################################## # NO CUT ENZYMES ( 134 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI Alw44I AlwNI Aor51HI ApaLI AscI AseI AsnI Asp718I AvaI AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaI BseAI BsgI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstYI BstZ17I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KasI KpnI KspI MaeII MamI MflI MluI MroI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SwaI XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AcyI 124 GACGCC G[GA]^CG[CT]C AhdI 892 GACAAACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 664 CCTGAGG CC^T[ACGT]AGG Asp700I 553 GAATGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 892 GACAAACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 1230 TGGCCA TGG^CCA BsaHI 124 GACGCC G[GA]^CG[CT]C BsaWI 699 ACCGGA [AT]^CCGG[AT] BsmAI 973 GAGAC GTCTC(1/5) BsmI 450 GAATGC GAATGC(1/-1) BspHI 405 TCATGA T^CATGA BsrBI 565 CCGCTC CCGCTC(-3/-3) BsrDI 626 CATTGC GCAATG(2/0) Bsu36I 664 CCTGAGG CC^T[ACGT]AGG DsaI 1278 CCATGG C^C[GA][CT]GG EaeI 1230 TGGCCA [CT]^GGCC[GA] Eam1105I 892 GACAAACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 568 CTCTTC CTCTTC(1/4) Eco81I 664 CCTGAGG CC^T[ACGT]AGG HaeII 88 AGCGCC [GA]GCGC^[CT] HapII 700 CCGG C^CGG HgaI 124 GACGC GACGC(5/10) Hin1I 124 GACGCC G[GA]^CG[CT]C HpaII 700 CCGG C^CGG Ksp632I 568 CTCTTC CTCTTC(1/4) MfeI 944 CAATTG C^AATTG MluNI 1230 TGGCCA TGG^CCA MscI 1230 TGGCCA TGG^CCA MslI 863 CACATAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 700 CCGG C^CGG MunI 944 CAATTG C^AATTG NcoI 1278 CCATGG C^CATGG PflMI 341 CCAACCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 115 GAGTC GAGTC(4/5) PpuMI 1069 GGGTCCT [GA]G^G[AT]CC[CT] PstI 828 CTGCAG CTGCA^G RcaI 405 TCATGA T^CATGA SapI 567 GCTCTTC GCTCTTC(1/4) StuI 365 AGGCCT AGG^CCT TaqI 101 TCGA T^CGA TthHB8I 101 TCGA T^CGA Van91I 341 CCAACCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1238 CCAAAGCTATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 553 GAATGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AcsI 262 GAATTT [GA]^AATT[CT] 328 AAATTT AfaI 537 GTAC GT^AC 1155 GTAC AlwI 1121 GATCC GGATC(4/5) 702 GGATC ApaI 466 GGGCCC GGGCC^C 1012 GGGCCC ApoI 262 GAATTT [GA]^AATT[CT] 328 AAATTT AspI 478 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1132 GACACTGTC AvaII 310 GGACC G^G[AT]CC 1070 GGTCC BanI 373 GGTGCC G^G[CT][GA]CC 1027 GGCACC BseRI 692 GAGGAG GAGGAG(10/8) 1048 GAGGAG BsmFI 476 GGGAC GGGAC(10/14) 117 GTCCC BspMI 521 ACCTGC ACCTGC(4/8) 826 ACCTGC CfoI 89 GCGC GCG^C 96 GCGC DraII 465 GGGGCCC [GA]G^G[ACGT]CC[CT] 1069 GGGTCCT EcoO109I 465 GGGGCCC [GA]G^G[ACGT]CC[CT] 1069 GGGTCCT EcoT14I 1227 CCTTGG C^C[AT][AT]GG 1278 CCATGG FauI 128 CCCGC CCCGC(4/6) 139 CCCGC HhaI 89 GCGC GCG^C 96 GCGC HinP1I 89 GCGC G^CGC 96 GCGC MspA1I 133 CAGCTG C[AC]G^C[GT]G 1219 CAGCTG PflFI 478 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1132 GACACTGTC PvuII 133 CAGCTG CAG^CTG 1219 CAGCTG RsaI 537 GTAC GT^AC 1155 GTAC SacI 13 GAGCTC GAGCT^C 634 GAGCTC SmlI 493 CTTGAG C^T[CT][GA]AG 574 CTTGAG SspI 434 AATATT AAT^ATT 1006 AATATT StyI 1227 CCTTGG C^C[AT][AT]GG 1278 CCATGG TfiI 583 GAATC G^A[AT]TC 926 GAATC Tth111I 478 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1132 GACACTGTC ######################################### # 3 OR MORE CUT ENZYMES ( 52 enzymes) # ######################################### AccII AciI AluI AspHI BanII BbvI BfaI BmyI BsaJI BsiHKAI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstXI Cac8I Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI MaeI MaeIII MboI MboII MnlI MseI MvaI MvnI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI SfcI Tru9I TseI Tsp45I Tsp509I TspRI