(Linear) (Six-base) MAPSORT of: KIEE5533.seq Check: 5097 from: 1 to: 1401 KIEE5533.orf With 182 enzymes: SgfI * January 29, 2014 12:04 .. AhdI GACnn_n'nnGTC Cuts at: 0 897 1401 Size: 897 504 ApaI G_GGCC'C Cuts at: 0 470 1016 1401 Size: 470 546 385 Fragments arranged by size: 546 470 385 ApoI r'AATT_y Cuts at: 0 262 328 1401 Size: 262 66 1073 Fragments arranged by size: 1073 262 66 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 238 271 1401 Size: 238 33 1130 Fragments arranged by size: 1130 238 33 BanI G'GyrC_C Cuts at: 0 373 1027 1401 Size: 373 654 374 Fragments arranged by size: 654 374 373 BanII G_rGCy'C Cuts at: 0 17 470 638 1016 1380 1401 Size: 17 453 168 378 364 21 Fragments arranged by size: 453 378 364 168 21 17 Bme1580I G_kGCm'C Cuts at: 0 79 200 470 766 1016 1401 Size: 79 121 270 296 250 385 Fragments arranged by size: 385 296 270 250 121 79 BmrI ACTGGGnnnn_n' Cuts at: 0 189 748 1282 1401 Size: 189 559 534 119 Fragments arranged by size: 559 534 189 119 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 514 595 1401 Size: 514 81 806 Fragments arranged by size: 806 514 81 BsaHI Gr'CG_yC Cuts at: 0 125 1401 Size: 125 1276 BsaWI w'CCGG_w Cuts at: 0 699 1401 Size: 699 702 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 707 1063 1401 Size: 707 356 338 Fragments arranged by size: 707 356 338 BseYI C'CCAG_C Cuts at: 0 199 1373 1401 Size: 199 1174 28 Fragments arranged by size: 1174 199 28 BsiHKAI G_wGCw'C Cuts at: 0 17 638 699 1401 Size: 17 621 61 702 Fragments arranged by size: 702 621 61 17 BsmI GAATG_Cn' Cuts at: 0 456 1401 Size: 456 945 Bsp1286I G_dGCh'C Cuts at: 0 17 79 200 470 638 699 766 1016 Size: 17 62 121 270 168 61 67 250 Cuts at: 1016 1380 1401 Size: 364 21 Fragments arranged by size: 364 270 250 168 121 67 62 61 21 17 BspHI T'CATG_A Cuts at: 0 405 1401 Size: 405 996 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 530 835 1401 Size: 530 305 566 Fragments arranged by size: 566 530 305 BsrBI CCG'CTC Cuts at: 0 567 1401 Size: 567 834 BsrDI GCAATG_nn' Cuts at: 0 625 1401 Size: 625 776 BstXI CCAn_nnnn'nTGG Cuts at: 0 869 1151 1295 1401 Size: 869 282 144 106 Fragments arranged by size: 869 282 144 106 Bsu36I CC'TnA_GG Cuts at: 0 665 1401 Size: 665 736 BtgI C'CryG_G Cuts at: 0 1278 1401 Size: 1278 123 BtsI GCAGTG_nn' Cuts at: 0 837 1401 Size: 837 564 EaeI y'GGCC_r Cuts at: 0 1230 1401 Size: 1230 171 EarI CTCTTCn'nnn_ Cuts at: 0 574 1401 Size: 574 827 EcoICRI GAG'CTC Cuts at: 0 15 636 1401 Size: 15 621 765 Fragments arranged by size: 765 621 15 EcoO109I rG'GnC_Cy Cuts at: 0 466 1070 1401 Size: 466 604 331 Fragments arranged by size: 604 466 331 HaeII r_GCGC'y Cuts at: 0 92 1401 Size: 92 1309 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 568 600 883 915 1401 Size: 568 32 283 32 486 Fragments arranged by size: 568 486 283 32 32 MfeI C'AATT_G Cuts at: 0 944 1401 Size: 944 457 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 659 1401 Size: 659 742 MscI TGG'CCA Cuts at: 0 1232 1401 Size: 1232 169 MslI CAynn'nnrTG Cuts at: 0 867 1401 Size: 867 534 MspA1I CmG'CkG Cuts at: 0 135 1221 1401 Size: 135 1086 180 Fragments arranged by size: 1086 180 135 NcoI C'CATG_G Cuts at: 0 1278 1401 Size: 1278 123 PflMI CCAn_nnn'nTGG Cuts at: 0 347 1401 Size: 347 1054 PpuMI rG'GwC_Cy Cuts at: 0 1070 1401 Size: 1070 331 PsiI TTA'TAA Cuts at: 0 842 1401 Size: 842 559 PspOMI G'GGCC_C Cuts at: 0 466 1012 1401 Size: 466 546 389 Fragments arranged by size: 546 466 389 PstI C_TGCA'G Cuts at: 0 832 1401 Size: 832 569 PvuII CAG'CTG Cuts at: 0 135 1221 1401 Size: 135 1086 180 Fragments arranged by size: 1086 180 135 SacI G_AGCT'C Cuts at: 0 17 638 1401 Size: 17 621 763 Fragments arranged by size: 763 621 17 SapI GCTCTTCn'nnn_ Cuts at: 0 574 1401 Size: 574 827 SfcI C'TryA_G Cuts at: 0 828 909 1037 1401 Size: 828 81 128 364 Fragments arranged by size: 828 364 128 81 SmlI C'TyrA_G Cuts at: 0 493 574 1401 Size: 493 81 827 Fragments arranged by size: 827 493 81 SspI AAT'ATT Cuts at: 0 436 1008 1401 Size: 436 572 393 Fragments arranged by size: 572 436 393 StuI AGG'CCT Cuts at: 0 367 1401 Size: 367 1034 StyI C'CwwG_G Cuts at: 0 1227 1278 1401 Size: 1227 51 123 Fragments arranged by size: 1227 123 51 TatI w'GTAC_w Cuts at: 0 536 1401 Size: 536 865 Tth111I GACn'n_nGTC Cuts at: 0 481 1135 1401 Size: 481 654 266 Fragments arranged by size: 654 481 266 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1245 1401 Size: 1245 156 XmnI GAAnn'nnTTC Cuts at: 0 557 1401 Size: 557 844 Enzymes that do cut: AhdI ApaI ApoI BaeI BanI BanII Bme1580I BmrI BpuEI BsaHI BsaWI BseRI BseYI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BstXI Bsu36I BtgI BtsI EaeI EarI EcoICRI EcoO109I HaeII Hin4I MfeI MmeI MscI MslI MspA1I NcoI PflMI PpuMI PsiI PspOMI PstI PvuII SacI SapI SfcI SmlI SspI StuI StyI TatI Tth111I XcmI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AleI AlfI AloI AlwNI ApaLI AscI AseI AsiSI AvaI AvrII BamHI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI BmtI BplI BpmI Bpu10I BsaI BsaAI BsaBI BsaXI BsgI BsiEI BsiWI BsmBI BspEI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI Eco57MI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KasI KpnI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PfoI PmeI PmlI PpiI PshAI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI TaqII TstI XbaI XhoI XmaI ZraI