################################## # NO CUT ENZYMES ( 134 enzymes) # ################################## AatII Acc65I AccII AccIII AcyI AflIII AgeI AhdI AlwNI AocI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BanI BbeI BbrPI BbvCI BcgI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraIII EagI Eam1105I EclXI Eco52I Eco81I EcoO65I EcoT22I FseI FspI HgaI Hin1I HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AccI 460 GTCTAC GT^[AC][GT]AC AclI 1423 AACGTT AA^CGTT AfaI 1271 GTAC GT^AC Alw44I 45 GTGCAC G^TGCAC Aor51HI 276 AGCGCT AGC^GCT ApaLI 45 GTGCAC G^TGCAC AspHI 45 GTGCAC G[AT]GC[AT]^C AvaII 99 GGACC G^G[AT]CC BalI 759 TGGCCA TGG^CCA BamHI 1122 GGATCC G^GATCC BanII 232 GAGCCC G[GA]GC[CT]^C BbsI 4 GAAGAC GAAGAC(2/6) BciVI 357 GTATCC GTATCC(6/5) BclI 1218 TGATCA T^GATCA BcnI 1094 CCGGG CC^[GC]GG BglII 942 AGATCT A^GATCT BpuAI 4 GAAGAC GAAGAC(2/6) BsiHKAI 45 GTGCAC G[AT]GC[AT]^C BsmAI 210 GTCTC GTCTC(1/5) BstXI 80 CCAGGTCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 277 GCGC GCG^C DraI 18 TTTAAA TTT^AAA DraII 98 AGGACCT [GA]G^G[ACGT]CC[CT] DrdI 201 GACAGGAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 1130 CCATGG C^C[GA][CT]GG EaeI 759 TGGCCA [CT]^GGCC[GA] Eco47III 276 AGCGCT AGC^GCT EcoNI 1781 CCTTCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 98 AGGACCT [GA]G^G[ACGT]CC[CT] EcoRI 75 GAATTC G^AATTC EcoRV 1791 GATATC GAT^ATC FauI 1014 CCCGC CCCGC(4/6) FbaI 1218 TGATCA T^GATCA HaeII 276 AGCGCT [GA]GCGC^[CT] HhaI 277 GCGC GCG^C HinP1I 277 GCGC G^CGC HincII 1354 GTTGAC GT[CT]^[GA]AC HindII 1354 GTTGAC GT[CT]^[GA]AC MluNI 759 TGGCCA TGG^CCA MscI 759 TGGCCA TGG^CCA MslI 1228 CATATAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NciI 1094 CCGGG CC^[GC]GG NcoI 1130 CCATGG C^CATGG NlaIV 1122 GGATCC GG[ACGT]^[ACGT]CC NspI 1775 ACATGC [GA]CATG^[CT] PflMI 1824 CCAAGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 98 AGGACCT [GA]G^G[AT]CC[CT] Psp1406I 1423 AACGTT AA^CGTT RsaI 1271 GTAC GT^AC SspI 1645 AATATT AAT^ATT SwaI 17 ATTTAAAT ATTT^AAAT Van91I 1824 CCAAGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 503 CCACTTACGATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AciI 1015 CCGC CCGC(-3/-1) 1152 CCGC AcsI 75 GAATTC [GA]^AATT[CT] 714 AAATTT AflII 1266 CTTAAG C^TTAAG 1519 CTTAAG ApoI 75 GAATTC [GA]^AATT[CT] 714 AAATTT BfrI 1266 CTTAAG C^TTAAG 1519 CTTAAG BmyI 45 GTGCAC G[AGT]GC[ACT]^C 232 GAGCCC BseRI 1210 GAGGAG GAGGAG(10/8) 1346 GAGGAG Bsp1286I 45 GTGCAC G[AGT]GC[ACT]^C 232 GAGCCC BstAPI 1309 GCAAGTAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1770 GCACAACATGC BstYI 942 AGATCT [GA]^GATC[CT] 1122 GGATCC Cfr13I 99 GGACC G^G[ACGT]CC 1441 GGCCC HapII 996 CCGG C^CGG 1094 CCGG HpaII 996 CCGG C^CGG 1094 CCGG HphI 133 GGTGA GGTGA(8/7) 1091 TCACC MflI 942 AGATCT [GA]^GATC[CT] 1122 GGATCC MspA1I 352 CAGCTG C[AC]G^C[GT]G 1304 CAGCTG MspI 996 CCGG C^CGG 1094 CCGG MwoI 1309 GCAAGTAGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 1770 GCACAACATGC PvuII 352 CAGCTG CAG^CTG 1304 CAGCTG Sau96I 99 GGACC G^G[ACGT]CC 1441 GGCCC SmlI 1266 CTTAAG C^T[CT][GA]AG 1519 CTTAAG TaqI 817 TCGA T^CGA 908 TCGA Tsp45I 1028 GTGAC ^GT[GC]AC 1799 GTCAC TthHB8I 817 TCGA T^CGA 908 TCGA XhoII 942 AGATCT [GA]^GATC[CT] 1122 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 46 enzymes) # ######################################### AluI AlwI BbvI BfaI BpmI BsaJI BsiYI BslI BsmFI BsrI BssKI BstF5I BstNI Cac8I DdeI DpnII EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MvaI NdeII NlaIII PleI Sau3AI ScrFI SfaNI SfcI StyI TfiI Tru9I TseI Tsp509I TspRI