(Circular) (Six-base) MAPSORT of: pFN21AE1741.seq Check: 2357 from: 1 to: 5437 pFN21AE1741 5437 bp With 182 enzymes: SgfI * August 16, 2010 14:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3247 278 Size: 53 83 186 936 1711 2468 Fragments arranged by size: 2468 1711 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2780 1937 Size: 843 4594 Acc65I G'GTAC_C Cuts at: 2758 2758 Size: 5437 AclI AA'CG_TT Cuts at: 1563 3564 3937 1563 Size: 2001 373 3063 Fragments arranged by size: 3063 2001 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3500 4884 5152 1632 Size: 1868 1384 268 1917 Fragments arranged by size: 1917 1868 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5240 AflIII A'CryG_T Cuts at: 1203 4336 5313 1203 Size: 3133 977 1327 Fragments arranged by size: 3133 1327 977 AgeI A'CCGG_T Cuts at: 4268 4268 Size: 5437 AhdI GACnn_n'nnGTC Cuts at: 4166 4166 Size: 5437 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5437 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2268 1184 Size: 1084 4353 AlwNI CAG_nnn'CTG Cuts at: 2694 3180 4752 2694 Size: 486 1572 3379 Fragments arranged by size: 3379 1572 486 ApaLI G'TGCA_C Cuts at: 3494 4650 3494 Size: 1156 4281 ApoI r'AATT_y Cuts at: 1493 2185 2746 2972 3124 5233 1493 Size: 692 561 226 152 2109 1697 Fragments arranged by size: 2109 1697 692 561 226 152 AseI AT'TA_AT Cuts at: 160 1032 3991 160 Size: 872 2959 1606 Fragments arranged by size: 2959 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5437 AvaI C'yCGr_G Cuts at: 1870 1943 2762 1870 Size: 73 819 4545 Fragments arranged by size: 4545 819 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2398 2431 2398 Size: 33 5404 BamHI G'GATC_C Cuts at: 2767 3134 2767 Size: 367 5070 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2758 4213 618 Size: 325 217 627 971 1455 1842 Fragments arranged by size: 1842 1455 971 627 325 217 BanII G_rGCy'C Cuts at: 1391 2756 1391 Size: 1365 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4466 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3628 3662 1903 Size: 34 1691 34 3678 Fragments arranged by size: 3678 1691 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2399 3329 4539 2399 Size: 930 1210 3297 Fragments arranged by size: 3297 1210 930 BclI T'GATC_A Cuts at: 1548 1548 Size: 5437 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4048 136 Size: 107 122 71 631 605 2376 1525 Fragments arranged by size: 2376 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5432 5432 Size: 5437 BlpI GC'TnA_GC Cuts at: 2656 2854 2656 Size: 198 5239 Bme1580I G_kGCm'C Cuts at: 1790 3498 4654 1790 Size: 1708 1156 2573 Fragments arranged by size: 2573 1708 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5437 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2346 3168 4126 Size: 416 53 83 399 24 929 822 958 Cuts at: 4126 442 Size: 1753 Fragments arranged by size: 1753 958 929 822 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5437 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2357 2517 2716 4097 1239 Size: 1118 160 199 1381 2579 Fragments arranged by size: 2579 1381 1118 199 160 Bpu10I CC'TnA_GC Cuts at: 2588 2588 Size: 5437 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3562 4427 4725 3562 Size: 865 298 4274 Fragments arranged by size: 4274 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2566 4100 882 Size: 617 1067 1534 2219 Fragments arranged by size: 2219 1534 1067 617 BsaAI yAC'GTr Cuts at: 493 1204 3228 493 Size: 711 2024 2702 Fragments arranged by size: 2702 2024 711 BsaBI GATnn'nnATC Cuts at: 5431 5431 Size: 5437 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3244 3626 4261 275 Size: 53 83 186 936 1711 382 635 1451 Fragments arranged by size: 1711 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3870 4268 4542 4689 5100 3870 Size: 398 274 147 411 4207 Fragments arranged by size: 4207 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5212 Fragments arranged by size: 5212 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2444 1186 Size: 1258 4179 BseYI C'CCAG_C Cuts at: 4640 5136 4640 Size: 496 4941 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4864 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2016 2887 3648 4676 1540 Size: 275 186 15 871 761 1028 2301 Fragments arranged by size: 2301 1028 871 761 275 186 15 BsiHKAI G_wGCw'C Cuts at: 3498 3583 4654 3498 Size: 85 1071 4281 Fragments arranged by size: 4281 1071 85 BsmI GAATG_Cn' Cuts at: 2948 3041 2948 Size: 93 5344 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2756 3498 3583 4654 1391 Size: 399 966 742 85 1071 2174 Fragments arranged by size: 2174 1071 966 742 399 85 BspEI T'CCGG_A Cuts at: 5100 5100 Size: 5437 BspHI T'CATG_A Cuts at: 3326 5056 3326 Size: 1730 3707 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2067 2362 2792 844 Size: 1223 295 430 3489 Fragments arranged by size: 3489 1223 430 295 BsrBI CCG'CTC Cuts at: 1394 1823 3324 1394 Size: 429 1501 3507 Fragments arranged by size: 3507 1501 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3932 4106 65 Size: 1183 2684 174 1396 Fragments arranged by size: 2684 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4081 4268 1700 Size: 110 2271 187 2869 Fragments arranged by size: 2869 2271 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5437 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5437 BssSI C'ACGA_G Cuts at: 3497 4509 3497 Size: 1012 4425 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5437 BstBI TT'CG_AA Cuts at: 4241 4241 Size: 5437 BstEII G'GTnAC_C Cuts at: 1184 2231 1184 Size: 1047 4390 BstYI r'GATC_y Cuts at: 1972 2417 2767 3134 3519 3536 4988 5074 5086 Size: 445 350 367 385 17 1452 86 12 Cuts at: 5086 5432 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 445 385 367 350 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2413 2643 513 Size: 552 939 409 230 3307 Fragments arranged by size: 3307 939 552 409 230 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4698 Fragments arranged by size: 4698 683 56 BtsI GCAGTG_nn' Cuts at: 2215 2953 3747 3767 2215 Size: 738 794 20 3885 Fragments arranged by size: 3885 794 738 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5437 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5402 DraI TTT'AAA Cuts at: 2741 2741 Size: 5437 DraIII CAC_nnn'GTG Cuts at: 2354 2354 Size: 5437 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4444 809 Size: 507 3128 1802 Fragments arranged by size: 3128 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2013 2884 3773 Size: 54 1425 50 275 18 183 871 889 Cuts at: 3773 5402 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 871 275 183 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2013 2884 1537 Size: 275 201 871 4090 Fragments arranged by size: 4090 871 275 201 EarI CTCTTCn'nnn_ Cuts at: 1353 2235 2609 3367 1353 Size: 882 374 758 3423 Fragments arranged by size: 3423 882 758 374 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2521 4024 4398 4544 387 Size: 1064 138 932 1503 374 146 1280 Fragments arranged by size: 1503 1280 1064 932 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2357 2517 2716 3500 4097 4884 5152 Size: 393 725 160 199 784 597 787 268 Cuts at: 5152 1239 Size: 1524 Fragments arranged by size: 1524 787 784 725 597 393 268 199 160 EcoRI G'AATT_C Cuts at: 2746 3124 2746 Size: 378 5059 FspI TGC'GCA Cuts at: 3943 3943 Size: 5437 HaeII r_GCGC'y Cuts at: 1678 4584 1678 Size: 2906 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2084 2116 4078 4110 4152 Size: 32 135 32 512 32 1962 32 42 Cuts at: 4152 4184 1373 Size: 32 2626 Fragments arranged by size: 2626 1962 512 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2781 3027 669 Size: 988 281 843 246 3079 Fragments arranged by size: 3079 988 843 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5437 HpaI GTT'AAC Cuts at: 3027 3027 Size: 5437 KpnI G_GTAC'C Cuts at: 2762 2762 Size: 5437 MfeI C'AATT_G Cuts at: 3036 3036 Size: 5437 MluI A'CGCG_T Cuts at: 5313 5313 Size: 5437 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4551 4735 1305 Size: 3246 184 2007 Fragments arranged by size: 3246 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3615 Fragments arranged by size: 3615 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3395 3754 3913 518 Size: 648 2229 359 159 2042 Fragments arranged by size: 2229 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2012 2248 2853 3530 4678 4923 1606 Size: 406 236 605 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 605 406 245 236 NcoI C'CATG_G Cuts at: 513 1065 2004 2643 513 Size: 552 939 639 3307 Fragments arranged by size: 3307 939 639 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5437 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5437 NotI GC'GGCC_GC Cuts at: 2013 2884 2013 Size: 871 4566 NruI TCG'CGA Cuts at: 2045 2045 Size: 5437 NspI r_CATG'y Cuts at: 1132 1229 2795 4340 1132 Size: 97 1566 1545 2229 Fragments arranged by size: 2229 1566 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5437 PciI A'CATG_T Cuts at: 4336 4336 Size: 5437 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5437 PfoI T'CCnGG_A Cuts at: 2075 2075 Size: 5437 PmeI GTTT'AAAC Cuts at: 2741 2741 Size: 5437 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3506 3538 5019 5051 3506 Size: 32 1481 32 3892 Fragments arranged by size: 3892 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5437 PsiI TTA'TAA Cuts at: 3007 3007 Size: 5437 PstI C_TGCA'G Cuts at: 830 2373 2789 830 Size: 1543 416 3478 Fragments arranged by size: 3478 1543 416 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5437 PvuII CAG'CTG Cuts at: 2248 2248 Size: 5437 SalI G'TCGA_C Cuts at: 1936 2779 1936 Size: 843 4594 SapI GCTCTTCn'nnn_ Cuts at: 2235 2235 Size: 5437 SbfI CC_TGCA'GG Cuts at: 2789 2789 Size: 5437 ScaI AGT'ACT Cuts at: 3685 5397 3685 Size: 1712 3725 SfcI C'TryA_G Cuts at: 826 1045 2369 2785 3920 4601 4792 826 Size: 219 1324 416 1135 681 191 1471 Fragments arranged by size: 1471 1324 1135 681 416 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5437 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5437 SmaI CCC'GGG Cuts at: 1872 2764 1872 Size: 892 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3541 4442 4704 820 Size: 197 926 1598 901 262 1553 Fragments arranged by size: 1598 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3228 493 Size: 2735 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5437 SphI G_CATG'C Cuts at: 1132 2795 1132 Size: 1663 3774 SspI AAT'ATT Cuts at: 5 52 3361 5 Size: 47 3309 2081 Fragments arranged by size: 3309 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5437 StyI C'CwwG_G Cuts at: 513 1065 2004 2643 2876 513 Size: 552 939 639 233 3074 Fragments arranged by size: 3074 939 639 552 233 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2365 2653 3464 3481 3634 3819 4264 Size: 1255 785 288 811 17 153 185 445 Cuts at: 4264 325 Size: 1498 Fragments arranged by size: 1498 1255 811 785 445 288 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3683 5395 96 Size: 274 80 33 51 1443 1706 1712 138 Fragments arranged by size: 1712 1706 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2371 2403 2674 2706 5397 5429 2371 Size: 32 271 32 2691 32 2379 Fragments arranged by size: 2691 2379 271 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2216 1316 Size: 900 4537 XbaI T'CTAG_A Cuts at: 2773 2773 Size: 5437 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5437 XmaI C'CCGG_G Cuts at: 1870 2762 1870 Size: 892 4545 XmnI GAAnn'nnTTC Cuts at: 3566 3566 Size: 5437 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3245 276 Size: 53 83 186 936 1711 2468 Fragments arranged by size: 2468 1711 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI