(Circular) (Six-base) MAPSORT of: pFN21AE3561.seq Check: 2943 from: 1 to: 7483 pFN21AE3561 7483 bp With 182 enzymes: SgfI * December 8, 2014 13:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5293 278 Size: 53 83 186 936 3757 2468 Fragments arranged by size: 3757 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4826 1937 Size: 2889 4594 Acc65I G'GTAC_C Cuts at: 4804 4804 Size: 7483 AclI AA'CG_TT Cuts at: 1563 5610 5983 1563 Size: 4047 373 3063 Fragments arranged by size: 4047 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2721 2810 4118 4682 5546 6930 7198 1632 Size: 1089 89 1308 564 864 1384 268 1917 Fragments arranged by size: 1917 1384 1308 1089 864 564 268 89 AflII C'TTAA_G Cuts at: 820 1017 3548 820 Size: 197 2531 4755 Fragments arranged by size: 4755 2531 197 AflIII A'CryG_T Cuts at: 1203 2712 3977 6382 7359 1203 Size: 1509 1265 2405 977 1327 Fragments arranged by size: 2405 1509 1327 1265 977 AgeI A'CCGG_T Cuts at: 6314 6314 Size: 7483 AhdI GACnn_n'nnGTC Cuts at: 6212 6212 Size: 7483 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7483 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3105 4738 1184 Size: 1921 1633 3929 Fragments arranged by size: 3929 1921 1633 AlwNI CAG_nnn'CTG Cuts at: 5226 6798 5226 Size: 1572 5911 ApaI G_GGCC'C Cuts at: 3721 3721 Size: 7483 ApaLI G'TGCA_C Cuts at: 5540 6696 5540 Size: 1156 6327 ApoI r'AATT_y Cuts at: 1493 2360 2482 2541 3382 3555 4053 4091 4349 Size: 867 122 59 841 173 498 38 258 Cuts at: 4349 4792 5018 5170 7279 1493 Size: 443 226 152 2109 1697 Fragments arranged by size: 2109 1697 867 841 498 443 258 226 173 152 122 59 38 AseI AT'TA_AT Cuts at: 160 1032 4460 6037 160 Size: 872 3428 1577 1606 Fragments arranged by size: 3428 1606 1577 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7483 AvaI C'yCGr_G Cuts at: 1870 1943 4808 1870 Size: 73 2865 4545 Fragments arranged by size: 4545 2865 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4728 4761 4728 Size: 33 7450 BamHI G'GATC_C Cuts at: 4244 4813 5180 4244 Size: 569 367 6547 Fragments arranged by size: 6547 569 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4262 4804 6259 618 Size: 325 217 627 2475 542 1455 1842 Fragments arranged by size: 2475 1842 1455 627 542 325 217 BanII G_rGCy'C Cuts at: 1391 3721 4802 1391 Size: 2330 1081 4072 Fragments arranged by size: 4072 2330 1081 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2166 3496 928 Size: 971 267 1330 4915 Fragments arranged by size: 4915 1330 971 267 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5674 5708 1903 Size: 34 3737 34 3678 Fragments arranged by size: 3737 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5375 6585 5375 Size: 1210 6273 BclI T'GATC_A Cuts at: 1548 2253 1548 Size: 705 6778 BfrBI ATG'CAT Cuts at: 3082 3129 3082 Size: 47 7436 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3164 4289 6094 Size: 107 122 71 631 605 1492 1125 1805 Cuts at: 6094 136 Size: 1525 Fragments arranged by size: 1805 1525 1492 1125 631 605 122 107 71 BglII A'GATC_T Cuts at: 4693 7478 4693 Size: 2785 4698 BlpI GC'TnA_GC Cuts at: 3225 4900 3225 Size: 1675 5808 Bme1580I G_kGCm'C Cuts at: 1790 2574 3721 5544 6700 1790 Size: 784 1147 1823 1156 2573 Fragments arranged by size: 2573 1823 1156 1147 784 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7483 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2297 2568 3039 Size: 416 53 83 399 24 880 271 471 Cuts at: 3039 3724 3821 5214 6172 442 Size: 685 97 1393 958 1753 Fragments arranged by size: 1753 1393 958 880 685 471 416 399 271 97 83 53 24 BmtI G_CTAG'C Cuts at: 1056 4064 1056 Size: 3008 4475 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4032 4032 Size: 7483 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2298 2356 2455 4250 6143 1239 Size: 1059 58 99 1795 1893 2579 Fragments arranged by size: 2579 1893 1795 1059 99 58 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4538 5608 6473 6771 4538 Size: 1070 865 298 5250 Fragments arranged by size: 5250 1070 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6146 882 Size: 617 4647 2219 Fragments arranged by size: 4647 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5274 493 Size: 711 4070 2702 Fragments arranged by size: 4070 2702 711 BsaBI GATnn'nnATC Cuts at: 2639 7477 2639 Size: 4838 2645 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5290 5672 6307 275 Size: 53 83 186 936 3757 382 635 1451 Fragments arranged by size: 3757 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5916 6314 6588 6735 7146 5916 Size: 398 274 147 411 6253 Fragments arranged by size: 6253 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2378 2408 1178 Size: 30 165 30 975 30 6253 Fragments arranged by size: 6253 975 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4253 1186 Size: 3067 4416 BseYI C'CCAG_C Cuts at: 4270 4652 6686 7182 4270 Size: 382 2034 496 4571 Fragments arranged by size: 4571 2034 496 382 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3017 1163 Size: 573 1281 5629 Fragments arranged by size: 5629 1281 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4933 5694 6722 1540 Size: 275 186 2932 761 1028 2301 Fragments arranged by size: 2932 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3438 3734 5544 5629 6700 3438 Size: 296 1810 85 1071 4221 Fragments arranged by size: 4221 1810 1071 296 85 BsmI GAATG_Cn' Cuts at: 2009 3584 4994 5087 2009 Size: 1575 1410 93 4405 Fragments arranged by size: 4405 1575 1410 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2574 3438 3721 3734 4802 5544 5629 Size: 399 784 864 283 13 1068 742 85 Cuts at: 5629 6700 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 1068 864 784 742 399 283 85 13 BspEI T'CCGG_A Cuts at: 7146 7146 Size: 7483 BspHI T'CATG_A Cuts at: 2137 3195 5372 7102 2137 Size: 1058 2177 1730 2518 Fragments arranged by size: 2518 2177 1730 1058 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2039 4838 844 Size: 1195 2799 3489 Fragments arranged by size: 3489 2799 1195 BsrBI CCG'CTC Cuts at: 1394 1823 4629 5370 1394 Size: 429 2806 741 3507 Fragments arranged by size: 3507 2806 741 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2337 2604 4278 4593 4639 5978 6152 Size: 1183 1089 267 1674 315 46 1339 174 Cuts at: 6152 65 Size: 1396 Fragments arranged by size: 1674 1396 1339 1183 1089 315 267 174 46 BsrFI r'CCGG_y Cuts at: 1700 1810 4435 6127 6314 1700 Size: 110 2625 1692 187 2869 Fragments arranged by size: 2869 2625 1692 187 110 BsrGI T'GTAC_A Cuts at: 96 2312 96 Size: 2216 5267 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7483 BssSI C'ACGA_G Cuts at: 3300 3943 5543 6555 3300 Size: 643 1600 1012 4228 Fragments arranged by size: 4228 1600 1012 643 BstAPI GCAn_nnn'nTGC Cuts at: 794 3263 3738 4029 794 Size: 2469 475 291 4248 Fragments arranged by size: 4248 2469 475 291 BstBI TT'CG_AA Cuts at: 6287 6287 Size: 7483 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7483 BstYI r'GATC_y Cuts at: 1972 3286 4244 4693 4813 5180 5565 5582 7034 Size: 1314 958 449 120 367 385 17 1452 Cuts at: 7034 7120 7132 7478 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1314 958 449 385 367 346 120 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3332 513 Size: 552 939 1328 4664 Fragments arranged by size: 4664 1328 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6744 Fragments arranged by size: 6744 683 56 BtsI GCAGTG_nn' Cuts at: 3264 3685 4999 5793 5813 3264 Size: 421 1314 794 20 4934 Fragments arranged by size: 4934 1314 794 421 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7483 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7448 DraI TTT'AAA Cuts at: 3414 4787 3414 Size: 1373 6110 DraIII CAC_nnn'GTG Cuts at: 3738 3738 Size: 7483 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6490 809 Size: 507 5174 1802 Fragments arranged by size: 5174 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3783 4930 5819 Size: 54 1425 50 275 18 1953 1147 889 Cuts at: 5819 7448 8 Size: 1629 43 Fragments arranged by size: 1953 1629 1425 1147 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4930 1537 Size: 275 3118 4090 Fragments arranged by size: 4090 3118 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2867 2986 5413 1353 Size: 1514 119 2427 3423 Fragments arranged by size: 3423 2427 1514 119 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6070 6444 6590 387 Size: 1064 138 4481 374 146 1280 Fragments arranged by size: 4481 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2298 2356 2455 2721 2810 4118 4250 Size: 393 666 58 99 266 89 1308 132 Cuts at: 4250 4682 5546 6143 6930 7198 1239 Size: 432 864 597 787 268 1524 Fragments arranged by size: 1524 1308 864 787 666 597 432 393 268 266 132 99 89 58 EcoO109I rG'GnC_Cy Cuts at: 3718 3718 Size: 7483 EcoRI G'AATT_C Cuts at: 3382 4792 5170 3382 Size: 1410 378 5695 Fragments arranged by size: 5695 1410 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2753 4564 2753 Size: 1811 5672 FspI TGC'GCA Cuts at: 3097 5989 3097 Size: 2892 4591 HaeII r_GCGC'y Cuts at: 1678 6630 1678 Size: 4952 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2166 2198 2903 2935 3882 Size: 32 135 32 594 32 705 32 947 Cuts at: 3882 3914 4030 4062 4209 4229 4241 4261 6124 Size: 32 116 32 147 20 12 20 1863 Cuts at: 6124 6156 6198 6230 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1863 947 705 594 147 135 116 42 32 32 32 32 32 32 32 32 20 20 12 HincII GTy'rAC Cuts at: 669 1657 1938 2922 3617 4827 5073 669 Size: 988 281 984 695 1210 246 3079 Fragments arranged by size: 3079 1210 988 984 695 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7483 HpaI GTT'AAC Cuts at: 5073 5073 Size: 7483 KpnI G_GTAC'C Cuts at: 4808 4808 Size: 7483 MfeI C'AATT_G Cuts at: 5082 5082 Size: 7483 MluI A'CGCG_T Cuts at: 7359 7359 Size: 7483 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2606 6597 6781 1305 Size: 1301 3991 184 2007 Fragments arranged by size: 3991 2007 1301 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3785 10 Size: 54 1425 343 1953 3708 Fragments arranged by size: 3708 1953 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2249 2879 3072 3396 3473 4720 5441 Size: 648 1083 630 193 324 77 1247 721 Cuts at: 5441 5800 5959 518 Size: 359 159 2042 Fragments arranged by size: 2042 1247 1083 721 648 630 359 324 193 159 77 MspA1I CmG'CkG Cuts at: 1606 4899 5576 6724 6969 1606 Size: 3293 677 1148 245 2120 Fragments arranged by size: 3293 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3332 513 Size: 552 939 1328 4664 Fragments arranged by size: 4664 1328 939 552 NdeI CA'TA_TG Cuts at: 387 3100 4721 387 Size: 2713 1621 3149 Fragments arranged by size: 3149 2713 1621 NheI G'CTAG_C Cuts at: 1052 4060 1052 Size: 3008 4475 NotI GC'GGCC_GC Cuts at: 4930 4930 Size: 7483 NsiI A_TGCA'T Cuts at: 3084 3131 3084 Size: 47 7436 NspI r_CATG'y Cuts at: 1132 1229 2416 3981 4369 4841 6386 1132 Size: 97 1187 1565 388 472 1545 2229 Fragments arranged by size: 2229 1565 1545 1187 472 388 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7483 PciI A'CATG_T Cuts at: 3977 6382 3977 Size: 2405 5078 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7483 PfoI T'CCnGG_A Cuts at: 2101 2184 2471 4227 2101 Size: 83 287 1756 5357 Fragments arranged by size: 5357 1756 287 83 PmeI GTTT'AAAC Cuts at: 4787 4787 Size: 7483 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5552 5584 7065 7097 5552 Size: 32 1481 32 5938 Fragments arranged by size: 5938 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7483 PsiI TTA'TAA Cuts at: 2690 5053 2690 Size: 2363 5120 PspOMI G'GGCC_C Cuts at: 3717 3717 Size: 7483 PstI C_TGCA'G Cuts at: 830 2524 4835 830 Size: 1694 2311 3478 Fragments arranged by size: 3478 2311 1694 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7483 SalI G'TCGA_C Cuts at: 1936 4825 1936 Size: 2889 4594 SbfI CC_TGCA'GG Cuts at: 4835 4835 Size: 7483 ScaI AGT'ACT Cuts at: 5731 7443 5731 Size: 1712 5771 SfcI C'TryA_G Cuts at: 826 1045 2520 3177 3491 4108 4191 4564 4831 Size: 219 1475 657 314 617 83 373 267 Cuts at: 4831 5966 6647 6838 826 Size: 1135 681 191 1471 Fragments arranged by size: 1475 1471 1135 681 657 617 373 314 267 219 191 83 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7483 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7483 SmaI CCC'GGG Cuts at: 1872 4810 1872 Size: 2938 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3548 4553 5587 6488 6750 820 Size: 197 926 1605 1005 1034 901 262 1553 Fragments arranged by size: 1605 1553 1034 1005 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5274 493 Size: 4781 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7483 SphI G_CATG'C Cuts at: 1132 4369 4841 1132 Size: 3237 472 3774 Fragments arranged by size: 3774 3237 472 SspI AAT'ATT Cuts at: 5 52 3846 5407 5 Size: 47 3794 1561 2081 Fragments arranged by size: 3794 2081 1561 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7483 StyI C'CwwG_G Cuts at: 513 1065 2004 3268 3332 3573 3780 4922 513 Size: 552 939 1264 64 241 207 1142 3074 Fragments arranged by size: 3074 1264 1142 939 552 241 207 64 SwaI ATTT'AAAT Cuts at: 3414 3414 Size: 7483 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4129 5510 5527 5680 5865 6310 325 Size: 1255 2549 1381 17 153 185 445 1498 Fragments arranged by size: 2549 1498 1381 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2312 5729 7441 Size: 274 80 33 51 1443 335 3417 1712 Cuts at: 7441 96 Size: 138 Fragments arranged by size: 3417 1712 1443 335 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2378 2410 2893 2925 7443 7475 2378 Size: 32 483 32 4518 32 2386 Fragments arranged by size: 4518 2386 483 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4042 1316 Size: 2726 4757 XbaI T'CTAG_A Cuts at: 4819 4819 Size: 7483 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2192 2380 2192 Size: 188 7295 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7483 XmaI C'CCGG_G Cuts at: 1870 4808 1870 Size: 2938 4545 XmnI GAAnn'nnTTC Cuts at: 5612 5612 Size: 7483 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5291 276 Size: 53 83 186 936 3757 2468 Fragments arranged by size: 3757 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BbvCI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI