(Linear) (Six-base) MAPSORT of: KIEE5720.seq Check: 3301 from: 1 to: 1848 KIEE5720.orf With 182 enzymes: SgfI * December 5, 2014 13:08 .. AccI GT'mk_AC Cuts at: 0 461 1848 Size: 461 1387 AclI AA'CG_TT Cuts at: 0 1424 1848 Size: 1424 424 AfeI AGC'GCT Cuts at: 0 278 1848 Size: 278 1570 AflII C'TTAA_G Cuts at: 0 1266 1519 1848 Size: 1266 253 329 Fragments arranged by size: 1266 329 253 ApaLI G'TGCA_C Cuts at: 0 45 1848 Size: 45 1803 ApoI r'AATT_y Cuts at: 0 75 714 1848 Size: 75 639 1134 Fragments arranged by size: 1134 639 75 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 340 373 1254 1287 1848 Size: 340 33 881 33 561 Fragments arranged by size: 881 561 340 33 33 BamHI G'GATC_C Cuts at: 0 1122 1848 Size: 1122 726 BanII G_rGCy'C Cuts at: 0 236 1848 Size: 236 1612 BbsI GAAGACnn'nnnn_ Cuts at: 0 11 1848 Size: 11 1837 BciVI GTATCCnnnnn_n' Cuts at: 0 368 1848 Size: 368 1480 BclI T'GATC_A Cuts at: 0 1218 1848 Size: 1218 630 BglII A'GATC_T Cuts at: 0 942 1848 Size: 942 906 Bme1580I G_kGCm'C Cuts at: 0 49 1848 Size: 49 1799 BmrI ACTGGGnnnn_n' Cuts at: 0 302 1848 Size: 302 1546 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 125 287 1054 1806 1848 Size: 125 162 767 752 42 Fragments arranged by size: 767 752 162 125 42 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1027 1057 1848 Size: 1027 30 791 Fragments arranged by size: 1027 791 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1225 1361 1848 Size: 1225 136 487 Fragments arranged by size: 1225 487 136 BseYI C'CCAG_C Cuts at: 0 1590 1848 Size: 1590 258 BsiHKAI G_wGCw'C Cuts at: 0 49 1848 Size: 49 1799 Bsp1286I G_dGCh'C Cuts at: 0 49 236 1848 Size: 49 187 1612 Fragments arranged by size: 1612 187 49 BstAPI GCAn_nnn'nTGC Cuts at: 0 1315 1776 1848 Size: 1315 461 72 Fragments arranged by size: 1315 461 72 BstXI CCAn_nnnn'nTGG Cuts at: 0 87 1848 Size: 87 1761 BstYI r'GATC_y Cuts at: 0 942 1122 1848 Size: 942 180 726 Fragments arranged by size: 942 726 180 BtgI C'CryG_G Cuts at: 0 1130 1848 Size: 1130 718 BtsI GCAGTG_nn' Cuts at: 0 252 1084 1848 Size: 252 832 764 Fragments arranged by size: 832 764 252 DraI TTT'AAA Cuts at: 0 20 1848 Size: 20 1828 DrdI GACnn_nn'nnGTC Cuts at: 0 207 1848 Size: 207 1641 EaeI y'GGCC_r Cuts at: 0 759 1848 Size: 759 1089 EarI CTCTTCn'nnn_ Cuts at: 0 218 849 984 1383 1848 Size: 218 631 135 399 465 Fragments arranged by size: 631 465 399 218 135 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 125 287 1054 1806 1848 Size: 125 162 767 752 42 Fragments arranged by size: 767 752 162 125 42 EcoNI CCTnn'n_nnAGG Cuts at: 0 1785 1848 Size: 1785 63 EcoO109I rG'GnC_Cy Cuts at: 0 99 1848 Size: 99 1749 EcoRI G'AATT_C Cuts at: 0 75 1848 Size: 75 1773 EcoRV GAT'ATC Cuts at: 0 1793 1848 Size: 1793 55 HaeII r_GCGC'y Cuts at: 0 280 1848 Size: 280 1568 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 727 759 1158 1190 1228 1260 1473 1505 Size: 727 32 399 32 38 32 213 32 Cuts at: 1505 1782 1814 1848 Size: 277 32 34 Fragments arranged by size: 727 399 277 213 38 34 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 1356 1848 Size: 1356 492 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 105 1407 1848 Size: 105 1302 441 Fragments arranged by size: 1302 441 105 MscI TGG'CCA Cuts at: 0 761 1848 Size: 761 1087 MslI CAynn'nnrTG Cuts at: 0 1232 1848 Size: 1232 616 MspA1I CmG'CkG Cuts at: 0 354 1306 1848 Size: 354 952 542 Fragments arranged by size: 952 542 354 NcoI C'CATG_G Cuts at: 0 1130 1848 Size: 1130 718 NspI r_CATG'y Cuts at: 0 1779 1848 Size: 1779 69 PasI CC'CwG_GG Cuts at: 0 601 1848 Size: 601 1247 PflMI CCAn_nnn'nTGG Cuts at: 0 1830 1848 Size: 1830 18 PpuMI rG'GwC_Cy Cuts at: 0 99 1848 Size: 99 1749 PvuII CAG'CTG Cuts at: 0 354 1306 1848 Size: 354 952 542 Fragments arranged by size: 952 542 354 SfcI C'TryA_G Cuts at: 0 318 492 960 1275 1465 1614 1848 Size: 318 174 468 315 190 149 234 Fragments arranged by size: 468 318 315 234 190 174 149 SmlI C'TyrA_G Cuts at: 0 1266 1519 1848 Size: 1266 253 329 Fragments arranged by size: 1266 329 253 SspI AAT'ATT Cuts at: 0 1647 1848 Size: 1647 201 StyI C'CwwG_G Cuts at: 0 1130 1143 1437 1848 Size: 1130 13 294 411 Fragments arranged by size: 1130 411 294 13 SwaI ATTT'AAAT Cuts at: 0 20 1848 Size: 20 1828 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 505 948 1848 Size: 505 443 900 Fragments arranged by size: 900 505 443 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 510 1848 Size: 510 1338 Enzymes that do cut: AccI AclI AfeI AflII ApaLI ApoI BaeI BamHI BanII BbsI BciVI BclI BglII Bme1580I BmrI BpmI BsaXI BseRI BseYI BsiHKAI Bsp1286I BstAPI BstXI BstYI BtgI BtsI DraI DrdI EaeI EarI Eco57MI EcoNI EcoO109I EcoRI EcoRV HaeII Hin4I HincII MmeI MscI MslI MspA1I NcoI NspI PasI PflMI PpuMI PvuII SfcI SmlI SspI StyI SwaI TaqII XcmI Enzymes that do not cut: AarI AatII Acc65I AcuI AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI AscI AseI AsiSI AvaI AvrII BanI BbeI BbvCI BcgI BfrBI BglI BlpI BmgBI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraIII EagI EciI EcoICRI FalI FseI FspI FspAI HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI