################################## # NO CUT ENZYMES ( 156 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BclI BfrI BglI BglII BlnI BmyI BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstXI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraII DrdI Eam1105I Eco47III Eco81I EcoNI EcoO109I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MaeII MamI MfeI MluI MluNI MroI MscI MseI MslI MunI NaeI NarI NdeI NgoMIV NheI NsiI NspI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tru9I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AcsI 180 GAATTT [GA]^AATT[CT] AfaI 51 GTAC GT^AC AlwNI 684 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 180 GAATTT [GA]^AATT[CT] AspI 208 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC BciVI 400 GGATAC GTATCC(6/5) BcnI 71 CCGGG CC^[GC]GG BfaI 232 CTAG C^TAG BlpI 650 GCTTAGC GC^T[ACGT]AGC Bpu1102I 650 GCTTAGC GC^T[ACGT]AGC BsaI 555 GGTCTC GGTCTC(1/5) BseRI 448 CTCCTC GAGGAG(10/8) BsiEI 8 CGGCCG CG[GA][CT]^CG BstEII 226 GGTCACC G^GT[ACGT]ACC BstPI 226 GGTCACC G^GT[ACGT]ACC BstYI 412 GGATCT [GA]^GATC[CT] CelII 650 GCTTAGC GC^T[ACGT]AGC CfoI 33 GCGC GCG^C DraIII 344 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 8 CGGCCG [CT]^GGCC[GA] EagI 8 CGGCCG C^GGCCG EclXI 8 CGGCCG C^GGCCG Eco52I 8 CGGCCG C^GGCCG EcoO65I 226 GGTCACC G^GT[ACGT]ACC EcoT14I 638 CCATGG C^C[AT][AT]GG HapII 71 CCGG C^CGG HgaI 88 GACGC GACGC(5/10) HhaI 33 GCGC GCG^C HinP1I 33 GCGC G^CGC HpaII 71 CCGG C^CGG MaeI 232 CTAG C^TAG MflI 412 GGATCT [GA]^GATC[CT] MspI 71 CCGG C^CGG NciI 71 CCGGG CC^[GC]GG NcoI 638 CCATGG C^CATGG NotI 7 GCGGCCGC GC^GGCCGC NruI 38 TCGCGA TCG^CGA PflFI 208 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 457 GACTC GAGTC(4/5) PstI 364 CTGCAG CTGCA^G PvuII 241 CAGCTG CAG^CTG RsaI 51 GTAC GT^AC SapI 235 GAAGAGC GCTCTTC(1/4) SfcI 364 CTGCAG C^T[GA][CT]AG StyI 638 CCATGG C^C[AT][AT]GG TfiI 42 GATTC G^A[AT]TC Tsp509I 181 AATT ^AATT Tth111I 208 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoII 412 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### BsmAI 566 GAGAC GTCTC(1/5) 556 GTCTC BspMI 53 ACCTGC ACCTGC(4/8) 366 GCAGGT DsaI 408 CCGTGG C^C[GA][CT]GG 638 CCATGG EarI 235 GAAGAG CTCTTC(1/4) 609 GAAGAG FauI 19 CCCGC CCCGC(4/6) 81 GCGGG HinfI 42 GATTC G^A[ACGT]TC 457 GACTC HphI 113 TCACC GGTGA(8/7) 228 TCACC Ksp632I 235 GAAGAG CTCTTC(1/4) 609 GAAGAG MaeIII 206 GTGAC ^GT[ACGT]AC 227 GTCAC MspA1I 5 CAGCGG C[AC]G^C[GT]G 241 CAGCTG NlaIII 136 CATG CATG^ 639 CATG TaqI 62 TCGA T^CGA 431 TCGA Tsp45I 206 GTGAC ^GT[GC]AC 227 GTCAC TthHB8I 62 TCGA T^CGA 431 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 39 enzymes) # ######################################### AccII AciI AluI AlwI AvaII BbvI BpmI BsaJI BsiYI BslI BsmFI BsrI BssKI BstF5I BstNI BstUI Cac8I Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII ItaI MboI MboII MnlI MvaI MvnI MwoI NdeII NlaIV Sau3AI Sau96I ScrFI SfaNI TseI TspRI