Restriction map for
pFN21AE6531
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# NO CUT ENZYMES ( 166 enzymes) #
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AatII AccI AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI AspEI AspHI
AspI AvaI AvaII AviII BamHI
BbeI BbrPI BbsI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlpI BpmI Bpu1102I BpuAI BsaBI
BsaI BseAI BseRI BsgI BsiEI
BsiHKAI BsiWI BsmAI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII CfoI Cfr10I Cfr13I
ClaI CpoI DraI DraII DraIII
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HhaI HinP1I
HincII HindII HindIII HpaI KasI
KspI MamI MluI MroI MslI
MvnI MwoI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI Sau96I SbfI ScaI
SexAI SfaNI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SwaI
Tth111I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 64 enzymes) #
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Acc65I 422 GGTACC G^GTACC
AciI 393 CCGC CCGC(-3/-1)
AcyI 75 GACGCC G[GA]^CG[CT]C
AfaI 423 GTAC GT^AC
AlwI 500 GGATC GGATC(4/5)
Asp700I 276 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 422 GGTACC G^GTACC
AvrII 79 CCTAGG C^CTAGG
BalI 471 TGGCCA TGG^CCA
BanI 422 GGTACC G^G[CT][GA]CC
BanII 30 GAGCCC G[GA]GC[CT]^C
BbvCI 58 CCTCAGC CCTCAGC(-5/-2)
BbvI 532 GCAGC GCAGC(8/12)
BlnI 79 CCTAGG C^CTAGG
BsaAI 556 TACGTG [CT]AC^GT[GA]
BsaHI 75 GACGCC G[GA]^CG[CT]C
BsaWI 69 TCCGGT [AT]^CCGG[AT]
BsrDI 510 CATTGC GCAATG(2/0)
BssKI 231 CCTGG ^CC[ACGT]GG
BstF5I 36 CATCC GGATG(2/0)
BstNI 231 CCTGG CC^[AT]GG
BstYI 500 GGATCT [GA]^GATC[CT]
DpnII 501 GATC ^GATC
DrdI 520 GACAGCTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 471 TGGCCA [CT]^GGCC[GA]
EarI 222 GAAGAG CTCTTC(1/4)
EcoRII 231 CCTGG ^CC[AT]GG
EcoT14I 79 CCTAGG C^C[AT][AT]GG
Fnu4HI 532 GCAGC GC^[ACGT]GC
FokI 36 CATCC GGATG(9/13)
HapII 70 CCGG C^CGG
HgaI 75 GACGC GACGC(5/10)
Hin1I 75 GACGCC G[GA]^CG[CT]C
HpaII 70 CCGG C^CGG
HphI 72 GGTGA GGTGA(8/7)
ItaI 532 GCAGC GC^[ACGT]GC
KpnI 422 GGTACC GGTAC^C
Ksp632I 222 GAAGAG CTCTTC(1/4)
MaeII 557 ACGT A^CGT
MboI 501 GATC ^GATC
MfeI 197 CAATTG C^AATTG
MflI 500 GGATCT [GA]^GATC[CT]
MluNI 471 TGGCCA TGG^CCA
MscI 471 TGGCCA TGG^CCA
MspA1I 522 CAGCTG C[AC]G^C[GT]G
MspI 70 CCGG C^CGG
MunI 197 CAATTG C^AATTG
MvaI 231 CCTGG CC^[AT]GG
NdeII 501 GATC ^GATC
NspI 101 GCATGT [GA]CATG^[CT]
PflMI 491 CCAGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 522 CAGCTG CAG^CTG
RsaI 423 GTAC GT^AC
Sau3AI 501 GATC ^GATC
ScrFI 231 CCTGG CC^[ACGT]GG
SspI 484 AATATT AAT^ATT
StuI 94 AGGCCT AGG^CCT
StyI 79 CCTAGG C^C[AT][AT]GG
TfiI 550 GAATC G^A[AT]TC
TseI 532 GCAGC G^C[AT]GC
TspRI 563 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 491 CCAGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 500 GGATCT [GA]^GATC[CT]
XmnI 276 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcsI 280 AAATTC [GA]^AATT[CT]
415 GAATTT
ApoI 280 AAATTC [GA]^AATT[CT]
415 GAATTT
BfaI 80 CTAG C^TAG
98 CTAG
BmyI 30 GAGCCC G[AGT]GC[ACT]^C
227 GTGCCC
BsiYI 57 CCCTCAGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
491 CCAGCAGATGG
BslI 57 CCCTCAGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
491 CCAGCAGATGG
BsmFI 506 GGGAC GGGAC(10/14)
518 GGGAC
Bsp1286I 30 GAGCCC G[AGT]GC[ACT]^C
227 GTGCCC
BsrI 469 ACTGG ACTGG(1/-1)
564 ACTGG
Cac8I 63 GCAGGC GC[ACGT]^[ACGT]GC
490 GCCAGC
DdeI 59 CTCAG C^T[ACGT]AG
145 CTGAG
MaeI 80 CTAG C^TAG
98 CTAG
MaeIII 73 GTGAC ^GT[ACGT]AC
375 GTCAC
MboII 222 GAAGA GAAGA(8/7)
276 GAAGA
NlaIII 102 CATG CATG^
515 CATG
PleI 193 GACTC GAGTC(4/5)
151 GAGTC
TaqI 28 TCGA T^CGA
107 TCGA
Tsp45I 73 GTGAC ^GT[GC]AC
375 GTCAC
TthHB8I 28 TCGA T^CGA
107 TCGA
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# 3 OR MORE CUT ENZYMES ( 9 enzymes) #
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AluI BsaJI HaeIII HinfI MnlI
MseI NlaIV Tru9I Tsp509I