################################## # NO CUT ENZYMES ( 155 enzymes) # ################################## AatII AccI AccII AccIII AclI AcyI AflII AflIII AgeI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I AspI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BclI BcnI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraIII DrdI EaeI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII HapII HgaI Hin1I HincII HindII HindIII HpaI HpaII KasI KspI MamI MfeI MluI MluNI MroI MscI MslI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NotI NruI NsiI NspI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### Acc65I 797 GGTACC G^GTACC AcsI 274 GAATTT [GA]^AATT[CT] AhdI 299 GACGGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 53 GGATC GGATC(4/5) ApoI 274 GAATTT [GA]^AATT[CT] Asp718I 797 GGTACC G^GTACC AspEI 299 GACGGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 745 GTGCTC G[AT]GC[AT]^C AvaI 378 CCCGAG C^[CT]CG[GA]G BbsI 136 GAAGAC GAAGAC(2/6) BcgI 438 GCACAAGATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 555 GGATAC GTATCC(6/5) BpuAI 136 GAAGAC GAAGAC(2/6) BsaI 266 GGTCTC GGTCTC(1/5) BsgI 160 CTGCAC GTGCAG(16/14) BsiHKAI 745 GTGCTC G[AT]GC[AT]^C BsmBI 297 GAGACG CGTCTC(1/5) BsmI 687 GAATGC GAATGC(1/-1) BsoBI 378 CCCGAG C^[CT]CG[GA]G BsrI 536 ACTGG ACTGG(1/-1) BstBI 446 TTCGAA TT^CGAA BstYI 53 GGATCT [GA]^GATC[CT] CfoI 834 GCGC GCG^C DraI 58 TTTAAA TTT^AAA DraII 422 AGGACCC [GA]G^G[ACGT]CC[CT] Eam1105I 299 GACGGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 466 GAAGAG CTCTTC(1/4) EcoO109I 422 AGGACCC [GA]G^G[ACGT]CC[CT] FauI 760 CCCGC CCCGC(4/6) HhaI 834 GCGC GCG^C HinP1I 834 GCGC G^CGC KpnI 797 GGTACC GGTAC^C Ksp632I 466 GAAGAG CTCTTC(1/4) MaeII 431 ACGT A^CGT MflI 53 GGATCT [GA]^GATC[CT] NheI 672 GCTAGC G^CTAGC NspV 446 TTCGAA TT^CGAA PflMI 849 CCAGATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 12 GACTC GAGTC(4/5) PpuMI 422 AGGACCC [GA]G^G[AT]CC[CT] PstI 682 CTGCAG CTGCA^G ScaI 548 AGTACT AGT^ACT SfcI 682 CTGCAG C^T[GA][CT]AG SfuI 446 TTCGAA TT^CGAA Tsp509I 275 AATT ^AATT Van91I 849 CCAGATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 53 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### BanI 797 GGTACC G^G[CT][GA]CC 819 GGTGCC BanII 386 GGGCTC G[GA]GC[CT]^C 397 GAGCCC BfaI 101 CTAG C^TAG 673 CTAG BsmAI 297 GAGAC GTCTC(1/5) 267 GTCTC BsmFI 495 GGGAC GGGAC(10/14) 331 GTCCC BspMI 158 ACCTGC ACCTGC(4/8) 691 GCAGGT Cac8I 118 GCTCGC GC[ACGT]^[ACGT]GC 672 GCTAGC DsaI 791 CCGTGG C^C[GA][CT]GG 815 CCACGG HphI 699 GGTGA GGTGA(8/7) 858 GGTGA MaeI 101 CTAG C^TAG 673 CTAG SmlI 517 CTCAAG C^T[CT][GA]AG 829 CTCAAG TaqI 447 TCGA T^CGA 735 TCGA TthHB8I 447 TCGA T^CGA 735 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 43 enzymes) # ######################################### AciI AfaI AluI AvaII BbvI BmyI BsaJI BseRI BsiYI BslI Bsp1286I BssKI BstF5I BstNI Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HinfI ItaI MaeIII MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI TfiI Tru9I TseI Tsp45I TspRI