 4481 1540
Size: 275 186 163 377 151 761 1028 2301
Fragments arranged by size:
2301 1028 761 377 275 186 163 151
BsiHKAI G_wGCw'C
Cuts at: 2313 3303 3388 4459 2313
Size: 990 85 1071 3096
Fragments arranged by size:
3096 1071 990 85
BsmI GAATG_Cn'
Cuts at: 2753 2846 2753
Size: 93 5149
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2313 2561 3303 3388 4459 1391
Size: 399 523 248 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 523 399 248 85
BspEI T'CCGG_A
Cuts at: 4905 4905
Size: 5242
BspHI T'CATG_A
Cuts at: 3131 4861 3131
Size: 1730 3512
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2597 844
Size: 1753 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2244 3129 1394
Size: 429 421 885 3507
Fragments arranged by size:
3507 885 429 421
BsrDI GCAATG_nn'
Cuts at: 65 1248 3737 3911 65
Size: 1183 2489 174 1396
Fragments arranged by size:
2489 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2159 2163 3886 4073 1700
Size: 110 349 4 1723 187 2869
Fragments arranged by size:
2869 1723 349 187 110 4
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5242
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5242
BssSI C'ACGA_G
Cuts at: 3302 4314 3302
Size: 1012 4230
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5242
BstBI TT'CG_AA
Cuts at: 4046 4046
Size: 5242
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5242
BstYI r'GATC_y
Cuts at: 1972 2403 2572 2939 3324 3341 4793 4879 4891
Size: 431 169 367 385 17 1452 86 12
Cuts at: 4891 5237 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 431 385 367 346 169 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2073 2358 2473 513
Size: 552 1008 285 115 3282
Fragments arranged by size:
3282 1008 552 285 115
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4503
Fragments arranged by size:
4503 683 56
BtsI GCAGTG_nn'
Cuts at: 2758 3552 3572 2758
Size: 794 20 4428
Fragments arranged by size:
4428 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5242
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5207
DraI TTT'AAA
Cuts at: 2546 2546
Size: 5242
DraIII CAC_nnn'GTG
Cuts at: 2272 2272
Size: 5242
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2324 4249 809
Size: 507 1008 1925 1802
Fragments arranged by size:
1925 1802 1008 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2161 2689 3578
Size: 54 1425 50 275 18 331 528 889
Cuts at: 3578 5207 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 528 331 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2161 2689 1537
Size: 275 349 528 4090
Fragments arranged by size:
4090 528 349 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3172 1353
Size: 1819 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3829 4203 4349 387
Size: 1064 138 2240 374 146 1280
Fragments arranged by size:
2240 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2311 2311
Size: 5242
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2068 2441 3305 3902 4689 4957 1239
Size: 393 436 373 864 597 787 268 1524
Fragments arranged by size:
1524 864 787 597 436 393 373 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2267 2267
Size: 5242
EcoO109I rG'GnC_Cy
Cuts at: 2352 2352
Size: 5242
EcoRI G'AATT_C
Cuts at: 2551 2929 2551
Size: 378 4864
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2167 2167
Size: 5242
FseI GG_CCGG'CC
Cuts at: 2163 2163
Size: 5242
FspI TGC'GCA
Cuts at: 3748 3748
Size: 5242
HaeII r_GCGC'y
Cuts at: 1678 2082 4389 1678
Size: 404 2307 2531
Fragments arranged by size:
2531 2307 404
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2190 2222 3883 3915 3957
Size: 32 135 32 618 32 1661 32 42
Cuts at: 3957 3989 1373
Size: 32 2626
Fragments arranged by size:
2626 1661 618 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2586 2832 669
Size: 988 281 648 246 3079
Fragments arranged by size:
3079 988 648 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5242
HpaI GTT'AAC
Cuts at: 2832 2832
Size: 5242
KasI G'GCGC_C
Cuts at: 2078 2078
Size: 5242
KpnI G_GTAC'C
Cuts at: 2567 2567
Size: 5242
MfeI C'AATT_G
Cuts at: 2841 2841
Size: 5242
MluI A'CGCG_T
Cuts at: 5118 5118
Size: 5242
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2030 4356 4540 1305
Size: 725 2326 184 2007
Fragments arranged by size:
2326 2007 725 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3420
Fragments arranged by size:
3420 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2318 3200 3559 3718 518
Size: 648 1152 882 359 159 2042
Fragments arranged by size:
2042 1152 882 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2395 2658 3335 4483 4728 1606
Size: 789 263 677 1148 245 2120
Fragments arranged by size:
2120 1148 789 677 263 245
NaeI GCC'GGC
Cuts at: 2161 2161
Size: 5242
NarI GG'CG_CC
Cuts at: 2079 2079
Size: 5242
NcoI C'CATG_G
Cuts at: 513 1065 2073 513
Size: 552 1008 3682
Fragments arranged by size:
3682 1008 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5242
NgoMIV G'CCGG_C
Cuts at: 2159 2159
Size: 5242
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5242
NotI GC'GGCC_GC
Cuts at: 2689 2689
Size: 5242
NspI r_CATG'y
Cuts at: 1132 1229 2327 2422 2600 4145 1132
Size: 97 1098 95 178 1545 2229
Fragments arranged by size:
2229 1545 1098 178 97 95
PasI CC'CwG_GG
Cuts at: 1348 2066 1348
Size: 718 4524
PciI A'CATG_T
Cuts at: 2418 4141 2418
Size: 1723 3519
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5242
PfoI T'CCnGG_A
Cuts at: 2508 2508
Size: 5242
PmeI GTTT'AAAC
Cuts at: 2546 2546
Size: 5242
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3311 3343 4824 4856 3311
Size: 32 1481 32 3697
Fragments arranged by size:
3697 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2352 2352
Size: 5242
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5242
PsiI TTA'TAA
Cuts at: 2812 2812
Size: 5242
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2471 2503 2471
Size: 32 5210
PstI C_TGCA'G
Cuts at: 830 2115 2235 2594 830
Size: 1285 120 359 3478
Fragments arranged by size:
3478 1285 359 120
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5242
PvuII CAG'CTG
Cuts at: 2395 2395
Size: 5242
SacI G_AGCT'C
Cuts at: 2313 2313
Size: 5242
SalI G'TCGA_C
Cuts at: 1936 2584 1936
Size: 648 4594
SanDI GG'GwC_CC
Cuts at: 2352 2352
Size: 5242
SbfI CC_TGCA'GG
Cuts at: 2594 2594
Size: 5242
ScaI AGT'ACT
Cuts at: 3490 5202 3490
Size: 1712 3530
SfcI C'TryA_G
Cuts at: 826 1045 2111 2231 2590 3725 4406 4597 826
Size: 219 1066 120 359 1135 681 191 1471
Fragments arranged by size:
1471 1135 1066 681 359 219 191 120
SfoI GGC'GCC
Cuts at: 2080 2080
Size: 5242
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5242
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5242
SmaI CCC'GGG
Cuts at: 1872 2569 1872
Size: 697 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3346 4247 4509 820
Size: 197 926 1403 901 262 1553
Fragments arranged by size:
1553 1403 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3033 493
Size: 2540 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5242
SphI G_CATG'C
Cuts at: 1132 2600 1132
Size: 1468 3774
SspI AAT'ATT
Cuts at: 5 52 3166 5
Size: 47 3114 2081
Fragments arranged by size:
3114 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5242
StyI C'CwwG_G
Cuts at: 513 1065 2073 2681 513
Size: 552 1008 608 3074
Fragments arranged by size:
3074 1008 608 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3269 3286 3439 3624 4069 325
Size: 1255 1689 17 153 185 445 1498
Fragments arranged by size:
1689 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3488 5200 96
Size: 274 80 33 51 1443 1511 1712 138
Fragments arranged by size:
1712 1511 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5202 5234 5202
Size: 32 5210
Tth111I GACn'n_nGTC
Cuts at: 1316 2537 1316
Size: 1221 4021
XbaI T'CTAG_A
Cuts at: 2578 2578
Size: 5242
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2046 2046
Size: 5242
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5242
XmaI C'CCGG_G
Cuts at: 1870 2567 1870
Size: 697 4545
XmnI GAAnn'nnTTC
Cuts at: 2468 3371 2468
Size: 903 4339
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3050 276
Size: 53 83 186 936 1516 2468
Fragments arranged by size:
2468 1516 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FseI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PsrI PstI PvuI PvuII SacI SalI SanDI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV FspAI
NruI NsiI PacI PmlI PspOMI PspXI RsrII SacII
SapI SexAI SfiI SrfI SwaI