################################## # NO CUT ENZYMES ( 156 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AlwI AocI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BanII BbeI BbrPI BciVI BcnI BfrI BglI BlnI BpmI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraII DraIII DsaI EaeI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HapII HgaI Hin1I HindIII HinfI HpaI HpaII KasI KpnI KspI MaeII MamI MfeI MluI MluNI MroI MscI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SbfI ScaI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI TfiI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AccI 820 GTCGAC GT^[AC][GT]AC AcsI 592 AAATTT [GA]^AATT[CT] AflIII 239 ACATGT A^C[GA][CT]GT Aor51HI 189 AGCGCT AGC^GCT ApoI 592 AAATTT [GA]^AATT[CT] Asp700I 966 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 31 GAGCAC G[AT]GC[AT]^C BanI 480 GGTGCC G^G[CT][GA]CC BbvCI 267 CCTCAGC CCTCAGC(-5/-2) BcgI 490 GCATACACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 644 TGATCA T^GATCA BfaI 148 CTAG C^TAG BglII 662 AGATCT A^GATCT BsiHKAI 31 GAGCAC G[AT]GC[AT]^C BsmAI 526 GAGAC GTCTC(1/5) BsmI 43 GCATTC GAATGC(1/-1) BspLU11I 239 ACATGT A^CATGT BsrDI 765 CATTGC GCAATG(2/0) BstYI 662 AGATCT [GA]^GATC[CT] CfoI 190 GCGC GCG^C Cfr13I 228 GGCCC G^G[ACGT]CC DrdI 811 GACACGATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 189 AGCGCT AGC^GCT EcoT14I 18 CCAAGG C^C[AT][AT]GG FauI 484 CCCGC CCCGC(4/6) FbaI 644 TGATCA T^GATCA HaeII 189 AGCGCT [GA]GCGC^[CT] HhaI 190 GCGC GCG^C HinP1I 190 GCGC G^CGC HincII 820 GTCGAC GT[CT]^[GA]AC HindII 820 GTCGAC GT[CT]^[GA]AC MaeI 148 CTAG C^TAG MaeIII 410 GTGAC ^GT[ACGT]AC MflI 662 AGATCT [GA]^GATC[CT] MseI 607 TTAA T^TAA MspA1I 577 CAGCTG C[AC]G^C[GT]G NlaIV 480 GGTGCC GG[ACGT]^[ACGT]CC PflMI 540 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 577 CAGCTG CAG^CTG SalI 820 GTCGAC G^TCGAC SapI 28 GAAGAGC GCTCTTC(1/4) Sau96I 228 GGCCC G^G[ACGT]CC SexAI 53 ACCAGGT A^CC[AT]GGT SphI 131 GCATGC GCATG^C StyI 18 CCAAGG C^C[AT][AT]GG TaqI 821 TCGA T^CGA Tru9I 607 TTAA T^TAA Tsp45I 410 GTGAC ^GT[GC]AC Tsp509I 593 AATT ^AATT TthHB8I 821 TCGA T^CGA Van91I 540 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 662 AGATCT [GA]^GATC[CT] XmnI 966 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AlwNI 541 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG 577 CAGCTGCTG BbsI 10 GTCTTC GAAGAC(2/6) 970 GTCTTC BlpI 534 GCTCAGC GC^T[ACGT]AGC 688 GCTCAGC BmyI 31 GAGCAC G[AGT]GC[ACT]^C 481 GTGCCC Bpu1102I 534 GCTCAGC GC^T[ACGT]AGC 688 GCTCAGC BpuAI 10 GTCTTC GAAGAC(2/6) 970 GTCTTC BsgI 325 CTGCAC GTGCAG(16/14) 122 GTGCAG BsmFI 308 GGGAC GGGAC(10/14) 628 GTCCC Bsp1286I 31 GAGCAC G[AGT]GC[ACT]^C 481 GTGCCC BstXI 54 CCAGGTGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 752 CCAAGACCCTGG CelII 534 GCTCAGC GC^T[ACGT]AGC 688 GCTCAGC DpnII 645 GATC ^GATC 663 GATC HaeIII 117 GGCC GG^CC 228 GGCC MboI 645 GATC ^GATC 663 GATC MslI 513 CACAGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 765 CATTGCTGTG NdeII 645 GATC ^GATC 663 GATC NspI 131 GCATGC [GA]CATG^[CT] 239 ACATGT Sau3AI 645 GATC ^GATC 663 GATC SfaNI 555 GATGC GCATC(5/9) 95 GCATC XcmI 275 CCATTCGCTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 806 CCATAGACACGATGG ######################################### # 3 OR MORE CUT ENZYMES ( 29 enzymes) # ######################################### AciI AfaI AluI BbvI BsaJI BsiYI BslI BsrI BssKI BstF5I BstNI Cac8I DdeI EarI EcoRII Fnu4HI FokI HphI ItaI Ksp632I MboII MnlI MvaI MwoI NlaIII RsaI ScrFI TseI TspRI