Restriction map for
pFN21AE2716
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# NO CUT ENZYMES ( 176 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
Alw44I AlwI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspI AvaI AviII AvrII
BamHI BanI BanII BbeI BbrPI
BbvI BcgI BclI BcnI BfrI
BlnI BlpI BpmI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsmBI
BsmFI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I Cac8I CelII CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI Fnu4HI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HpaI HpaII
ItaI KasI KpnI KspI MamI
MfeI MluI MroI MseI MslI
MspI MunI MvnI NaeI NarI
NciI NdeI NgoMIV NheI NlaIV
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfaNI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tru9I TseI Tth111I
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AcsI 459 AAATTC [GA]^AATT[CT]
AhdI 616 GACTACTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 384 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 459 AAATTC [GA]^AATT[CT]
AspEI 616 GACTACTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 299 GTGCTC G[AT]GC[AT]^C
BbvCI 408 CCTCAGC CCTCAGC(-5/-2)
BciVI 220 GTATCC GTATCC(6/5)
BfaI 335 CTAG C^TAG
BglI 380 GCCACAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 331 AGATCT A^GATCT
BmyI 299 GTGCTC G[AGT]GC[ACT]^C
BsiHKAI 299 GTGCTC G[AT]GC[AT]^C
BsiYI 408 CCTCAGCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 408 CCTCAGCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI 691 GCATTC GAATGC(1/-1)
Bsp1286I 299 GTGCTC G[AGT]GC[ACT]^C
BspMI 666 ACCTGC ACCTGC(4/8)
BsrDI 513 GCAATG GCAATG(2/0)
BstF5I 502 CATCC GGATG(2/0)
BstYI 331 AGATCT [GA]^GATC[CT]
DpnII 332 GATC ^GATC
DraII 197 AGGACCT [GA]G^G[ACGT]CC[CT]
Eam1105I 616 GACTACTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 290 GAAGAG CTCTTC(1/4)
EcoO109I 197 AGGACCT [GA]G^G[ACGT]CC[CT]
FokI 502 CATCC GGATG(9/13)
HindIII 440 AAGCTT A^AGCTT
Ksp632I 290 GAAGAG CTCTTC(1/4)
MaeI 335 CTAG C^TAG
MaeII 396 ACGT A^CGT
MboI 332 GATC ^GATC
MflI 331 AGATCT [GA]^GATC[CT]
MspA1I 547 CAGCTG C[AC]G^C[GT]G
NdeII 332 GATC ^GATC
PpuMI 197 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 547 CAGCTG CAG^CTG
SapI 290 GAAGAGC GCTCTTC(1/4)
Sau3AI 332 GATC ^GATC
Tsp45I 553 GTGAC ^GT[GC]AC
XcmI 181 CCAGAAAACTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 331 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 24 enzymes) #
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AciI 378 CCGC CCGC(-3/-1)
658 CCGC
AfaI 52 GTAC GT^AC
241 GTAC
AvaII 198 GGACC G^G[AT]CC
623 GGTCC
BalI 178 TGGCCA TGG^CCA
416 TGGCCA
BbsI 339 GTCTTC GAAGAC(2/6)
635 GTCTTC
BpuAI 339 GTCTTC GAAGAC(2/6)
635 GTCTTC
BsmAI 279 GTCTC GTCTC(1/5)
422 GTCTC
BssKI 414 CCTGG ^CC[ACGT]GG
447 CCTGG
BstNI 414 CCTGG CC^[AT]GG
447 CCTGG
EaeI 178 TGGCCA [CT]^GGCC[GA]
416 TGGCCA
EcoRII 414 CCTGG ^CC[AT]GG
447 CCTGG
EcoT14I 175 CCATGG C^C[AT][AT]GG
224 CCATGG
HphI 552 GGTGA GGTGA(8/7)
425 TCACC
MaeIII 553 GTGAC ^GT[ACGT]AC
629 GTAAC
MluNI 178 TGGCCA TGG^CCA
416 TGGCCA
MscI 178 TGGCCA TGG^CCA
416 TGGCCA
MvaI 414 CCTGG CC^[AT]GG
447 CCTGG
MwoI 380 GCCACAGTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
404 GCAACCTCAGC
NcoI 175 CCATGG C^CATGG
224 CCATGG
RsaI 52 GTAC GT^AC
241 GTAC
ScrFI 414 CCTGG CC^[ACGT]GG
447 CCTGG
StyI 175 CCATGG C^C[AT][AT]GG
224 CCATGG
TfiI 68 GAATC G^A[AT]TC
700 GATTC
Tsp509I 34 AATT ^AATT
460 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 16 enzymes) #
#########################################
AluI BsaJI BsrI Cfr13I DdeI
DsaI HaeIII HinfI MboII MnlI
NlaIII PleI Sau96I TaqI TspRI
TthHB8I