################################## # NO CUT ENZYMES ( 149 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcyI AflII AflIII AgeI AhdI AocI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaHI BsgI BsiEI BsiWI BsmI BsoBI Bsp1407I BspDI BspHI BspLU11I BspMI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstF5I BstPI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FokI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MaeI MluI MluNI MscI MslI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AccIII 619 TCCGGA T^CCGGA AciI 82 CCGC CCGC(-3/-1) AcsI 220 AAATTC [GA]^AATT[CT] AfaI 735 GTAC GT^AC Alw44I 91 GTGCAC G^TGCAC AlwNI 517 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 698 AGCGCT AGC^GCT ApaLI 91 GTGCAC G^TGCAC ApoI 220 AAATTC [GA]^AATT[CT] AspHI 91 GTGCAC G[AT]GC[AT]^C BanI 558 GGTGCC G^G[CT][GA]CC BcnI 87 CCCGG CC^[GC]GG BmyI 91 GTGCAC G[AGT]GC[ACT]^C BsaAI 736 TACGTG [CT]AC^GT[GA] BsaBI 310 GATTACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 16 GAGACC GGTCTC(1/5) BsaJI 554 CCTTGG C^C[ACGT][ACGT]GG BsaWI 619 TCCGGA [AT]^CCGG[AT] BseAI 619 TCCGGA T^CCGGA BseRI 707 GAGGAG GAGGAG(10/8) BsiHKAI 91 GTGCAC G[AT]GC[AT]^C BsmBI 623 GAGACG CGTCTC(1/5) Bsp1286I 91 GTGCAC G[AGT]GC[ACT]^C BspEI 619 TCCGGA T^CCGGA BsrBI 82 CCGCTC CCGCTC(-3/-3) BsrI 684 ACTGG ACTGG(1/-1) BstXI 62 CCAAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 422 AGATCC [GA]^GATC[CT] CfoI 699 GCGC GCG^C DraI 184 TTTAAA TTT^AAA Eco47III 698 AGCGCT AGC^GCT EcoT14I 554 CCTTGG C^C[AT][AT]GG HaeII 698 AGCGCT [GA]GCGC^[CT] HhaI 699 GCGC GCG^C HinP1I 699 GCGC G^CGC HphI 235 GGTGA GGTGA(8/7) MaeII 737 ACGT A^CGT MamI 310 GATTACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 245 CAATTG C^AATTG MflI 422 AGATCC [GA]^GATC[CT] MroI 619 TCCGGA T^CCGGA MunI 245 CAATTG C^AATTG NciI 87 CCCGG CC^[GC]GG NspI 398 ACATGC [GA]CATG^[CT] PflMI 516 CCAGCATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 754 GAGTC GAGTC(4/5) PpuMI 509 AGGTCCT [GA]G^G[AT]CC[CT] PstI 173 CTGCAG CTGCA^G RsaI 735 GTAC GT^AC StyI 554 CCTTGG C^C[AT][AT]GG TspRI 683 CACTG CA[GC]TG[ACGT][ACGT]^ Van91I 516 CCAGCATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 422 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AlwI 423 GATCC GGATC(4/5) 672 GATCC BbvI 66 GCTGC GCAGC(8/12) 172 GCTGC BsiYI 169 CCAGCTGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 516 CCAGCATCTGG BslI 169 CCAGCTGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 516 CCAGCATCTGG BsmFI 665 GGGAC GGGAC(10/14) 742 GTCCC BstBI 215 TTCGAA TT^CGAA 264 TTCGAA BstNI 284 CCTGG CC^[AT]GG 675 CCAGG DpnII 423 GATC ^GATC 672 GATC DraII 162 AGGCCCT [GA]G^G[ACGT]CC[CT] 509 AGGTCCT EarI 54 GAAGAG CTCTTC(1/4) 648 GAAGAG EcoO109I 162 AGGCCCT [GA]G^G[ACGT]CC[CT] 509 AGGTCCT EcoRII 284 CCTGG ^CC[AT]GG 675 CCAGG HapII 88 CCGG C^CGG 620 CCGG HpaII 88 CCGG C^CGG 620 CCGG Ksp632I 54 GAAGAG CTCTTC(1/4) 648 GAAGAG MaeIII 236 GTGAC ^GT[ACGT]AC 499 GTCAC MboI 423 GATC ^GATC 672 GATC MboII 54 GAAGA GAAGA(8/7) 648 GAAGA MspA1I 170 CAGCTG C[AC]G^C[GT]G 533 CAGCTG MspI 88 CCGG C^CGG 620 CCGG MvaI 284 CCTGG CC^[AT]GG 675 CCAGG MwoI 84 GCTCCCGGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 172 GCTGCAGGGGC NdeII 423 GATC ^GATC 672 GATC NlaIV 558 GGTGCC GG[ACGT]^[ACGT]CC 741 GGTCCC NspV 215 TTCGAA TT^CGAA 264 TTCGAA PvuII 170 CAGCTG CAG^CTG 533 CAGCTG Sau3AI 423 GATC ^GATC 672 GATC SfaNI 48 GATGC GCATC(5/9) 519 GCATC SfcI 173 CTGCAG C^T[GA][CT]AG 636 CTACAG SfuI 215 TTCGAA TT^CGAA 264 TTCGAA SmlI 253 CTTGAG C^T[CT][GA]AG 505 CTCAAG StuI 551 AGGCCT AGG^CCT 689 AGGCCT TfiI 262 GATTC G^A[AT]TC 389 GAATC TseI 66 GCTGC G^C[AT]GC 172 GCTGC Tsp45I 236 GTGAC ^GT[GC]AC 499 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 21 enzymes) # ######################################### AluI AvaII BpmI BsmAI BssKI Cac8I Cfr13I DdeI Fnu4HI HaeIII HinfI ItaI MnlI MseI NlaIII Sau96I ScrFI TaqI Tru9I Tsp509I TthHB8I