Restriction map for
pFN21AB5966
(Linear) (Six-base) MAPSORT of: KIBB5966.seq Check: 2113 from: 1 to: 1665
KIBB5966.orf
With 182 enzymes: SgfI *
January 6, 2012 11:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1105 1665
Size: 1105 560
AccI GT'mk_AC
Cuts at: 0 1454 1665
Size: 1454 211
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 911 937 1665
Size: 911 26 728
Fragments arranged by size:
911 728 26
AleI CACnn'nnGTG
Cuts at: 0 10 1665
Size: 10 1655
AlwNI CAG_nnn'CTG
Cuts at: 0 238 960 1665
Size: 238 722 705
Fragments arranged by size:
722 705 238
ApaI G_GGCC'C
Cuts at: 0 232 361 1665
Size: 232 129 1304
Fragments arranged by size:
1304 232 129
ApoI r'AATT_y
Cuts at: 0 679 1159 1665
Size: 679 480 506
Fragments arranged by size:
679 506 480
BanI G'GyrC_C
Cuts at: 0 222 1665
Size: 222 1443
BanII G_rGCy'C
Cuts at: 0 160 232 361 746 1075 1484 1665
Size: 160 72 129 385 329 409 181
Fragments arranged by size:
409 385 329 181 160 129 72
BbsI GAAGACnn'nnnn_
Cuts at: 0 114 1665
Size: 114 1551
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1059 1093 1665
Size: 1059 34 572
Fragments arranged by size:
1059 572 34
BclI T'GATC_A
Cuts at: 0 1178 1665
Size: 1178 487
BglI GCCn_nnn'nGGC
Cuts at: 0 371 1255 1665
Size: 371 884 410
Fragments arranged by size:
884 410 371
BlpI GC'TnA_GC
Cuts at: 0 1364 1665
Size: 1364 301
Bme1580I G_kGCm'C
Cuts at: 0 232 361 1665
Size: 232 129 1304
Fragments arranged by size:
1304 232 129
BmrI ACTGGGnnnn_n'
Cuts at: 0 364 1665
Size: 364 1301
BmtI G_CTAG'C
Cuts at: 0 757 1665
Size: 757 908
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 213 590 804 1541 1665
Size: 46 167 377 214 737 124
Fragments arranged by size:
737 377 214 167 124 46
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1498 1665
Size: 1498 167
BsaI GGTCTCn'nnnn_
Cuts at: 0 1564 1665
Size: 1564 101
BsaAI yAC'GTr
Cuts at: 0 111 602 1665
Size: 111 491 1063
Fragments arranged by size:
1063 491 111
BsaHI Gr'CG_yC
Cuts at: 0 388 1206 1397 1665
Size: 388 818 191 268
Fragments arranged by size:
818 388 268 191
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1514 1544 1665
Size: 1514 30 121
Fragments arranged by size:
1514 121 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1013 1665
Size: 1013 652
BseYI C'CCAG_C
Cuts at: 0 374 757 1164 1665
Size: 374 383 407 501
Fragments arranged by size:
501 407 383 374
BsiEI CG_ry'CG
Cuts at: 0 1197 1665
Size: 1197 468
BsiHKAI G_wGCw'C
Cuts at: 0 298 1075 1082 1484 1665
Size: 298 777 7 402 181
Fragments arranged by size:
777 402 298 181 7
BsmI GAATG_Cn'
Cuts at: 0 891 1665
Size: 891 774
BsmBI CGTCTCn'nnnn_
Cuts at: 0 379 1665
Size: 379 1286
Bsp1286I G_dGCh'C
Cuts at: 0 160 232 298 361 746 1075 1082 1484
Size: 160 72 66 63 385 329 7 402
Cuts at: 1484 1665
Size: 181
Fragments arranged by size:
402 385 329 181 160 72 66 63
7
BspHI T'CATG_A
Cuts at: 0 1175 1665
Size: 1175 490
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 246 1105 1665
Size: 246 859 560
Fragments arranged by size:
859 560 246
BsrFI r'CCGG_y
Cuts at: 0 1457 1665
Size: 1457 208
BsrGI T'GTAC_A
Cuts at: 0 1055 1665
Size: 1055 610
BssSI C'ACGA_G
Cuts at: 0 1068 1665
Size: 1068 597
BstXI CCAn_nnnn'nTGG
Cuts at: 0 12 111 1665
Size: 12 99 1554
Fragments arranged by size:
1554 99 12
BtgI C'CryG_G
Cuts at: 0 11 1665
Size: 11 1654
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 900 1665
Size: 900 765
EaeI y'GGCC_r
Cuts at: 0 167 320 522 581 1665
Size: 167 153 202 59 1084
Fragments arranged by size:
1084 202 167 153 59
EarI CTCTTCn'nnn_
Cuts at: 0 965 1665
Size: 965 700
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 705 1665
Size: 705 960
EcoICRI GAG'CTC
Cuts at: 0 1073 1482 1665
Size: 1073 409 183
Fragments arranged by size:
1073 409 183
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 213 590 804 911 937 1541 1665
Size: 46 167 377 214 107 26 604 124
Fragments arranged by size:
604 377 214 167 124 107 46 26
EcoO109I rG'GnC_Cy
Cuts at: 0 235 358 1089 1665
Size: 235 123 731 576
Fragments arranged by size:
731 576 235 123
EcoRI G'AATT_C
Cuts at: 0 1159 1665
Size: 1159 506
EcoRV GAT'ATC
Cuts at: 0 697 1665
Size: 697 968
HaeII r_GCGC'y
Cuts at: 0 273 960 1665
Size: 273 687 705
Fragments arranged by size:
705 687 273
HindIII A'AGCT_T
Cuts at: 0 1559 1665
Size: 1559 106
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 425 1665
Size: 425 1240
MscI TGG'CCA
Cuts at: 0 322 583 1665
Size: 322 261 1082
Fragments arranged by size:
1082 322 261
MslI CAynn'nnrTG
Cuts at: 0 10 109 1102 1665
Size: 10 99 993 563
Fragments arranged by size:
993 563 99 10
MspA1I CmG'CkG
Cuts at: 0 149 568 1168 1488 1665
Size: 149 419 600 320 177
Fragments arranged by size:
600 419 320 177 149
NheI G'CTAG_C
Cuts at: 0 753 1665
Size: 753 912
PflMI CCAn_nnn'nTGG
Cuts at: 0 437 633 1665
Size: 437 196 1032
Fragments arranged by size:
1032 437 196
PfoI T'CCnGG_A
Cuts at: 0 23 436 1665
Size: 23 413 1229
Fragments arranged by size:
1229 413 23
PpuMI rG'GwC_Cy
Cuts at: 0 235 1665
Size: 235 1430
PspOMI G'GGCC_C
Cuts at: 0 228 357 1665
Size: 228 129 1308
Fragments arranged by size:
1308 228 129
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1048 1080 1665
Size: 1048 32 585
Fragments arranged by size:
1048 585 32
PstI C_TGCA'G
Cuts at: 0 204 567 1665
Size: 204 363 1098
Fragments arranged by size:
1098 363 204
PvuII CAG'CTG
Cuts at: 0 568 1168 1665
Size: 568 600 497
Fragments arranged by size:
600 568 497
SacI G_AGCT'C
Cuts at: 0 1075 1484 1665
Size: 1075 409 181
Fragments arranged by size:
1075 409 181
SexAI A'CCwGG_T
Cuts at: 0 419 1272 1665
Size: 419 853 393
Fragments arranged by size:
853 419 393
SfcI C'TryA_G
Cuts at: 0 200 545 563 1665
Size: 200 345 18 1102
Fragments arranged by size:
1102 345 200 18
SmlI C'TyrA_G
Cuts at: 0 1477 1665
Size: 1477 188
StyI C'CwwG_G
Cuts at: 0 627 1216 1665
Size: 627 589 449
Fragments arranged by size:
627 589 449
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 366 1665
Size: 366 1299
TatI w'GTAC_w
Cuts at: 0 1055 1665
Size: 1055 610
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1393 1425 1514 1546 1665
Size: 1393 32 89 32 119
Fragments arranged by size:
1393 119 89 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 19 1012 1665
Size: 19 993 653
Fragments arranged by size:
993 653 19
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 599 1665
Size: 599 1066
Enzymes that do cut:
AarI AccI AcuI AleI AlwNI ApaI ApoI BanI
BanII BbsI BcgI BclI BglI BlpI Bme1580I BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaHI BsaXI BseRI
BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrFI BsrGI BssSI BstXI BtgI BtgZI EaeI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV HaeII HindIII
MmeI MscI MslI MspA1I NheI PflMI PfoI PpuMI
PspOMI PsrI PstI PvuII SacI SexAI SfcI SmlI
StyI TaqII TatI TstI Tth111I XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbeI BbvCI BciVI BfrBI BglII BmgBI
BplI Bpu10I BsaBI BsaWI BsgI BsiWI BspEI BsrBI
BsrDI BssHII BstAPI BstBI BstEII BstYI BstZ17I Bsu36I
BtsI ClaI CspCI DraI DraIII DrdI EagI EcoNI
FalI FseI FspI FspAI Hin4I HincII HpaI KasI
KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV
NotI NruI NsiI NspI PacI PasI PciI PmeI
PmlI PpiI PshAI PsiI PspXI PvuI RsrII SacII
SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI XbaI XhoI XmaI XmnI ZraI