Restriction map for
pFN21AE5163
(Circular) (Six-base) MAPSORT of: pFN21AE5163.seq Check: 2400 from: 1 to: 5137
pFN21AE5163 5137 bp
With 182 enzymes: SgfI *
January 30, 2014 17:13 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2947 278
Size: 53 83 186 936 1411 2468
Fragments arranged by size:
2468 1411 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2480 1937
Size: 543 4594
Acc65I G'GTAC_C
Cuts at: 2458 2458
Size: 5137
AclI AA'CG_TT
Cuts at: 1563 2352 3264 3637 1563
Size: 789 912 373 3063
Fragments arranged by size:
3063 912 789 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2236 3200 4584 4852 1632
Size: 604 964 1384 268 1917
Fragments arranged by size:
1917 1384 964 604 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4940
AflIII A'CryG_T
Cuts at: 1203 4036 5013 1203
Size: 2833 977 1327
Fragments arranged by size:
2833 1327 977
AgeI A'CCGG_T
Cuts at: 3968 3968
Size: 5137
AhdI GACnn_n'nnGTC
Cuts at: 3866 3866
Size: 5137
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5137
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5137
AlwNI CAG_nnn'CTG
Cuts at: 2880 4452 2880
Size: 1572 3565
ApaLI G'TGCA_C
Cuts at: 3194 4350 3194
Size: 1156 3981
ApoI r'AATT_y
Cuts at: 1493 2446 2672 2824 4933 1493
Size: 953 226 152 2109 1697
Fragments arranged by size:
2109 1697 953 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3691 160
Size: 872 2659 1606
Fragments arranged by size:
2659 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5137
AvaI C'yCGr_G
Cuts at: 1870 1943 2462 1870
Size: 73 519 4545
Fragments arranged by size:
4545 519 73
BamHI G'GATC_C
Cuts at: 2467 2834 2467
Size: 367 4770
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2458 3913 618
Size: 325 217 627 671 1455 1842
Fragments arranged by size:
1842 1455 671 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2456 1391
Size: 1065 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2151 928
Size: 971 252 3914
Fragments arranged by size:
3914 971 252
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2359 2393 3328 3362 1903
Size: 34 422 34 935 34 3678
Fragments arranged by size:
3678 935 422 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3029 4239 3029
Size: 1210 3927
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5137
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 3748 136
Size: 107 122 71 631 605 337 1739 1525
Fragments arranged by size:
1739 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5132 5132
Size: 5137
BlpI GC'TnA_GC
Cuts at: 2554 2554
Size: 5137
Bme1580I G_kGCm'C
Cuts at: 1790 3198 4354 1790
Size: 1408 1156 2573
Fragments arranged by size:
2573 1408 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5137
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2868 3826 442
Size: 416 53 83 399 24 1451 958 1753
Fragments arranged by size:
1753 1451 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5137
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3797 1239
Size: 2558 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3262 4127 4425 3262
Size: 865 298 3974
Fragments arranged by size:
3974 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3800 882
Size: 617 2301 2219
Fragments arranged by size:
2301 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2928 493
Size: 711 1724 2702
Fragments arranged by size:
2702 1724 711
BsaBI GATnn'nnATC
Cuts at: 5131 5131
Size: 5137
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2944 3326 3961 275
Size: 53 83 186 936 1411 382 635 1451
Fragments arranged by size:
1451 1411 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3570 3968 4242 4389 4800 3570
Size: 398 274 147 411 3907
Fragments arranged by size:
3907 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4912
Fragments arranged by size:
4912 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2117 1186
Size: 931 4206
BseYI C'CCAG_C
Cuts at: 4340 4836 4340
Size: 496 4641
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4564
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2587 3348 4376 1540
Size: 275 186 586 761 1028 2301
Fragments arranged by size:
2301 1028 761 586 275 186
BsiHKAI G_wGCw'C
Cuts at: 3198 3283 4354 3198
Size: 85 1071 3981
Fragments arranged by size:
3981 1071 85
BsmI GAATG_Cn'
Cuts at: 2648 2741 2648
Size: 93 5044
BsmBI CGTCTCn'nnnn_
Cuts at: 2422 2422
Size: 5137
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2456 3198 3283 4354 1391
Size: 399 666 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 666 399 85
BspEI T'CCGG_A
Cuts at: 4800 4800
Size: 5137
BspHI T'CATG_A
Cuts at: 3026 4756 3026
Size: 1730 3407
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2492 844
Size: 1648 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3024 1394
Size: 429 1201 3507
Fragments arranged by size:
3507 1201 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3632 3806 65
Size: 1183 2384 174 1396
Fragments arranged by size:
2384 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3781 3968 1700
Size: 110 1971 187 2869
Fragments arranged by size:
2869 1971 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5137
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5137
BssSI C'ACGA_G
Cuts at: 3197 4209 3197
Size: 1012 4125
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2272 794
Size: 1478 3659
BstBI TT'CG_AA
Cuts at: 3941 3941
Size: 5137
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5137
BstYI r'GATC_y
Cuts at: 1972 2467 2834 3219 3236 4688 4774 4786 5132
Size: 495 367 385 17 1452 86 12 346
Cuts at: 5132 1972
Size: 1977
Fragments arranged by size:
1977 1452 495 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3646
Fragments arranged by size:
3646 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4398
Fragments arranged by size:
4398 683 56
BtsI GCAGTG_nn'
Cuts at: 2283 2653 3447 3467 2283
Size: 370 794 20 3953
Fragments arranged by size:
3953 794 370 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5137
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5102
DraI TTT'AAA
Cuts at: 2441 2441
Size: 5137
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4144 809
Size: 507 2828 1802
Fragments arranged by size:
2828 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2361 2584 3473
Size: 54 1425 50 275 18 531 223 889
Cuts at: 3473 5102 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 531 275 223 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2584 1537
Size: 275 772 4090
Fragments arranged by size:
4090 772 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2109 3067 1353
Size: 756 958 3423
Fragments arranged by size:
3423 958 756
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3724 4098 4244 387
Size: 1064 138 2135 374 146 1280
Fragments arranged by size:
2135 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2236 3200 3797 4584 4852 1239
Size: 393 604 964 597 787 268 1524
Fragments arranged by size:
1524 964 787 604 597 393 268
EcoRI G'AATT_C
Cuts at: 2446 2824 2446
Size: 378 4759
FspI TGC'GCA
Cuts at: 3643 3643
Size: 5137
HaeII r_GCGC'y
Cuts at: 1678 4284 1678
Size: 2606 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3778 3810 3852 3884 1373
Size: 32 135 32 2206 32 42 32 2626
Fragments arranged by size:
2626 2206 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2481 2727 669
Size: 988 281 543 246 3079
Fragments arranged by size:
3079 988 543 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5137
HpaI GTT'AAC
Cuts at: 2727 2727
Size: 5137
KpnI G_GTAC'C
Cuts at: 2462 2462
Size: 5137
MfeI C'AATT_G
Cuts at: 2736 2736
Size: 5137
MluI A'CGCG_T
Cuts at: 5013 5013
Size: 5137
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2010 2335 4251 4435 1305
Size: 705 325 1916 184 2007
Fragments arranged by size:
2007 1916 705 325 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3315
Fragments arranged by size:
3315 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2414 3095 3454 3613 518
Size: 648 1248 681 359 159 2042
Fragments arranged by size:
2042 1248 681 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2553 3230 4378 4623 1606
Size: 947 677 1148 245 2120
Fragments arranged by size:
2120 1148 947 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3646
Fragments arranged by size:
3646 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5137
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5137
NotI GC'GGCC_GC
Cuts at: 2584 2584
Size: 5137
NspI r_CATG'y
Cuts at: 1132 1229 2095 2275 2495 4040 1132
Size: 97 866 180 220 1545 2229
Fragments arranged by size:
2229 1545 866 220 180 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5137
PciI A'CATG_T
Cuts at: 4036 4036
Size: 5137
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5137
PmeI GTTT'AAAC
Cuts at: 2441 2441
Size: 5137
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3206 3238 4719 4751 3206
Size: 32 1481 32 3592
Fragments arranged by size:
3592 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5137
PsiI TTA'TAA
Cuts at: 2707 2707
Size: 5137
PstI C_TGCA'G
Cuts at: 830 2341 2489 830
Size: 1511 148 3478
Fragments arranged by size:
3478 1511 148
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5137
SalI G'TCGA_C
Cuts at: 1936 2479 1936
Size: 543 4594
SbfI CC_TGCA'GG
Cuts at: 2489 2489
Size: 5137
ScaI AGT'ACT
Cuts at: 3385 5097 3385
Size: 1712 3425
SfcI C'TryA_G
Cuts at: 826 1045 2337 2485 3620 4301 4492 826
Size: 219 1292 148 1135 681 191 1471
Fragments arranged by size:
1471 1292 1135 681 219 191 148
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5137
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5137
SmaI CCC'GGG
Cuts at: 1872 2464 1872
Size: 592 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3241 4142 4404 820
Size: 197 926 1298 901 262 1553
Fragments arranged by size:
1553 1298 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2928 493
Size: 2435 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5137
SphI G_CATG'C
Cuts at: 1132 2095 2495 1132
Size: 963 400 3774
Fragments arranged by size:
3774 963 400
SspI AAT'ATT
Cuts at: 5 52 3061 5
Size: 47 3009 2081
Fragments arranged by size:
3009 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5137
StyI C'CwwG_G
Cuts at: 513 1065 2004 2576 513
Size: 552 939 572 3074
Fragments arranged by size:
3074 939 572 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3164 3181 3334 3519 3964 325
Size: 1255 1584 17 153 185 445 1498
Fragments arranged by size:
1584 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3383 5095 96
Size: 274 80 33 51 1443 1406 1712 138
Fragments arranged by size:
1712 1443 1406 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5097 5129 5097
Size: 32 5105
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5137
XbaI T'CTAG_A
Cuts at: 2473 2473
Size: 5137
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5137
XmaI C'CCGG_G
Cuts at: 1870 2462 1870
Size: 592 4545
XmnI GAAnn'nnTTC
Cuts at: 3266 3266
Size: 5137
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2945 276
Size: 53 83 186 936 1411 2468
Fragments arranged by size:
2468 1411 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI