(Circular) (Six-base) MAPSORT of: pFN21AE1824.seq Check: 2775 from: 1 to: 5728 pFN21AE1824 5728 bp With 182 enzymes: SgfI * June 20, 2019 11:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3538 278 Size: 53 83 186 936 2002 2468 Fragments arranged by size: 2468 2002 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3071 1937 Size: 1134 4594 Acc65I G'GTAC_C Cuts at: 2542 3049 2542 Size: 507 5221 AclI AA'CG_TT Cuts at: 1563 3027 3855 4228 1563 Size: 1464 828 373 3063 Fragments arranged by size: 3063 1464 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2591 3791 5175 5443 1632 Size: 959 1200 1384 268 1917 Fragments arranged by size: 1917 1384 1200 959 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5531 AflIII A'CryG_T Cuts at: 1203 2169 2458 4627 5604 1203 Size: 966 289 2169 977 1327 Fragments arranged by size: 2169 1327 977 966 289 AgeI A'CCGG_T Cuts at: 4559 4559 Size: 5728 AhdI GACnn_n'nnGTC Cuts at: 4457 4457 Size: 5728 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5728 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5728 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2226 2258 2226 Size: 32 5696 AlwNI CAG_nnn'CTG Cuts at: 3471 5043 3471 Size: 1572 4156 ApaI G_GGCC'C Cuts at: 2841 2841 Size: 5728 ApaLI G'TGCA_C Cuts at: 3785 4941 3785 Size: 1156 4572 ApoI r'AATT_y Cuts at: 1493 3037 3263 3415 5524 1493 Size: 1544 226 152 2109 1697 Fragments arranged by size: 2109 1697 1544 226 152 AseI AT'TA_AT Cuts at: 160 1032 4282 160 Size: 872 3250 1606 Fragments arranged by size: 3250 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5728 AvaI C'yCGr_G Cuts at: 1870 1943 2126 3053 1870 Size: 73 183 927 4545 Fragments arranged by size: 4545 927 183 73 BamHI G'GATC_C Cuts at: 3058 3425 3058 Size: 367 5361 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2542 2609 2887 3049 4504 Size: 325 217 627 755 67 278 162 1455 Cuts at: 4504 618 Size: 1842 Fragments arranged by size: 1842 1455 755 627 325 278 217 162 67 BanII G_rGCy'C Cuts at: 1391 2841 3047 1391 Size: 1450 206 4072 Fragments arranged by size: 4072 1450 206 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2568 928 Size: 971 669 4088 Fragments arranged by size: 4088 971 669 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3919 3953 1903 Size: 34 1982 34 3678 Fragments arranged by size: 3678 1982 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2922 3620 4830 2922 Size: 698 1210 3820 Fragments arranged by size: 3820 1210 698 BclI T'GATC_A Cuts at: 1548 2766 1548 Size: 1218 4510 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4339 136 Size: 107 122 71 631 605 2667 1525 Fragments arranged by size: 2667 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5723 5723 Size: 5728 BlpI GC'TnA_GC Cuts at: 3145 3145 Size: 5728 Bme1580I G_kGCm'C Cuts at: 1790 2841 3789 4945 1790 Size: 1051 948 1156 2573 Fragments arranged by size: 2573 1156 1051 948 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5728 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2703 3459 4417 Size: 416 53 83 399 24 1286 756 958 Cuts at: 4417 442 Size: 1753 Fragments arranged by size: 1753 1286 958 756 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5728 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2817 4388 1239 Size: 1578 1571 2579 Fragments arranged by size: 2579 1578 1571 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2927 3853 4718 5016 2927 Size: 926 865 298 3639 Fragments arranged by size: 3639 926 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4391 882 Size: 617 2892 2219 Fragments arranged by size: 2892 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3519 493 Size: 711 2315 2702 Fragments arranged by size: 2702 2315 711 BsaBI GATnn'nnATC Cuts at: 5722 5722 Size: 5728 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3535 3917 4552 275 Size: 53 83 186 936 2002 382 635 1451 Fragments arranged by size: 2002 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2354 4161 4559 4833 4980 5391 2354 Size: 1807 398 274 147 411 2691 Fragments arranged by size: 2691 1807 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5503 Fragments arranged by size: 5503 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2423 2796 1186 Size: 1237 373 4118 Fragments arranged by size: 4118 1237 373 BseYI C'CCAG_C Cuts at: 4931 5427 4931 Size: 496 5232 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2232 1163 Size: 573 496 4659 Fragments arranged by size: 4659 573 496 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3178 3939 4967 1540 Size: 275 186 1177 761 1028 2301 Fragments arranged by size: 2301 1177 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3789 3874 4945 3789 Size: 85 1071 4572 Fragments arranged by size: 4572 1071 85 BsmI GAATG_Cn' Cuts at: 3239 3332 3239 Size: 93 5635 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2841 3047 3789 3874 4945 1391 Size: 399 1051 206 742 85 1071 2174 Fragments arranged by size: 2174 1071 1051 742 399 206 85 BspEI T'CCGG_A Cuts at: 5391 5391 Size: 5728 BspHI T'CATG_A Cuts at: 2085 3617 5347 2085 Size: 1532 1730 2466 Fragments arranged by size: 2466 1730 1532 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2554 3083 844 Size: 1710 529 3489 Fragments arranged by size: 3489 1710 529 BsrBI CCG'CTC Cuts at: 1394 1823 2131 3615 1394 Size: 429 308 1484 3507 Fragments arranged by size: 3507 1484 429 308 BsrDI GCAATG_nn' Cuts at: 65 1248 4223 4397 65 Size: 1183 2975 174 1396 Fragments arranged by size: 2975 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2704 3020 4372 4559 1700 Size: 110 894 316 1352 187 2869 Fragments arranged by size: 2869 1352 894 316 187 110 BsrGI T'GTAC_A Cuts at: 96 2172 96 Size: 2076 3652 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5728 BssSI C'ACGA_G Cuts at: 3788 4800 3788 Size: 1012 4716 BstAPI GCAn_nnn'nTGC Cuts at: 794 2971 794 Size: 2177 3551 BstBI TT'CG_AA Cuts at: 2959 4532 2959 Size: 1573 4155 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5728 BstYI r'GATC_y Cuts at: 1972 2112 3058 3425 3810 3827 5279 5365 5377 Size: 140 946 367 385 17 1452 86 12 Cuts at: 5377 5723 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 946 385 367 346 140 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4237 Fragments arranged by size: 4237 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4989 Fragments arranged by size: 4989 683 56 BtsI GCAGTG_nn' Cuts at: 3244 4038 4058 3244 Size: 794 20 4914 Fragments arranged by size: 4914 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5728 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5693 DraI TTT'AAA Cuts at: 3032 3032 Size: 5728 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4735 809 Size: 507 3419 1802 Fragments arranged by size: 3419 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3175 4064 5693 Size: 54 1425 50 275 18 1345 889 1629 Cuts at: 5693 8 Size: 43 Fragments arranged by size: 1629 1425 1345 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3175 1537 Size: 275 1363 4090 Fragments arranged by size: 4090 1363 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2504 3658 1353 Size: 1151 1154 3423 Fragments arranged by size: 3423 1154 1151 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4315 4689 4835 387 Size: 1064 138 2726 374 146 1280 Fragments arranged by size: 2726 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2591 2817 3791 4388 5175 5443 1239 Size: 393 959 226 974 597 787 268 1524 Fragments arranged by size: 1524 974 959 787 597 393 268 226 EcoO109I rG'GnC_Cy Cuts at: 2234 2837 2234 Size: 603 5125 EcoRI G'AATT_C Cuts at: 3037 3415 3037 Size: 378 5350 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2276 2276 Size: 5728 FspI TGC'GCA Cuts at: 4234 4234 Size: 5728 HaeII r_GCGC'y Cuts at: 1678 4875 1678 Size: 3197 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2349 2381 2484 2516 4369 Size: 32 135 32 777 32 103 32 1853 Cuts at: 4369 4401 4443 4475 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1853 777 135 103 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3072 3318 669 Size: 988 281 1134 246 3079 Fragments arranged by size: 3079 1134 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5728 HpaI GTT'AAC Cuts at: 3318 3318 Size: 5728 KpnI G_GTAC'C Cuts at: 2546 3053 2546 Size: 507 5221 MfeI C'AATT_G Cuts at: 2431 3327 2431 Size: 896 4832 MluI A'CGCG_T Cuts at: 5604 5604 Size: 5728 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4842 5026 1305 Size: 3537 184 2007 Fragments arranged by size: 3537 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3906 Fragments arranged by size: 3906 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2445 3686 4045 4204 518 Size: 648 1279 1241 359 159 2042 Fragments arranged by size: 2042 1279 1241 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2065 2584 2720 2744 2786 3144 3821 4969 Size: 459 519 136 24 42 358 677 1148 Cuts at: 4969 5214 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 519 459 358 245 136 42 24 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4237 Fragments arranged by size: 4237 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5728 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5728 NotI GC'GGCC_GC Cuts at: 3175 3175 Size: 5728 NspI r_CATG'y Cuts at: 1132 1229 2173 2462 3086 4631 1132 Size: 97 944 289 624 1545 2229 Fragments arranged by size: 2229 1545 944 624 289 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5728 PciI A'CATG_T Cuts at: 2169 2458 4627 2169 Size: 289 2169 3270 Fragments arranged by size: 3270 2169 289 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5728 PmeI GTTT'AAAC Cuts at: 3032 3032 Size: 5728 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3797 3829 5310 5342 3797 Size: 32 1481 32 4183 Fragments arranged by size: 4183 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2234 2234 Size: 5728 PshAI GACnn'nnGTC Cuts at: 1613 2786 1613 Size: 1173 4555 PsiI TTA'TAA Cuts at: 3298 3298 Size: 5728 PspOMI G'GGCC_C Cuts at: 2837 2837 Size: 5728 PspXI vC'TCGA_Gb Cuts at: 2126 2126 Size: 5728 PstI C_TGCA'G Cuts at: 830 2551 3080 830 Size: 1721 529 3478 Fragments arranged by size: 3478 1721 529 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5728 PvuII CAG'CTG Cuts at: 2065 2584 2720 2744 2065 Size: 519 136 24 5049 Fragments arranged by size: 5049 519 136 24 SalI G'TCGA_C Cuts at: 1936 3070 1936 Size: 1134 4594 SbfI CC_TGCA'GG Cuts at: 3080 3080 Size: 5728 ScaI AGT'ACT Cuts at: 3976 5688 3976 Size: 1712 4016 SfcI C'TryA_G Cuts at: 826 1045 2547 2614 3076 4211 4892 5083 826 Size: 219 1502 67 462 1135 681 191 1471 Fragments arranged by size: 1502 1471 1135 681 462 219 191 67 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5728 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5728 SmaI CCC'GGG Cuts at: 1872 3055 1872 Size: 1183 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2126 2906 3832 4733 4995 820 Size: 197 926 183 780 926 901 262 1553 Fragments arranged by size: 1553 926 926 901 780 262 197 183 SnaBI TAC'GTA Cuts at: 493 3519 493 Size: 3026 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5728 SphI G_CATG'C Cuts at: 1132 3086 1132 Size: 1954 3774 SspI AAT'ATT Cuts at: 5 52 3652 5 Size: 47 3600 2081 Fragments arranged by size: 3600 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5728 StyI C'CwwG_G Cuts at: 513 1065 2004 2311 2681 3167 513 Size: 552 939 307 370 486 3074 Fragments arranged by size: 3074 939 552 486 370 307 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2826 3024 3755 3772 3925 4110 4555 Size: 1255 1246 198 731 17 153 185 445 Cuts at: 4555 325 Size: 1498 Fragments arranged by size: 1498 1255 1246 731 445 198 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2172 2195 2461 Size: 274 80 33 51 1443 195 23 266 Cuts at: 2461 3974 5686 96 Size: 1513 1712 138 Fragments arranged by size: 1712 1513 1443 274 266 195 138 80 51 33 23 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5688 5720 5688 Size: 32 5696 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5728 XbaI T'CTAG_A Cuts at: 3064 3064 Size: 5728 XhoI C'TCGA_G Cuts at: 1943 2126 1943 Size: 183 5545 XmaI C'CCGG_G Cuts at: 1870 3053 1870 Size: 1183 4545 XmnI GAAnn'nnTTC Cuts at: 3857 3857 Size: 5728 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3536 276 Size: 53 83 186 936 2002 2468 Fragments arranged by size: 2468 2002 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI