(Linear) (Six-base) MAPSORT of: KIEE5283.seq  Check: 1849  from: 1  to: 768



KIEE5283.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:54  ..

AhdI GACnn_n'nnGTC

Cuts at:      0     587     768 
   Size:        587     181
 
AlwNI CAG_nnn'CTG

Cuts at:      0     627     768 
   Size:        627     141
 
ApaI G_GGCC'C

Cuts at:      0      27     768 
   Size:         27     741
 
ApoI r'AATT_y

Cuts at:      0     149     768 
   Size:        149     619
 
BanII G_rGCy'C

Cuts at:      0      27     768 
   Size:         27     741
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     729     768 
   Size:        729      39
 
Bme1580I G_kGCm'C

Cuts at:      0      27     768 
   Size:         27     741
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     727     768 
   Size:        727      41
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     161     768 
   Size:        161     607
 
BsaWI w'CCGG_w

Cuts at:      0     174     768 
   Size:        174     594
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     643     673     768 
   Size:        643      30      95

  Fragments arranged by size: 

                 643      95      30
 
BseYI C'CCAG_C

Cuts at:      0      30     768 
   Size:         30     738
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     621     768 
   Size:        621     147
 
Bsp1286I G_dGCh'C

Cuts at:      0      27     768 
   Size:         27     741
 
BspEI T'CCGG_A

Cuts at:      0     174     768 
   Size:        174     594
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     367     768 
   Size:        367     401
 
BstYI r'GATC_y

Cuts at:      0     261     768 
   Size:        261     507
 
DraI TTT'AAA

Cuts at:      0     504     768 
   Size:        504     264
 
EarI CTCTTCn'nnn_

Cuts at:      0     245     768 
   Size:        245     523
 
EcoO109I rG'GnC_Cy

Cuts at:      0      23      24     356     768 
   Size:         23       1     332     412

  Fragments arranged by size: 

                 412     332      23       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     417     529     768 
   Size:        417     112     239

  Fragments arranged by size: 

                 417     239     112
 
HaeII r_GCGC'y

Cuts at:      0      81     768 
   Size:         81     687
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     246     278     573     605     643     675     693     725 
   Size:        246      32     295      32      38      32      18      32

Cuts at:    725     768 
   Size:         43

  Fragments arranged by size: 

                 295     246      43      38      32      32      32      32
                  18
 
MfeI C'AATT_G

Cuts at:      0     584     768 
   Size:        584     184
 
MspA1I CmG'CkG

Cuts at:      0     627     768 
   Size:        627     141
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     590     622     768 
   Size:        590      32     146

  Fragments arranged by size: 

                 590     146      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     356     768 
   Size:        356     412
 
PspOMI G'GGCC_C

Cuts at:      0      23     768 
   Size:         23     745
 
PstI C_TGCA'G

Cuts at:      0      12     768 
   Size:         12     756
 
PvuII CAG'CTG

Cuts at:      0     627     768 
   Size:        627     141
 
ScaI AGT'ACT

Cuts at:      0     400     768 
   Size:        400     368
 
SfcI C'TryA_G

Cuts at:      0       8     162     423     768 
   Size:          8     154     261     345

  Fragments arranged by size: 

                 345     261     154       8
 
TatI w'GTAC_w

Cuts at:      0     398     576     768 
   Size:        398     178     192

  Fragments arranged by size: 

                 398     192     178
 

 Enzymes that do cut:

     AhdI    AlwNI     ApaI     ApoI    BanII     BbsI Bme1580I     BmrI
     BsaI    BsaWI    BsaXI    BseYI     BsgI Bsp1286I    BspEI    BspMI
    BstYI     DraI     EarI EcoO109I     FalI    HaeII    Hin4I     MfeI
   MspA1I     PpiI    PpuMI   PspOMI     PstI    PvuII     ScaI     SfcI
     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AleI     AlfI     AloI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI
     BmtI     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI
    BseRI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspHI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI  Eco57MI    EcoNI
    EcoRI    EcoRV     FseI     FspI    FspAI   HincII  HindIII     HpaI
     KasI     KpnI     MluI     MmeI     MscI     MslI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI    PshAI
     PsiI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     StyI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI