################################## # NO CUT ENZYMES ( 211 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssKI BssSI Bst1107I BstBI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I Cfr13I ClaI CpoI DpnII DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoRV EcoT14I EcoT22I FauI FbaI FokI FseI FspI HaeII HapII HgaI Hin1I HincII HindII HindIII HpaI HpaII HphI KasI KpnI KspI MamI MboI MfeI MflI MluI MluNI MroI MscI MspA1I MspI MunI MvaI MvnI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NlaIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI Sau3AI Sau96I SbfI ScaI ScrFI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 30 enzymes) # ################################### AclI 346 AACGTT AA^CGTT BbsI 139 GAAGAC GAAGAC(2/6) BbvI 334 GCAGC GCAGC(8/12) BcgI 365 GCAAGAAGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BpuAI 139 GAAGAC GAAGAC(2/6) BseRI 97 GAGGAG GAGGAG(10/8) BsiYI 37 CCGTTGCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 37 CCGTTGCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmBI 423 GAGACG CGTCTC(1/5) BsrI 68 CCAGT ACTGG(1/-1) BstAPI 261 GCATGACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CfoI 7 GCGC GCG^C EaeI 356 CGGCCA [CT]^GGCC[GA] EarI 109 GAAGAG CTCTTC(1/4) Fnu4HI 334 GCAGC GC^[ACGT]GC HaeIII 357 GGCC GG^CC HhaI 7 GCGC GCG^C HinP1I 7 GCGC G^CGC HinfI 309 GAGTC G^A[ACGT]TC ItaI 334 GCAGC GC^[ACGT]GC Ksp632I 109 GAAGAG CTCTTC(1/4) MaeII 347 ACGT A^CGT MslI 405 CATAAGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG PleI 309 GAGTC GAGTC(4/5) Psp1406I 346 AACGTT AA^CGTT PstI 332 CTGCAG CTGCA^G SfcI 332 CTGCAG C^T[GA][CT]AG SphI 86 GCATGC GCATG^C TseI 334 GCAGC G^C[AT]GC Tsp45I 311 GTCAC ^GT[GC]AC ################################### # TWO CUT ENZYMES ( 9 enzymes) # ################################### DdeI 95 CTGAG C^T[ACGT]AG 307 CTGAG MaeIII 311 GTCAC ^GT[ACGT]AC 386 GTTAC MseI 115 TTAA T^TAA 235 TTAA MwoI 261 GCATGACATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 328 GCAACTGCAGC NspI 86 GCATGC [GA]CATG^[CT] 266 ACATGC SfaNI 58 GATGC GCATC(5/9) 186 GCATC Tru9I 115 TTAA T^TAA 235 TTAA Tsp509I 147 AATT ^AATT 233 AATT TspRI 69 CAGTG CA[GC]TG[ACGT][ACGT]^ 279 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 8 enzymes) # ######################################### AluI BfaI BsmAI Cac8I MaeI MboII MnlI NlaIII