################################## # NO CUT ENZYMES ( 162 enzymes) # ################################## AatII Acc65I AccI AclI AcyI AflII AflIII AgeI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspHI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BseRI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraIII DrdI DsaI EaeI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI HphI KasI KpnI KspI MaeI MamI MluI MluNI MscI MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AccII 19 CGCG CG^CG AccIII 174 TCCGGA T^CCGGA AcsI 149 AAATTC [GA]^AATT[CT] AhdI 582 GACAATTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 261 GGATC GGATC(4/5) AlwNI 622 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 23 GGGCCC GGGCC^C ApoI 149 AAATTC [GA]^AATT[CT] AspEI 582 GACAATTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BanII 23 GGGCCC G[GA]GC[CT]^C BbsI 736 GTCTTC GAAGAC(2/6) BmyI 23 GGGCCC G[AGT]GC[ACT]^C BpuAI 736 GTCTTC GAAGAC(2/6) BsaI 167 GAGACC GGTCTC(1/5) BsaWI 174 TCCGGA [AT]^CCGG[AT] BseAI 174 TCCGGA T^CCGGA BsgI 636 CTGCAC GTGCAG(16/14) Bsp1286I 23 GGGCCC G[AGT]GC[ACT]^C BspEI 174 TCCGGA T^CCGGA BspMI 358 ACCTGC ACCTGC(4/8) BstUI 19 CGCG CG^CG BstYI 261 GGATCT [GA]^GATC[CT] DraI 502 TTTAAA TTT^AAA Eam1105I 582 GACAATTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 250 GAAGAG CTCTTC(1/4) FauI 20 GCGGG CCCGC(4/6) HaeII 77 AGCGCC [GA]GCGC^[CT] HinfI 716 GAGTC G^A[ACGT]TC Ksp632I 250 GAAGAG CTCTTC(1/4) MaeII 296 ACGT A^CGT MaeIII 86 GTGAC ^GT[ACGT]AC MfeI 584 CAATTG C^AATTG MflI 261 GGATCT [GA]^GATC[CT] MroI 174 TCCGGA T^CCGGA MspA1I 625 CAGCTG C[AC]G^C[GT]G MunI 584 CAATTG C^AATTG MvnI 19 CGCG CG^CG PleI 716 GAGTC GAGTC(4/5) PpuMI 355 GGGACCT [GA]G^G[AT]CC[CT] PstI 8 CTGCAG CTGCA^G PvuII 625 CAGCTG CAG^CTG ScaI 398 AGTACT AGT^ACT Tsp45I 86 GTGAC ^GT[GC]AC XhoII 261 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AfaI 399 GTAC GT^AC 577 GTAC AluI 431 AGCT AG^CT 626 AGCT AvaII 356 GGACC G^G[AT]CC 435 GGACC BcnI 646 CCGGG CC^[GC]GG 705 CCGGG BsmAI 133 GAGAC GTCTC(1/5) 167 GAGAC BsmFI 355 GGGAC GGGAC(10/14) 660 GTCCC Cac8I 80 GCCTGC GC[ACGT]^[ACGT]GC 681 GCTGGC DpnII 230 GATC ^GATC 262 GATC MboI 230 GATC ^GATC 262 GATC MseI 257 TTAA T^TAA 503 TTAA NciI 646 CCGGG CC^[GC]GG 705 CCGGG NdeII 230 GATC ^GATC 262 GATC RsaI 399 GTAC GT^AC 577 GTAC Sau3AI 230 GATC ^GATC 262 GATC SfaNI 376 GATGC GCATC(5/9) 508 GATGC Tru9I 257 TTAA T^TAA 503 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 36 enzymes) # ######################################### AciI BbvI BsaJI BsiYI BslI BsrI BssKI BstF5I BstNI CfoI Cfr13I DdeI DraII EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII ItaI MboII MnlI MspI MvaI MwoI NlaIII NlaIV Sau96I ScrFI SfcI TseI Tsp509I TspRI