################################## # NO CUT ENZYMES ( 182 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BamHI BanI BanII BbeI BbrPI BbvCI BbvI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaHI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT22I FauI FbaI Fnu4HI FseI FspI HaeII HapII HhaI Hin1I HinP1I HindIII HpaII ItaI KasI KpnI Ksp632I KspI MaeII MfeI MluI MroI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TseI Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AcsI 55 GAATTC [GA]^AATT[CT] AluI 497 AGCT AG^CT AlwI 405 GATCC GGATC(4/5) ApoI 55 GAATTC [GA]^AATT[CT] BalI 15 TGGCCA TGG^CCA BbsI 97 GAAGAC GAAGAC(2/6) BfaI 655 CTAG C^TAG BmyI 246 GTGCCC G[AGT]GC[ACT]^C BpuAI 97 GAAGAC GAAGAC(2/6) BsaBI 405 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaJI 289 CCTTGG C^C[ACGT][ACGT]GG BsiYI 735 CCAACATTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 735 CCAACATTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmI 378 GAATGC GAATGC(1/-1) Bsp1286I 246 GTGCCC G[AGT]GC[ACT]^C BsrDI 621 CATTGC GCAATG(2/0) BssKI 722 CCAGG ^CC[ACGT]GG BstNI 722 CCAGG CC^[AT]GG BstYI 404 AGATCC [GA]^GATC[CT] DraIII 42 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG EcoRI 55 GAATTC G^AATTC EcoRII 722 CCAGG ^CC[AT]GG EcoT14I 289 CCTTGG C^C[AT][AT]GG HgaI 342 GCGTC GACGC(5/10) HincII 304 GTTAAC GT[CT]^[GA]AC HindII 304 GTTAAC GT[CT]^[GA]AC HpaI 304 GTTAAC GTT^AAC HphI 481 GGTGA GGTGA(8/7) MaeI 655 CTAG C^TAG MamI 405 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 404 AGATCC [GA]^GATC[CT] MluNI 15 TGGCCA TGG^CCA MscI 15 TGGCCA TGG^CCA MslI 295 CATGAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvaI 722 CCAGG CC^[AT]GG NdeI 436 CATATG CA^TATG PflMI 735 CCAACATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 100 GACTC GAGTC(4/5) ScaI 116 AGTACT AGT^ACT ScrFI 722 CCAGG CC^[ACGT]GG SfaNI 542 GATGC GCATC(5/9) StyI 289 CCTTGG C^C[AT][AT]GG Van91I 735 CCAACATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 404 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AciI 492 CCGC CCGC(-3/-1) 667 GCGG BsaI 512 GAGACC GGTCTC(1/5) 567 GAGACC BspMI 253 ACCTGC ACCTGC(4/8) 442 ACCTGC Cfr13I 350 GGGCC G^G[ACGT]CC 659 GGGCC DdeI 164 CTCAG C^T[ACGT]AG 529 CTCAG DpnII 221 GATC ^GATC 405 GATC EaeI 15 TGGCCA [CT]^GGCC[GA] 132 TGGCCG MboI 221 GATC ^GATC 405 GATC MboII 64 GAAGA GAAGA(8/7) 97 GAAGA NdeII 221 GATC ^GATC 405 GATC Sau3AI 221 GATC ^GATC 405 GATC Sau96I 350 GGGCC G^G[ACGT]CC 659 GGGCC TfiI 142 GATTC G^A[AT]TC 726 GAATC Tsp45I 344 GTCAC ^GT[GC]AC 431 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 18 enzymes) # ######################################### AfaI BsmAI BsrI BstF5I Cac8I FokI HaeIII HinfI MaeIII MnlI MseI MwoI NlaIII NlaIV RsaI Tru9I Tsp509I TspRI