################################## # NO CUT ENZYMES ( 120 enzymes) # ################################## AccII AccIII AclI AcsI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspI AvaI AviII AvrII BamHI BbeI BbrPI BbsI BcgI BciVI BcnI BfrI BglI BlnI BpuAI BsaAI BseAI BsgI BsiEI BsiWI BsoBI BspDI BspEI BspMI BsrBI BssHII BssSI Bst1107I BstBI BstUI BstZ17I Bsu36I CfoI ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV FauI FseI FspI HaeII HhaI HinP1I HpaI KasI KspI MluI MroI MvnI NarI NciI NdeI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SphI Sse8387I SspI SwaI TaqI Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### AatII 1363 GACGTC GACGT^C Acc65I 3 GGTACC G^GTACC AccI 1012 GTAGAC GT^[AC][GT]AC AcyI 1363 GACGTC G[GA]^CG[CT]C AflIII 593 ACATGT A^C[GA][CT]GT AlwNI 1352 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 24 GGGCCC GGGCC^C Asp718I 3 GGTACC G^GTACC BalI 876 TGGCCA TGG^CCA BanI 3 GGTACC G^G[CT][GA]CC BbvCI 1298 CCTCAGC CCTCAGC(-5/-2) BclI 755 TGATCA T^GATCA BglII 1466 AGATCT A^GATCT BlpI 1458 GCTCAGC GC^T[ACGT]AGC BpmI 1841 CTCCAG CTGGAG(16/14) Bpu1102I 1458 GCTCAGC GC^T[ACGT]AGC BsaHI 1363 GACGTC G[GA]^CG[CT]C BsaWI 286 ACCGGA [AT]^CCGG[AT] BsmI 533 GCATTC GAATGC(1/-1) Bsp1407I 864 TGTACA T^GTACA BspHI 1607 TCATGA T^CATGA BspLU11I 593 ACATGT A^CATGT BsrFI 80 GCCGGC [GA]^CCGG[CT] BsrGI 864 TGTACA T^GTACA BstAPI 466 GCAGAGATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1074 GGTGACC G^GT[ACGT]ACC BstPI 1074 GGTGACC G^GT[ACGT]ACC BstXI 1441 CCAATGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 1458 GCTCAGC GC^T[ACGT]AGC Cfr10I 80 GCCGGC [GA]^CCGG[CT] EaeI 876 TGGCCA [CT]^GGCC[GA] EcoO65I 1074 GGTGACC G^GT[ACGT]ACC EcoT22I 899 ATGCAT ATGCA^T FbaI 755 TGATCA T^GATCA HgaI 407 GACGC GACGC(5/10) Hin1I 1363 GACGTC G[GA]^CG[CT]C HincII 1191 GTCAAC GT[CT]^[GA]AC HindII 1191 GTCAAC GT[CT]^[GA]AC HindIII 360 AAGCTT A^AGCTT KpnI 3 GGTACC GGTAC^C MaeII 1364 ACGT A^CGT MluNI 876 TGGCCA TGG^CCA MscI 876 TGGCCA TGG^CCA MspA1I 72 CAGCGG C[AC]G^C[GT]G NaeI 80 GCCGGC GCC^GGC NgoMIV 80 GCCGGC G^CCGGC NsiI 899 ATGCAT ATGCA^T PflMI 1149 CCAGTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 293 GGGTCCT [GA]G^G[AT]CC[CT] PstI 1125 CTGCAG CTGCA^G RcaI 1607 TCATGA T^CATGA SacI 249 GAGCTC GAGCT^C SapI 190 GCTCTTC GCTCTTC(1/4) SfcI 1125 CTGCAG C^T[GA][CT]AG SpeI 852 ACTAGT A^CTAGT SspBI 864 TGTACA T^GTACA StuI 492 AGGCCT AGG^CCT TfiI 609 GATTC G^A[AT]TC Van91I 1149 CCAGTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### BsaBI 522 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 775 GATGAATATC BsmBI 61 GAGACG CGTCTC(1/5) 1291 GAGACG BstYI 798 GGATCT [GA]^GATC[CT] 1466 AGATCT HapII 81 CCGG C^CGG 287 CCGG HpaII 81 CCGG C^CGG 287 CCGG MamI 522 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 775 GATGAATATC MfeI 632 CAATTG C^AATTG 1427 CAATTG MflI 798 GGATCT [GA]^GATC[CT] 1466 AGATCT MslI 1101 CATCCACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1583 CATGGGTGTG MspI 81 CCGG C^CGG 287 CCGG MunI 632 CAATTG C^AATTG 1427 CAATTG XhoII 798 GGATCT [GA]^GATC[CT] 1466 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 67 enzymes) # ######################################### AciI AfaI AluI AlwI AspHI AvaII BanII BbvI BfaI BmyI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraII DsaI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MseI MvaI MwoI NcoI NdeII NheI NlaIII NlaIV NspI PleI RsaI Sau3AI Sau96I ScrFI SfaNI StyI Tru9I TseI Tsp45I Tsp509I TspRI