Restriction map for
pFN21AB5966
(Circular) (Six-base) MAPSORT of: pFN21AB5966.seq Check: 5145 from: 1 to: 6370
pFN21AB5966 6370 bp
With 182 enzymes: SgfI *
August 20, 2012 11:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3110 3110
Size: 6370
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4180 278
Size: 53 83 186 936 2644 2468
Fragments arranged by size:
2644 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3459 3713 1937
Size: 1522 254 4594
Fragments arranged by size:
4594 1522 254
Acc65I G'GTAC_C
Cuts at: 3691 3691
Size: 6370
AclI AA'CG_TT
Cuts at: 1563 3669 4497 4870 1563
Size: 2106 828 373 3063
Fragments arranged by size:
3063 2106 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2916 2942 4433 5817 6085 1632
Size: 1284 26 1491 1384 268 1917
Fragments arranged by size:
1917 1491 1384 1284 268 26
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6173
AflIII A'CryG_T
Cuts at: 1203 5269 6246 1203
Size: 4066 977 1327
Fragments arranged by size:
4066 1327 977
AgeI A'CCGG_T
Cuts at: 5201 5201
Size: 6370
AhdI GACnn_n'nnGTC
Cuts at: 5099 5099
Size: 6370
AleI CACnn'nnGTG
Cuts at: 1166 2015 1166
Size: 849 5521
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6370
AlwNI CAG_nnn'CTG
Cuts at: 2243 2965 4113 5685 2243
Size: 722 1148 1572 2928
Fragments arranged by size:
2928 1572 1148 722
ApaI G_GGCC'C
Cuts at: 2237 2366 2237
Size: 129 6241
ApaLI G'TGCA_C
Cuts at: 4427 5583 4427
Size: 1156 5214
ApoI r'AATT_y
Cuts at: 1493 2684 3164 3679 3905 4057 6166 1493
Size: 1191 480 515 226 152 2109 1697
Fragments arranged by size:
2109 1697 1191 515 480 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4924 160
Size: 872 3892 1606
Fragments arranged by size:
3892 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6370
AvaI C'yCGr_G
Cuts at: 1870 1943 3695 1870
Size: 73 1752 4545
Fragments arranged by size:
4545 1752 73
BamHI G'GATC_C
Cuts at: 3700 4067 3700
Size: 367 6003
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2227 3691 5146 618
Size: 325 217 627 440 1464 1455 1842
Fragments arranged by size:
1842 1464 1455 627 440 325 217
BanII G_rGCy'C
Cuts at: 1391 2165 2237 2366 2751 3080 3489 3689 1391
Size: 774 72 129 385 329 409 200 4072
Fragments arranged by size:
4072 774 409 385 329 200 129 72
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2119 928
Size: 971 220 5179
Fragments arranged by size:
5179 971 220
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3064 3098 4561 4595 1903
Size: 34 50 34 1043 34 1463 34 3678
Fragments arranged by size:
3678 1463 1043 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4262 5472 4262
Size: 1210 5160
BclI T'GATC_A
Cuts at: 1548 3183 1548
Size: 1635 4735
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2376 3260 4981
Size: 107 122 71 631 605 704 884 1721
Cuts at: 4981 136
Size: 1525
Fragments arranged by size:
1721 1525 884 704 631 605 122 107
71
BglII A'GATC_T
Cuts at: 6365 6365
Size: 6370
BlpI GC'TnA_GC
Cuts at: 3369 3787 3369
Size: 418 5952
Bme1580I G_kGCm'C
Cuts at: 1790 2237 2366 4431 5587 1790
Size: 447 129 2065 1156 2573
Fragments arranged by size:
2573 2065 1156 447 129
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6370
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2369 4101 5059
Size: 416 53 83 399 24 952 1732 958
Cuts at: 5059 442
Size: 1753
Fragments arranged by size:
1753 1732 958 952 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2762 1056
Size: 1706 4664
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2051 2218 2595 2809 3546 5030 1239
Size: 812 167 377 214 737 1484 2579
Fragments arranged by size:
2579 1484 812 737 377 214 167
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3503 4495 5360 5658 3503
Size: 992 865 298 4215
Fragments arranged by size:
4215 992 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3569 5033 882
Size: 617 2070 1464 2219
Fragments arranged by size:
2219 2070 1464 617
BsaAI yAC'GTr
Cuts at: 493 1204 2116 2607 4161 493
Size: 711 912 491 1554 2702
Fragments arranged by size:
2702 1554 912 711 491
BsaBI GATnn'nnATC
Cuts at: 6364 6364
Size: 6370
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2393 3211 3402 4177
Size: 53 83 186 936 860 818 191 775
Cuts at: 4177 4559 5194 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 860 818 775 635 382 191
186 83 53
BsaWI w'CCGG_w
Cuts at: 4803 5201 5475 5622 6033 4803
Size: 398 274 147 411 5140
Fragments arranged by size:
5140 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3519 3549 1178
Size: 30 165 30 2116 30 3999
Fragments arranged by size:
3999 2116 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3018 1186
Size: 1832 4538
BseYI C'CCAG_C
Cuts at: 2379 2762 3169 5573 6069 2379
Size: 383 407 2404 496 2680
Fragments arranged by size:
2680 2404 496 407 383
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5797
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3202 3820 4581 5609 1540
Size: 275 186 1201 618 761 1028 2301
Fragments arranged by size:
2301 1201 1028 761 618 275 186
BsiHKAI G_wGCw'C
Cuts at: 2303 3080 3087 3489 4431 4516 5587 2303
Size: 777 7 402 942 85 1071 3086
Fragments arranged by size:
3086 1071 942 777 402 85 7
BsmI GAATG_Cn'
Cuts at: 2896 3881 3974 2896
Size: 985 93 5292
Fragments arranged by size:
5292 985 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2384 2384
Size: 6370
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2165 2237 2303 2366 2751 3080 3087
Size: 399 375 72 66 63 385 329 7
Cuts at: 3087 3489 3689 4431 4516 5587 1391
Size: 402 200 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 402 399 385 375 329
200 85 72 66 63 7
BspEI T'CCGG_A
Cuts at: 6033 6033
Size: 6370
BspHI T'CATG_A
Cuts at: 3180 4259 5989 3180
Size: 1079 1730 3561
Fragments arranged by size:
3561 1730 1079
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2251 3110 3725 844
Size: 1407 859 615 3489
Fragments arranged by size:
3489 1407 859 615
BsrBI CCG'CTC
Cuts at: 1394 1823 4257 1394
Size: 429 2434 3507
Fragments arranged by size:
3507 2434 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4865 5039 65
Size: 1183 3617 174 1396
Fragments arranged by size:
3617 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3462 5014 5201 1700
Size: 110 1652 1552 187 2869
Fragments arranged by size:
2869 1652 1552 187 110
BsrGI T'GTAC_A
Cuts at: 96 3060 96
Size: 2964 3406
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6370
BssSI C'ACGA_G
Cuts at: 3073 4430 5442 3073
Size: 1357 1012 4001
Fragments arranged by size:
4001 1357 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6370
BstBI TT'CG_AA
Cuts at: 5174 5174
Size: 6370
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6370
BstXI CCAn_nnnn'nTGG
Cuts at: 2017 2116 2017
Size: 99 6271
BstYI r'GATC_y
Cuts at: 1972 3700 4067 4452 4469 5921 6007 6019 6365
Size: 1728 367 385 17 1452 86 12 346
Cuts at: 6365 1972
Size: 1977
Fragments arranged by size:
1977 1728 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2016 513
Size: 552 951 4867
Fragments arranged by size:
4867 951 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2905 487
Size: 683 56 1679 3952
Fragments arranged by size:
3952 1679 683 56
BtsI GCAGTG_nn'
Cuts at: 3886 4680 4700 3886
Size: 794 20 5556
Fragments arranged by size:
5556 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6370
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6335
DraI TTT'AAA
Cuts at: 3674 3674
Size: 6370
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5377 809
Size: 507 4061 1802
Fragments arranged by size:
4061 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2172 2325 2527
Size: 54 1425 50 275 18 342 153 202
Cuts at: 2527 2586 3817 4706 6335 8
Size: 59 1231 889 1629 43
Fragments arranged by size:
1629 1425 1231 889 342 275 202 153
59 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3817 1537
Size: 275 2005 4090
Fragments arranged by size:
4090 2005 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2970 4300 1353
Size: 1617 1330 3423
Fragments arranged by size:
3423 1617 1330
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2710 4957 5331 5477 387
Size: 1064 138 1121 2247 374 146 1280
Fragments arranged by size:
2247 1280 1121 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3078 3487 3078
Size: 409 5961
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2051 2218 2595 2809 2916 2942 3546
Size: 393 419 167 377 214 107 26 604
Cuts at: 3546 4433 5030 5817 6085 1239
Size: 887 597 787 268 1524
Fragments arranged by size:
1524 887 787 604 597 419 393 377
268 214 167 107 26
EcoO109I rG'GnC_Cy
Cuts at: 2240 2363 3094 2240
Size: 123 731 5516
Fragments arranged by size:
5516 731 123
EcoRI G'AATT_C
Cuts at: 3164 3679 4057 3164
Size: 515 378 5477
Fragments arranged by size:
5477 515 378
EcoRV GAT'ATC
Cuts at: 2702 2702
Size: 6370
FspI TGC'GCA
Cuts at: 4876 4876
Size: 6370
HaeII r_GCGC'y
Cuts at: 1678 2278 2965 5517 1678
Size: 600 687 2552 2531
Fragments arranged by size:
2552 2531 687 600
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5011 5043 5085 5117 1373
Size: 32 135 32 3439 32 42 32 2626
Fragments arranged by size:
3439 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3714 3960 669
Size: 988 281 1776 246 3079
Fragments arranged by size:
3079 1776 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3564 748
Size: 2816 3554
HpaI GTT'AAC
Cuts at: 3960 3960
Size: 6370
KpnI G_GTAC'C
Cuts at: 3695 3695
Size: 6370
MfeI C'AATT_G
Cuts at: 3969 3969
Size: 6370
MluI A'CGCG_T
Cuts at: 6246 6246
Size: 6370
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2430 5484 5668 1305
Size: 1125 3054 184 2007
Fragments arranged by size:
3054 2007 1125 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2327 2588 10
Size: 54 1425 343 495 261 3792
Fragments arranged by size:
3792 1425 495 343 261 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2015 2114 3107 4328 4687 4846 518
Size: 648 849 99 993 1221 359 159 2042
Fragments arranged by size:
2042 1221 993 849 648 359 159 99
MspA1I CmG'CkG
Cuts at: 1606 2154 2573 3173 3493 3786 4463 5611 5856
Size: 548 419 600 320 293 677 1148 245
Cuts at: 5856 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 600 548 419 320 293
245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5818
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6370
NheI G'CTAG_C
Cuts at: 1052 2758 1052
Size: 1706 4664
NotI GC'GGCC_GC
Cuts at: 3817 3817
Size: 6370
NspI r_CATG'y
Cuts at: 1132 1229 3728 5273 1132
Size: 97 2499 1545 2229
Fragments arranged by size:
2499 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6370
PciI A'CATG_T
Cuts at: 5269 5269
Size: 6370
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2442 2638 1108
Size: 1334 196 4840
Fragments arranged by size:
4840 1334 196
PfoI T'CCnGG_A
Cuts at: 2028 2441 2028
Size: 413 5957
PmeI GTTT'AAAC
Cuts at: 3674 3674
Size: 6370
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4439 4471 5952 5984 4439
Size: 32 1481 32 4825
Fragments arranged by size:
4825 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2240 2240
Size: 6370
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6370
PsiI TTA'TAA
Cuts at: 3940 3940
Size: 6370
PspOMI G'GGCC_C
Cuts at: 2233 2362 2233
Size: 129 6241
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3053 3085 3053
Size: 32 6338
PstI C_TGCA'G
Cuts at: 830 2209 2572 3722 830
Size: 1379 363 1150 3478
Fragments arranged by size:
3478 1379 1150 363
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6370
PvuII CAG'CTG
Cuts at: 2573 3173 2573
Size: 600 5770
SacI G_AGCT'C
Cuts at: 3080 3489 3080
Size: 409 5961
SalI G'TCGA_C
Cuts at: 1936 3712 1936
Size: 1776 4594
SbfI CC_TGCA'GG
Cuts at: 3722 3722
Size: 6370
ScaI AGT'ACT
Cuts at: 4618 6330 4618
Size: 1712 4658
SexAI A'CCwGG_T
Cuts at: 2424 3277 2424
Size: 853 5517
SfcI C'TryA_G
Cuts at: 826 1045 2205 2550 2568 3718 4853 5534 5725
Size: 219 1160 345 18 1150 1135 681 191
Cuts at: 5725 826
Size: 1471
Fragments arranged by size:
1471 1160 1150 1135 681 345 219 191
18
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6370
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6370
SmaI CCC'GGG
Cuts at: 1872 3697 1872
Size: 1825 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3482 4474 5375 5637 820
Size: 197 926 1539 992 901 262 1553
Fragments arranged by size:
1553 1539 992 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4161 493
Size: 3668 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6370
SphI G_CATG'C
Cuts at: 1132 3728 1132
Size: 2596 3774
SspI AAT'ATT
Cuts at: 5 52 4294 5
Size: 47 4242 2081
Fragments arranged by size:
4242 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6370
StyI C'CwwG_G
Cuts at: 513 1065 2632 3221 3809 513
Size: 552 1567 589 588 3074
Fragments arranged by size:
3074 1567 589 588 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2371 4397 4414 4567 4752 5197 325
Size: 1255 791 2026 17 153 185 445 1498
Fragments arranged by size:
2026 1498 1255 791 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3060 4616 6328
Size: 274 80 33 51 1443 1083 1556 1712
Cuts at: 6328 96
Size: 138
Fragments arranged by size:
1712 1556 1443 1083 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3398 3430 3519 3551 6330 6362 3398
Size: 32 89 32 2779 32 3406
Fragments arranged by size:
3406 2779 89 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2024 3017 1316
Size: 708 993 4669
Fragments arranged by size:
4669 993 708
XbaI T'CTAG_A
Cuts at: 3706 3706
Size: 6370
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2604 2604
Size: 6370
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6370
XmaI C'CCGG_G
Cuts at: 1870 3695 1870
Size: 1825 4545
XmnI GAAnn'nnTTC
Cuts at: 4499 4499
Size: 6370
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4178 276
Size: 53 83 186 936 2644 2468
Fragments arranged by size:
2644 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoNI FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspXI RsrII SacII SanDI SapI SfiI
SfoI SrfI SwaI