(Circular) (Six-base) MAPSORT of: pFN21AE1777.seq Check: 6299 from: 1 to: 5560 pFN21AE1777 5560 bp With 182 enzymes: SgfI * March 28, 2012 12:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3370 278 Size: 53 83 186 936 1834 2468 Fragments arranged by size: 2468 1834 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2903 1937 Size: 966 4594 Acc65I G'GTAC_C Cuts at: 2881 2881 Size: 5560 AclI AA'CG_TT Cuts at: 1563 3687 4060 1563 Size: 2124 373 3063 Fragments arranged by size: 3063 2124 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2162 2559 2643 3623 5007 5275 1632 Size: 530 397 84 980 1384 268 1917 Fragments arranged by size: 1917 1384 980 530 397 268 84 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5363 AflIII A'CryG_T Cuts at: 1203 4459 5436 1203 Size: 3256 977 1327 Fragments arranged by size: 3256 1327 977 AgeI A'CCGG_T Cuts at: 4391 4391 Size: 5560 AhdI GACnn_n'nnGTC Cuts at: 4289 4289 Size: 5560 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5560 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2394 1184 Size: 1210 4350 AlwNI CAG_nnn'CTG Cuts at: 2666 3303 4875 2666 Size: 637 1572 3351 Fragments arranged by size: 3351 1572 637 ApaLI G'TGCA_C Cuts at: 3617 4773 3617 Size: 1156 4404 ApoI r'AATT_y Cuts at: 1493 2869 3095 3247 5356 1493 Size: 1376 226 152 2109 1697 Fragments arranged by size: 2109 1697 1376 226 152 AseI AT'TA_AT Cuts at: 160 1032 4114 160 Size: 872 3082 1606 Fragments arranged by size: 3082 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5560 AvaI C'yCGr_G Cuts at: 1870 1943 2885 1870 Size: 73 942 4545 Fragments arranged by size: 4545 942 73 BamHI G'GATC_C Cuts at: 2890 3257 2890 Size: 367 5193 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2126 2198 2881 4336 618 Size: 325 217 627 339 72 683 1455 1842 Fragments arranged by size: 1842 1455 683 627 339 325 217 72 BanII G_rGCy'C Cuts at: 1391 2879 1391 Size: 1488 4072 BbeI G_GCGC'C Cuts at: 2202 2202 Size: 5560 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2565 928 Size: 971 666 3923 Fragments arranged by size: 3923 971 666 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2116 2150 3751 3785 1903 Size: 34 50 34 95 34 1601 34 3678 Fragments arranged by size: 3678 1601 95 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3452 4662 3452 Size: 1210 4350 BclI T'GATC_A Cuts at: 1548 2398 2506 1548 Size: 850 108 4602 Fragments arranged by size: 4602 850 108 BfrBI ATG'CAT Cuts at: 2371 2375 2371 Size: 4 5556 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4171 136 Size: 107 122 71 631 605 2499 1525 Fragments arranged by size: 2499 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5555 5555 Size: 5560 BlpI GC'TnA_GC Cuts at: 2173 2977 2173 Size: 804 4756 Bme1580I G_kGCm'C Cuts at: 1790 2129 3621 4777 1790 Size: 339 1492 1156 2573 Fragments arranged by size: 2573 1492 1156 339 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5560 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3291 4249 442 Size: 416 53 83 399 24 1874 958 1753 Fragments arranged by size: 1874 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5560 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2119 2119 Size: 5560 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4220 1239 Size: 2981 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3685 4550 4848 3685 Size: 865 298 4397 Fragments arranged by size: 4397 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2107 4223 882 Size: 617 608 2116 2219 Fragments arranged by size: 2219 2116 617 608 BsaAI yAC'GTr Cuts at: 493 1204 2471 2818 3351 493 Size: 711 1267 347 533 2702 Fragments arranged by size: 2702 1267 711 533 347 BsaBI GATnn'nnATC Cuts at: 5554 5554 Size: 5560 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2199 3367 3749 4384 Size: 53 83 186 936 666 1168 382 635 Cuts at: 4384 275 Size: 1451 Fragments arranged by size: 1451 1168 936 666 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3993 4391 4665 4812 5223 3993 Size: 398 274 147 411 4330 Fragments arranged by size: 4330 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5335 Fragments arranged by size: 5335 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5560 BseYI C'CCAG_C Cuts at: 2122 4763 5259 2122 Size: 2641 496 2423 Fragments arranged by size: 2641 2423 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4987 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3010 3771 4799 1540 Size: 275 186 1009 761 1028 2301 Fragments arranged by size: 2301 1028 1009 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2362 3621 3706 4777 2362 Size: 1259 85 1071 3145 Fragments arranged by size: 3145 1259 1071 85 BsmI GAATG_Cn' Cuts at: 3071 3164 3071 Size: 93 5467 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2129 2362 2879 3621 3706 4777 1391 Size: 399 339 233 517 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 517 399 339 233 85 BspEI T'CCGG_A Cuts at: 5223 5223 Size: 5560 BspHI T'CATG_A Cuts at: 3449 5179 3449 Size: 1730 3830 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2166 2915 844 Size: 1322 749 3489 Fragments arranged by size: 3489 1322 749 BsrBI CCG'CTC Cuts at: 1394 1823 2076 2289 3447 1394 Size: 429 253 213 1158 3507 Fragments arranged by size: 3507 1158 429 253 213 BsrDI GCAATG_nn' Cuts at: 65 1248 4055 4229 65 Size: 1183 2807 174 1396 Fragments arranged by size: 2807 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4204 4391 1700 Size: 110 2394 187 2869 Fragments arranged by size: 2869 2394 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5560 BssHII G'CGCG_C Cuts at: 1925 2015 1925 Size: 90 5470 BssSI C'ACGA_G Cuts at: 3620 4632 3620 Size: 1012 4548 BstAPI GCAn_nnn'nTGC Cuts at: 794 2465 794 Size: 1671 3889 BstBI TT'CG_AA Cuts at: 4364 4364 Size: 5560 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5560 BstXI CCAn_nnnn'nTGG Cuts at: 2650 2650 Size: 5560 BstYI r'GATC_y Cuts at: 1972 2435 2890 3257 3642 3659 5111 5197 5209 Size: 463 455 367 385 17 1452 86 12 Cuts at: 5209 5555 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 463 455 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5008 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2192 487 Size: 683 56 966 3855 Fragments arranged by size: 3855 966 683 56 BtsI GCAGTG_nn' Cuts at: 2026 2849 3076 3870 3890 2026 Size: 823 227 794 20 3696 Fragments arranged by size: 3696 823 794 227 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5560 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5525 DraI TTT'AAA Cuts at: 2864 2864 Size: 5560 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4567 809 Size: 507 3251 1802 Fragments arranged by size: 3251 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3007 3896 5525 Size: 54 1425 50 275 18 1177 889 1629 Cuts at: 5525 8 Size: 43 Fragments arranged by size: 1629 1425 1177 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3007 1537 Size: 275 1195 4090 Fragments arranged by size: 4090 1195 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3490 1353 Size: 2137 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2754 4147 4521 4667 387 Size: 1064 138 1165 1393 374 146 1280 Fragments arranged by size: 1393 1280 1165 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2162 2559 2643 3623 4220 5007 5275 Size: 393 530 397 84 980 597 787 268 Cuts at: 5275 1239 Size: 1524 Fragments arranged by size: 1524 980 787 597 530 397 393 268 84 EcoRI G'AATT_C Cuts at: 2869 3247 2869 Size: 378 5182 FspI TGC'GCA Cuts at: 4066 4066 Size: 5560 HaeII r_GCGC'y Cuts at: 1678 2202 2684 4707 1678 Size: 524 482 2023 2531 Fragments arranged by size: 2531 2023 524 482 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2420 2452 4201 4233 4275 Size: 32 135 32 848 32 1749 32 42 Cuts at: 4275 4307 1373 Size: 32 2626 Fragments arranged by size: 2626 1749 848 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2904 3150 669 Size: 988 281 966 246 3079 Fragments arranged by size: 3079 988 966 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5560 HpaI GTT'AAC Cuts at: 3150 3150 Size: 5560 KasI G'GCGC_C Cuts at: 2198 2198 Size: 5560 KpnI G_GTAC'C Cuts at: 2885 2885 Size: 5560 MfeI C'AATT_G Cuts at: 3159 3159 Size: 5560 MluI A'CGCG_T Cuts at: 5436 5436 Size: 5560 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2458 2585 2733 4674 4858 1305 Size: 1153 127 148 1941 184 2007 Fragments arranged by size: 2007 1941 1153 184 148 127 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3738 Fragments arranged by size: 3738 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2370 2376 2648 2789 3518 3877 4036 Size: 648 1204 6 272 141 729 359 159 Cuts at: 4036 518 Size: 2042 Fragments arranged by size: 2042 1204 729 648 359 272 159 141 6 MspA1I CmG'CkG Cuts at: 1606 2031 2052 2064 2976 3653 4801 5046 1606 Size: 425 21 12 912 677 1148 245 2120 Fragments arranged by size: 2120 1148 912 677 425 245 21 12 NarI GG'CG_CC Cuts at: 2199 2199 Size: 5560 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5008 NdeI CA'TA_TG Cuts at: 387 2377 387 Size: 1990 3570 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5560 NotI GC'GGCC_GC Cuts at: 3007 3007 Size: 5560 NsiI A_TGCA'T Cuts at: 2373 2377 2373 Size: 4 5556 NspI r_CATG'y Cuts at: 1132 1229 2371 2375 2463 2918 4463 1132 Size: 97 1142 4 88 455 1545 2229 Fragments arranged by size: 2229 1545 1142 455 97 88 4 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5560 PciI A'CATG_T Cuts at: 4459 4459 Size: 5560 PflMI CCAn_nnn'nTGG Cuts at: 1108 2752 1108 Size: 1644 3916 PmeI GTTT'AAAC Cuts at: 2864 2864 Size: 5560 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3629 3661 5142 5174 3629 Size: 32 1481 32 4015 Fragments arranged by size: 4015 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5560 PsiI TTA'TAA Cuts at: 3130 3130 Size: 5560 PstI C_TGCA'G Cuts at: 830 2662 2737 2912 830 Size: 1832 75 175 3478 Fragments arranged by size: 3478 1832 175 75 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5560 SalI G'TCGA_C Cuts at: 1936 2902 1936 Size: 966 4594 SbfI CC_TGCA'GG Cuts at: 2737 2912 2737 Size: 175 5385 ScaI AGT'ACT Cuts at: 3808 5520 3808 Size: 1712 3848 SfcI C'TryA_G Cuts at: 826 1045 2059 2280 2658 2733 2908 4043 4724 Size: 219 1014 221 378 75 175 1135 681 Cuts at: 4724 4915 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1014 681 378 221 219 191 175 75 SfoI GGC'GCC Cuts at: 2200 2200 Size: 5560 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5560 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5560 SmaI CCC'GGG Cuts at: 1872 2887 1872 Size: 1015 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3664 4565 4827 820 Size: 197 926 1721 901 262 1553 Fragments arranged by size: 1721 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3351 493 Size: 2858 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5560 SphI G_CATG'C Cuts at: 1132 2375 2918 1132 Size: 1243 543 3774 Fragments arranged by size: 3774 1243 543 SspI AAT'ATT Cuts at: 5 52 3484 5 Size: 47 3432 2081 Fragments arranged by size: 3432 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5560 StyI C'CwwG_G Cuts at: 513 1065 2999 513 Size: 552 1934 3074 Fragments arranged by size: 3074 1934 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3587 3604 3757 3942 4387 325 Size: 1255 2007 17 153 185 445 1498 Fragments arranged by size: 2007 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2483 2584 3806 Size: 274 80 33 51 1443 506 101 1222 Cuts at: 3806 5518 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1222 506 274 138 101 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5520 5552 5520 Size: 32 5528 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5560 XbaI T'CTAG_A Cuts at: 2896 2896 Size: 5560 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5560 XmaI C'CCGG_G Cuts at: 1870 2885 1870 Size: 1015 4545 XmnI GAAnn'nnTTC Cuts at: 3689 3689 Size: 5560 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3368 276 Size: 53 83 186 936 1834 2468 Fragments arranged by size: 2468 1834 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI