(Circular) (Six-base) MAPSORT of: pFN21AE6494.seq Check: 4279 from: 1 to: 6556 pFN21AE6494 6556 bp With 182 enzymes: SgfI * March 18, 2015 14:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3372 4366 278 Size: 53 83 186 936 1836 994 2468 Fragments arranged by size: 2468 1836 994 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3018 3899 1937 Size: 1081 881 4594 Fragments arranged by size: 4594 1081 881 Acc65I G'GTAC_C Cuts at: 2008 3877 2008 Size: 1869 4687 AclI AA'CG_TT Cuts at: 1563 4683 5056 1563 Size: 3120 373 3063 Fragments arranged by size: 3120 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2186 4619 6003 6271 1632 Size: 554 2433 1384 268 1917 Fragments arranged by size: 2433 1917 1384 554 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6359 AflIII A'CryG_T Cuts at: 1203 2598 5455 6432 1203 Size: 1395 2857 977 1327 Fragments arranged by size: 2857 1395 1327 977 AgeI A'CCGG_T Cuts at: 5387 5387 Size: 6556 AhdI GACnn_n'nnGTC Cuts at: 5285 5285 Size: 6556 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6556 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2655 1184 Size: 1471 5085 AlwNI CAG_nnn'CTG Cuts at: 3362 4299 5871 3362 Size: 937 1572 4047 Fragments arranged by size: 4047 1572 937 ApaI G_GGCC'C Cuts at: 2033 2033 Size: 6556 ApaLI G'TGCA_C Cuts at: 4613 5769 4613 Size: 1156 5400 ApoI r'AATT_y Cuts at: 1493 3865 4091 4243 6352 1493 Size: 2372 226 152 2109 1697 Fragments arranged by size: 2372 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5110 160 Size: 872 4078 1606 Fragments arranged by size: 4078 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6556 AvaI C'yCGr_G Cuts at: 1870 1943 3881 1870 Size: 73 1938 4545 Fragments arranged by size: 4545 1938 73 BamHI G'GATC_C Cuts at: 3886 4253 3886 Size: 367 6189 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2008 3877 5332 618 Size: 325 217 627 221 1869 1455 1842 Fragments arranged by size: 1869 1842 1455 627 325 221 217 BanII G_rGCy'C Cuts at: 1391 2033 2197 2258 2275 3875 1391 Size: 642 164 61 17 1600 4072 Fragments arranged by size: 4072 1600 642 164 61 17 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5585 BbvCI CC'TCA_GC Cuts at: 3304 3304 Size: 6556 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4747 4781 1903 Size: 34 2810 34 3678 Fragments arranged by size: 3678 2810 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4448 5658 4448 Size: 1210 5346 BclI T'GATC_A Cuts at: 1548 2760 1548 Size: 1212 5344 BfrBI ATG'CAT Cuts at: 2906 2906 Size: 6556 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5167 136 Size: 107 122 71 631 605 3495 1525 Fragments arranged by size: 3495 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3471 6551 3471 Size: 3080 3476 BlpI GC'TnA_GC Cuts at: 3464 3973 3464 Size: 509 6047 Bme1580I G_kGCm'C Cuts at: 1790 2033 4617 5773 1790 Size: 243 2584 1156 2573 Fragments arranged by size: 2584 2573 1156 243 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6556 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2028 2976 3535 Size: 416 53 83 399 24 611 948 559 Cuts at: 3535 4287 5245 442 Size: 752 958 1753 Fragments arranged by size: 1753 958 948 752 611 559 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2706 2955 3698 1056 Size: 1650 249 743 3914 Fragments arranged by size: 3914 1650 743 249 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3319 3319 Size: 6556 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3831 5216 1239 Size: 2592 1385 2579 Fragments arranged by size: 2592 2579 1385 Bpu10I CC'TnA_GC Cuts at: 2225 3304 3397 2225 Size: 1079 93 5384 Fragments arranged by size: 5384 1079 93 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4681 5546 5844 4681 Size: 865 298 5393 Fragments arranged by size: 5393 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2434 3213 3845 5219 882 Size: 617 935 779 632 1374 2219 Fragments arranged by size: 2219 1374 935 779 632 617 BsaAI yAC'GTr Cuts at: 493 1204 4347 493 Size: 711 3143 2702 Fragments arranged by size: 3143 2702 711 BsaBI GATnn'nnATC Cuts at: 2531 2784 6550 2531 Size: 253 3766 2537 Fragments arranged by size: 3766 2537 253 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3369 4363 4745 5380 Size: 53 83 186 936 1836 994 382 635 Cuts at: 5380 275 Size: 1451 Fragments arranged by size: 1836 1451 994 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2291 4989 5387 5661 5808 6219 2291 Size: 2698 398 274 147 411 2628 Fragments arranged by size: 2698 2628 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6331 Fragments arranged by size: 6331 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2472 3046 3530 1186 Size: 1286 574 484 4212 Fragments arranged by size: 4212 1286 574 484 BseYI C'CCAG_C Cuts at: 3373 5759 6255 3373 Size: 2386 496 3674 Fragments arranged by size: 3674 2386 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5983 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4006 4767 5795 1540 Size: 275 186 2005 761 1028 2301 Fragments arranged by size: 2301 2005 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2258 2731 3465 4617 4702 5773 2258 Size: 473 734 1152 85 1071 3041 Fragments arranged by size: 3041 1152 1071 734 473 85 BsmI GAATG_Cn' Cuts at: 2538 4067 4160 2538 Size: 1529 93 4934 Fragments arranged by size: 4934 1529 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2060 3290 2060 Size: 1230 5326 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2033 2197 2258 2275 2731 3465 3875 Size: 399 243 164 61 17 456 734 410 Cuts at: 3875 4617 4702 5773 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 734 456 410 399 243 164 85 61 17 BspEI T'CCGG_A Cuts at: 6219 6219 Size: 6556 BspHI T'CATG_A Cuts at: 3612 4445 6175 3612 Size: 833 1730 3993 Fragments arranged by size: 3993 1730 833 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3911 844 Size: 3067 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4443 1394 Size: 429 2620 3507 Fragments arranged by size: 3507 2620 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2415 2448 2649 2947 5051 5225 65 Size: 1183 1167 33 201 298 2104 174 1396 Fragments arranged by size: 2104 1396 1183 1167 298 201 174 33 BsrFI r'CCGG_y Cuts at: 1700 1810 2085 5200 5387 1700 Size: 110 275 3115 187 2869 Fragments arranged by size: 3115 2869 275 187 110 BsrGI T'GTAC_A Cuts at: 96 2869 96 Size: 2773 3783 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6556 BssSI C'ACGA_G Cuts at: 4616 5628 4616 Size: 1012 5544 BstAPI GCAn_nnn'nTGC Cuts at: 794 2477 794 Size: 1683 4873 BstBI TT'CG_AA Cuts at: 5360 5360 Size: 6556 BstEII G'GTnAC_C Cuts at: 1184 3079 1184 Size: 1895 4661 BstXI CCAn_nnnn'nTGG Cuts at: 3453 3453 Size: 6556 BstYI r'GATC_y Cuts at: 1972 2803 3471 3886 4253 4638 4655 6107 6193 Size: 831 668 415 367 385 17 1452 86 Cuts at: 6193 6205 6551 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 831 668 415 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2061 2584 3084 3501 3587 513 Size: 552 939 57 523 500 417 86 3482 Fragments arranged by size: 3482 939 552 523 500 417 86 57 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2452 487 Size: 683 56 1226 4591 Fragments arranged by size: 4591 1226 683 56 BtsI GCAGTG_nn' Cuts at: 2139 4072 4866 4886 2139 Size: 1933 794 20 3809 Fragments arranged by size: 3809 1933 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6556 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2953 2988 2995 3030 3799 3834 529 Size: 35 2389 35 7 35 769 35 3251 Fragments arranged by size: 3251 2389 769 35 35 35 35 7 DraI TTT'AAA Cuts at: 3860 3860 Size: 6556 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5563 809 Size: 507 4247 1802 Fragments arranged by size: 4247 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2881 4003 4892 Size: 54 1425 50 275 18 1051 1122 889 Cuts at: 4892 6521 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1122 1051 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4003 1537 Size: 275 2191 4090 Fragments arranged by size: 4090 2191 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2202 2449 2488 3015 4486 1353 Size: 849 247 39 527 1471 3423 Fragments arranged by size: 3423 1471 849 527 247 39 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5143 5517 5663 387 Size: 1064 138 3554 374 146 1280 Fragments arranged by size: 3554 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2256 2256 Size: 6556 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2186 3831 4619 5216 6003 6271 1239 Size: 393 554 1645 788 597 787 268 1524 Fragments arranged by size: 1645 1524 788 787 597 554 393 268 EcoO109I rG'GnC_Cy Cuts at: 2029 2030 2299 2029 Size: 1 269 6286 Fragments arranged by size: 6286 269 1 EcoRI G'AATT_C Cuts at: 3865 4243 3865 Size: 378 6178 FspI TGC'GCA Cuts at: 5062 5062 Size: 6556 HaeII r_GCGC'y Cuts at: 1678 5703 1678 Size: 4025 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2467 2499 5197 5229 5271 Size: 32 135 32 895 32 2698 32 42 Cuts at: 5271 5303 1373 Size: 32 2626 Fragments arranged by size: 2698 2626 895 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3198 3900 4146 669 Size: 988 281 1260 702 246 3079 Fragments arranged by size: 3079 1260 988 702 281 246 HindIII A'AGCT_T Cuts at: 748 2365 748 Size: 1617 4939 HpaI GTT'AAC Cuts at: 4146 4146 Size: 6556 KpnI G_GTAC'C Cuts at: 2012 3881 2012 Size: 1869 4687 MfeI C'AATT_G Cuts at: 2637 3432 4155 2637 Size: 795 723 5038 Fragments arranged by size: 5038 795 723 MluI A'CGCG_T Cuts at: 6432 6432 Size: 6556 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2908 2953 5670 5854 1305 Size: 1603 45 2717 184 2007 Fragments arranged by size: 2717 2007 1603 184 45 MscI TGG'CCA Cuts at: 10 64 1489 1832 2883 10 Size: 54 1425 343 1051 3683 Fragments arranged by size: 3683 1425 1051 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3110 3592 4514 4873 5032 518 Size: 648 1944 482 922 359 159 2042 Fragments arranged by size: 2042 1944 922 648 482 359 159 MspA1I CmG'CkG Cuts at: 1606 2079 3972 4649 5797 6042 1606 Size: 473 1893 677 1148 245 2120 Fragments arranged by size: 2120 1893 1148 677 473 245 NaeI GCC'GGC Cuts at: 2087 2087 Size: 6556 NcoI C'CATG_G Cuts at: 513 1065 2004 2584 3084 3501 3587 513 Size: 552 939 580 500 417 86 3482 Fragments arranged by size: 3482 939 580 552 500 417 86 NdeI CA'TA_TG Cuts at: 387 387 Size: 6556 NgoMIV G'CCGG_C Cuts at: 2085 2085 Size: 6556 NheI G'CTAG_C Cuts at: 1052 2702 2951 3694 1052 Size: 1650 249 743 3914 Fragments arranged by size: 3914 1650 743 249 NotI GC'GGCC_GC Cuts at: 4003 4003 Size: 6556 NsiI A_TGCA'T Cuts at: 2908 2908 Size: 6556 NspI r_CATG'y Cuts at: 1132 1229 2119 2602 2830 3914 5459 1132 Size: 97 890 483 228 1084 1545 2229 Fragments arranged by size: 2229 1545 1084 890 483 228 97 PasI CC'CwG_GG Cuts at: 1348 2097 3362 1348 Size: 749 1265 4542 Fragments arranged by size: 4542 1265 749 PciI A'CATG_T Cuts at: 2598 5455 2598 Size: 2857 3699 PflMI CCAn_nnn'nTGG Cuts at: 1108 3160 1108 Size: 2052 4504 PmeI GTTT'AAAC Cuts at: 3860 3860 Size: 6556 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4625 4657 6138 6170 4625 Size: 32 1481 32 5011 Fragments arranged by size: 5011 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2299 2299 Size: 6556 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6556 PsiI TTA'TAA Cuts at: 4126 4126 Size: 6556 PspOMI G'GGCC_C Cuts at: 2029 2029 Size: 6556 PstI C_TGCA'G Cuts at: 830 3134 3908 830 Size: 2304 774 3478 Fragments arranged by size: 3478 2304 774 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6556 SacI G_AGCT'C Cuts at: 2258 2258 Size: 6556 SalI G'TCGA_C Cuts at: 1936 3898 1936 Size: 1962 4594 SapI GCTCTTCn'nnn_ Cuts at: 2202 2202 Size: 6556 SbfI CC_TGCA'GG Cuts at: 3908 3908 Size: 6556 ScaI AGT'ACT Cuts at: 4804 6516 4804 Size: 1712 4844 SfcI C'TryA_G Cuts at: 826 1045 3130 3904 5039 5720 5911 826 Size: 219 2085 774 1135 681 191 1471 Fragments arranged by size: 2085 1471 1135 774 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6556 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6556 SmaI CCC'GGG Cuts at: 1872 3883 1872 Size: 2011 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4660 5561 5823 820 Size: 197 926 2717 901 262 1553 Fragments arranged by size: 2717 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4347 493 Size: 3854 2702 SpeI A'CTAG_T Cuts at: 152 2857 152 Size: 2705 3851 SphI G_CATG'C Cuts at: 1132 3914 1132 Size: 2782 3774 SspI AAT'ATT Cuts at: 5 52 4480 5 Size: 47 4428 2081 Fragments arranged by size: 4428 2081 47 StuI AGG'CCT Cuts at: 1517 2499 1517 Size: 982 5574 StyI C'CwwG_G Cuts at: 513 1065 2004 2584 2619 3084 3159 3501 3587 Size: 552 939 580 35 465 75 342 86 Cuts at: 3587 3995 513 Size: 408 3074 Fragments arranged by size: 3074 939 580 552 465 408 342 86 75 35 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3137 3580 4583 4600 4753 4938 5383 Size: 1255 1557 443 1003 17 153 185 445 Cuts at: 5383 325 Size: 1498 Fragments arranged by size: 1557 1498 1255 1003 445 443 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2595 2790 2869 Size: 274 80 33 51 1443 618 195 79 Cuts at: 2869 4802 6514 96 Size: 1933 1712 138 Fragments arranged by size: 1933 1712 1443 618 274 195 138 80 79 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6516 6548 6516 Size: 32 6524 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6556 XbaI T'CTAG_A Cuts at: 3892 3892 Size: 6556 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6556 XmaI C'CCGG_G Cuts at: 1870 3881 1870 Size: 2011 4545 XmnI GAAnn'nnTTC Cuts at: 4685 4685 Size: 6556 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3370 4364 276 Size: 53 83 186 936 1836 994 2468 Fragments arranged by size: 2468 1836 994 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NarI NruI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI