Restriction map for
pFN21AE1777
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# NO CUT ENZYMES ( 149 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BanII BbrPI
BbvCI BciVI BfrI BglI BglII
BlnI BpmI BsaBI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrFI BsrGI
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT14I FseI
FspI HincII HindII HindIII HpaI
KpnI Ksp632I KspI MamI MfeI
MluI MluNI MroI MscI MunI
NaeI NcoI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI ScaI SexAI SfiI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SspBI SspI StuI StyI SwaI
TaqI Tth111I TthHB8I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AcyI 193 GGCGCC G[GA]^CG[CT]C
AlwNI 656 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 353 GTGCTC G[AT]GC[AT]^C
BbeI 193 GGCGCC GGCGC^C
BbsI 567 GTCTTC GAAGAC(2/6)
BcgI 122 GCACCGTCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 167 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 167 GCTTAGC GC^T[ACGT]AGC
BpuAI 567 GTCTTC GAAGAC(2/6)
BsaHI 193 GGCGCC G[GA]^CG[CT]C
BsaI 96 GGTCTC GGTCTC(1/5)
BsaJI 671 CCCAGG C^C[ACGT][ACGT]GG
BsiHKAI 353 GTGCTC G[AT]GC[AT]^C
BsmAI 97 GTCTC GTCTC(1/5)
BsmFI 62 GGGAC GGGAC(10/14)
BspMI 152 ACCTGC ACCTGC(4/8)
BsrI 481 ACTGG ACTGG(1/-1)
BssHII 10 GCGCGC G^CGCGC
BstAPI 454 GCATGTTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 638 CCATTCATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 430 GGATCT [GA]^GATC[CT]
CelII 167 GCTTAGC GC^T[ACGT]AGC
DdeI 168 CTTAG C^T[ACGT]AG
HaeIII 177 GGCC GG^CC
HgaI 64 GACGC GACGC(5/10)
Hin1I 193 GGCGCC G[GA]^CG[CT]C
KasI 193 GGCGCC G^GCGCC
MflI 430 GGATCT [GA]^GATC[CT]
NarI 193 GGCGCC GG^CGCC
NdeI 371 CATATG CA^TATG
PflMI 741 CCAAATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 526 GACTC GAGTC(4/5)
SbfI 727 CCTGCAGG CCTGCA^GG
SfoI 193 GGCGCC GGC^GCC
SphI 366 GCATGC GCATG^C
Sse8387I 727 CCTGCAGG CCTGCA^GG
Tsp45I 79 GTGAC ^GT[GC]AC
Tsp509I 498 AATT ^AATT
Van91I 741 CCAAATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 430 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AlwI 553 GATCC GGATC(4/5)
430 GGATC
AvaII 724 GGTCC G^G[AT]CC
842 GGTCC
BanI 121 GGCACC G^G[CT][GA]CC
193 GGCGCC
BclI 393 TGATCA T^GATCA
501 TGATCA
BcnI 4 CCGGG CC^[GC]GG
697 CCCGG
BfaI 379 CTAG C^TAG
517 CTAG
BmyI 120 GGGCAC G[AGT]GC[ACT]^C
353 GTGCTC
BsaAI 464 CACGTA [CT]AC^GT[GA]
811 TACGTG
Bsp1286I 120 GGGCAC G[AGT]GC[ACT]^C
353 GTGCTC
BsrBI 282 CCGCTC CCGCTC(-3/-3)
69 GAGCGG
BstF5I 475 GGATG GGATG(2/0)
597 GGATG
Cfr13I 724 GGTCC G^G[ACGT]CC
842 GGTCC
EcoT22I 364 ATGCAT ATGCA^T
368 ATGCAT
FauI 59 GCGGG CCCGC(4/6)
71 GCGGG
FbaI 393 TGATCA T^GATCA
501 TGATCA
FokI 475 GGATG GGATG(9/13)
597 GGATG
HaeII 193 GGCGCC [GA]GCGC^[CT]
675 GGCGCT
HphI 78 GGTGA GGTGA(8/7)
804 GGTGA
MaeI 379 CTAG C^TAG
517 CTAG
MaeII 465 ACGT A^CGT
812 ACGT
NciI 4 CCGGG CC^[GC]GG
697 CCCGG
NlaIV 121 GGCACC GG[ACGT]^[ACGT]CC
193 GGCGCC
NsiI 364 ATGCAT ATGCA^T
368 ATGCAT
PstI 653 CTGCAG CTGCA^G
728 CTGCAG
Sau96I 724 GGTCC G^G[ACGT]CC
842 GGTCC
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# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AccII AciI AfaI AluI BbvI
BsiYI BslI BssKI BstNI BstUI
Cac8I CfoI DpnII EcoRII Fnu4HI
HapII HhaI HinP1I HinfI HpaII
ItaI MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NdeII NlaIII NspI
RsaI Sau3AI ScrFI SfaNI SfcI
TfiI Tru9I TseI TspRI