(Circular) (Six-base) MAPSORT of: pFN21AE5720.seq Check: 6824 from: 1 to: 6553 pFN21AE5720 6553 bp With 182 enzymes: SgfI * December 8, 2014 13:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4363 278 Size: 53 83 186 936 2827 2468 Fragments arranged by size: 2827 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2466 3896 1937 Size: 529 1430 4594 Fragments arranged by size: 4594 1430 529 Acc65I G'GTAC_C Cuts at: 3874 3874 Size: 6553 AclI AA'CG_TT Cuts at: 1563 3429 4680 5053 1563 Size: 1866 1251 373 3063 Fragments arranged by size: 3063 1866 1251 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4616 6000 6268 1632 Size: 2984 1384 268 1917 Fragments arranged by size: 2984 1917 1384 268 AfeI AGC'GCT Cuts at: 2283 2283 Size: 6553 AflII C'TTAA_G Cuts at: 820 1017 3271 3524 820 Size: 197 2254 253 3849 Fragments arranged by size: 3849 2254 253 197 AflIII A'CryG_T Cuts at: 1203 5452 6429 1203 Size: 4249 977 1327 Fragments arranged by size: 4249 1327 977 AgeI A'CCGG_T Cuts at: 5384 5384 Size: 6553 AhdI GACnn_n'nnGTC Cuts at: 5282 5282 Size: 6553 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6553 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6553 AlwNI CAG_nnn'CTG Cuts at: 4296 5868 4296 Size: 1572 4981 ApaLI G'TGCA_C Cuts at: 2050 4610 5766 2050 Size: 2560 1156 2837 Fragments arranged by size: 2837 2560 1156 ApoI r'AATT_y Cuts at: 1493 2080 2719 3862 4088 4240 6349 1493 Size: 587 639 1143 226 152 2109 1697 Fragments arranged by size: 2109 1697 1143 639 587 226 152 AseI AT'TA_AT Cuts at: 160 1032 5107 160 Size: 872 4075 1606 Fragments arranged by size: 4075 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6553 AvaI C'yCGr_G Cuts at: 1870 1943 3878 1870 Size: 73 1935 4545 Fragments arranged by size: 4545 1935 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2345 2378 3259 3292 2345 Size: 33 881 33 5606 Fragments arranged by size: 5606 881 33 33 BamHI G'GATC_C Cuts at: 3127 3883 4250 3127 Size: 756 367 5430 Fragments arranged by size: 5430 756 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3874 5329 618 Size: 325 217 627 2087 1455 1842 Fragments arranged by size: 2087 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2241 3872 1391 Size: 850 1631 4072 Fragments arranged by size: 4072 1631 850 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2016 928 Size: 971 117 5465 Fragments arranged by size: 5465 971 117 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4744 4778 1903 Size: 34 2807 34 3678 Fragments arranged by size: 3678 2807 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2373 4445 5655 2373 Size: 2072 1210 3271 Fragments arranged by size: 3271 2072 1210 BclI T'GATC_A Cuts at: 1548 3223 1548 Size: 1675 4878 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5164 136 Size: 107 122 71 631 605 3492 1525 Fragments arranged by size: 3492 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2947 6548 2947 Size: 3601 2952 BlpI GC'TnA_GC Cuts at: 3970 3970 Size: 6553 Bme1580I G_kGCm'C Cuts at: 1790 2054 4614 5770 1790 Size: 264 2560 1156 2573 Fragments arranged by size: 2573 2560 1156 264 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6553 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2307 4284 5242 Size: 416 53 83 399 24 890 1977 958 Cuts at: 5242 442 Size: 1753 Fragments arranged by size: 1977 1753 958 890 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6553 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2130 2292 3059 3811 5213 1239 Size: 891 162 767 752 1402 2579 Fragments arranged by size: 2579 1402 891 767 752 162 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4678 5543 5841 4678 Size: 865 298 5390 Fragments arranged by size: 5390 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5216 882 Size: 617 3717 2219 Fragments arranged by size: 3717 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4344 493 Size: 711 3140 2702 Fragments arranged by size: 3140 2702 711 BsaBI GATnn'nnATC Cuts at: 6547 6547 Size: 6553 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4360 4742 5377 275 Size: 53 83 186 936 2827 382 635 1451 Fragments arranged by size: 2827 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4986 5384 5658 5805 6216 4986 Size: 398 274 147 411 5323 Fragments arranged by size: 5323 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3032 3062 1178 Size: 30 165 30 1629 30 4669 Fragments arranged by size: 4669 1629 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3230 3366 1186 Size: 2044 136 4373 Fragments arranged by size: 4373 2044 136 BseYI C'CCAG_C Cuts at: 3595 5756 6252 3595 Size: 2161 496 3896 Fragments arranged by size: 3896 2161 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5980 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4003 4764 5792 1540 Size: 275 186 2002 761 1028 2301 Fragments arranged by size: 2301 2002 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2054 4614 4699 5770 2054 Size: 2560 85 1071 2837 Fragments arranged by size: 2837 2560 1071 85 BsmI GAATG_Cn' Cuts at: 4064 4157 4064 Size: 93 6460 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2054 2241 3872 4614 4699 5770 1391 Size: 399 264 187 1631 742 85 1071 2174 Fragments arranged by size: 2174 1631 1071 742 399 264 187 85 BspEI T'CCGG_A Cuts at: 6216 6216 Size: 6553 BspHI T'CATG_A Cuts at: 4442 6172 4442 Size: 1730 4823 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3908 844 Size: 3064 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4440 1394 Size: 429 2617 3507 Fragments arranged by size: 3507 2617 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5048 5222 65 Size: 1183 3800 174 1396 Fragments arranged by size: 3800 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5197 5384 1700 Size: 110 3387 187 2869 Fragments arranged by size: 3387 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6553 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6553 BssSI C'ACGA_G Cuts at: 4613 5625 4613 Size: 1012 5541 BstAPI GCAn_nnn'nTGC Cuts at: 794 3320 3781 794 Size: 2526 461 3566 Fragments arranged by size: 3566 2526 461 BstBI TT'CG_AA Cuts at: 5357 5357 Size: 6553 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6553 BstXI CCAn_nnnn'nTGG Cuts at: 2092 2092 Size: 6553 BstYI r'GATC_y Cuts at: 1972 2947 3127 3883 4250 4635 4652 6104 6190 Size: 975 180 756 367 385 17 1452 86 Cuts at: 6190 6202 6548 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 975 756 385 367 346 180 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3135 513 Size: 552 939 1131 3931 Fragments arranged by size: 3931 1131 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5814 Fragments arranged by size: 5814 683 56 BtsI GCAGTG_nn' Cuts at: 2257 3089 4069 4863 4883 2257 Size: 832 980 794 20 3927 Fragments arranged by size: 3927 980 832 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6553 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6518 DraI TTT'AAA Cuts at: 2025 3857 2025 Size: 1832 4721 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2212 5560 809 Size: 507 896 3348 1802 Fragments arranged by size: 3348 1802 896 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2764 4000 4889 Size: 54 1425 50 275 18 934 1236 889 Cuts at: 4889 6518 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1236 934 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4000 1537 Size: 275 2188 4090 Fragments arranged by size: 4090 2188 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2223 2854 2989 3388 4483 1353 Size: 870 631 135 399 1095 3423 Fragments arranged by size: 3423 1095 870 631 399 135 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5140 5514 5660 387 Size: 1064 138 3551 374 146 1280 Fragments arranged by size: 3551 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2130 2292 3059 3811 4616 5213 6000 Size: 393 498 162 767 752 805 597 787 Cuts at: 6000 6268 1239 Size: 268 1524 Fragments arranged by size: 1524 805 787 767 752 597 498 393 268 162 EcoNI CCTnn'n_nnAGG Cuts at: 3790 3790 Size: 6553 EcoO109I rG'GnC_Cy Cuts at: 2104 2104 Size: 6553 EcoRI G'AATT_C Cuts at: 2080 3862 4240 2080 Size: 1782 378 4393 Fragments arranged by size: 4393 1782 378 EcoRV GAT'ATC Cuts at: 3798 3798 Size: 6553 FspI TGC'GCA Cuts at: 5059 5059 Size: 6553 HaeII r_GCGC'y Cuts at: 1678 2285 5700 1678 Size: 607 3415 2531 Fragments arranged by size: 3415 2531 607 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2732 2764 3163 3195 3233 Size: 32 135 32 1160 32 399 32 38 Cuts at: 3233 3265 3478 3510 3787 3819 5194 5226 5268 Size: 32 213 32 277 32 1375 32 42 Cuts at: 5268 5300 1373 Size: 32 2626 Fragments arranged by size: 2626 1375 1160 399 277 213 135 42 38 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3361 3897 4143 669 Size: 988 281 1423 536 246 3079 Fragments arranged by size: 3079 1423 988 536 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6553 HpaI GTT'AAC Cuts at: 4143 4143 Size: 6553 KpnI G_GTAC'C Cuts at: 3878 3878 Size: 6553 MfeI C'AATT_G Cuts at: 4152 4152 Size: 6553 MluI A'CGCG_T Cuts at: 6429 6429 Size: 6553 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2110 3412 5667 5851 1305 Size: 805 1302 2255 184 2007 Fragments arranged by size: 2255 2007 1302 805 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2766 10 Size: 54 1425 343 934 3797 Fragments arranged by size: 3797 1425 934 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3237 4511 4870 5029 518 Size: 648 2071 1274 359 159 2042 Fragments arranged by size: 2071 2042 1274 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2359 3311 3969 4646 5794 6039 1606 Size: 753 952 658 677 1148 245 2120 Fragments arranged by size: 2120 1148 952 753 677 658 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3135 513 Size: 552 939 1131 3931 Fragments arranged by size: 3931 1131 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6553 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6553 NotI GC'GGCC_GC Cuts at: 4000 4000 Size: 6553 NspI r_CATG'y Cuts at: 1132 1229 3784 3911 5456 1132 Size: 97 2555 127 1545 2229 Fragments arranged by size: 2555 2229 1545 127 97 PasI CC'CwG_GG Cuts at: 1348 2606 1348 Size: 1258 5295 PciI A'CATG_T Cuts at: 5452 5452 Size: 6553 PflMI CCAn_nnn'nTGG Cuts at: 1108 3835 1108 Size: 2727 3826 PmeI GTTT'AAAC Cuts at: 3857 3857 Size: 6553 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4622 4654 6135 6167 4622 Size: 32 1481 32 5008 Fragments arranged by size: 5008 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2104 2104 Size: 6553 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6553 PsiI TTA'TAA Cuts at: 4123 4123 Size: 6553 PstI C_TGCA'G Cuts at: 830 3905 830 Size: 3075 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6553 PvuII CAG'CTG Cuts at: 2359 3311 2359 Size: 952 5601 SalI G'TCGA_C Cuts at: 1936 3895 1936 Size: 1959 4594 SbfI CC_TGCA'GG Cuts at: 3905 3905 Size: 6553 ScaI AGT'ACT Cuts at: 4801 6513 4801 Size: 1712 4841 SfcI C'TryA_G Cuts at: 826 1045 2323 2497 2965 3280 3470 3619 3901 Size: 219 1278 174 468 315 190 149 282 Cuts at: 3901 5036 5717 5908 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1278 1135 681 468 315 282 219 191 190 174 149 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6553 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6553 SmaI CCC'GGG Cuts at: 1872 3880 1872 Size: 2008 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3271 3524 4657 5558 5820 820 Size: 197 926 1328 253 1133 901 262 1553 Fragments arranged by size: 1553 1328 1133 926 901 262 253 197 SnaBI TAC'GTA Cuts at: 493 4344 493 Size: 3851 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6553 SphI G_CATG'C Cuts at: 1132 3911 1132 Size: 2779 3774 SspI AAT'ATT Cuts at: 5 52 3652 4477 5 Size: 47 3600 825 2081 Fragments arranged by size: 3600 2081 825 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6553 StyI C'CwwG_G Cuts at: 513 1065 2004 3135 3148 3442 3992 513 Size: 552 939 1131 13 294 550 3074 Fragments arranged by size: 3074 1131 939 552 550 294 13 SwaI ATTT'AAAT Cuts at: 2025 2025 Size: 6553 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2510 2953 4580 4597 4750 4935 5380 Size: 1255 930 443 1627 17 153 185 445 Cuts at: 5380 325 Size: 1498 Fragments arranged by size: 1627 1498 1255 930 445 443 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4799 6511 96 Size: 274 80 33 51 1443 2822 1712 138 Fragments arranged by size: 2822 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6513 6545 6513 Size: 32 6521 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6553 XbaI T'CTAG_A Cuts at: 3889 3889 Size: 6553 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2515 2515 Size: 6553 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6553 XmaI C'CCGG_G Cuts at: 1870 3878 1870 Size: 2008 4545 XmnI GAAnn'nnTTC Cuts at: 4682 4682 Size: 6553 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4361 276 Size: 53 83 186 936 2827 2468 Fragments arranged by size: 2827 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI