################################## # NO CUT ENZYMES ( 155 enzymes) # ################################## AatII Acc65I AccII AccIII AciI AclI AcyI AflII AgeI AhdI Alw44I AlwNI Aor51HI ApaLI AscI Asp718I AspEI AspI AvaI AviII AvrII BalI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BcnI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmFI BsoBI BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I CelII CfoI Cfr10I ClaI CpoI DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MfeI MluI MluNI MroI MscI MspA1I MspI MunI MvnI NaeI NarI NciI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I StuI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AccI 83 GTAGAC GT^[AC][GT]AC AflIII 821 ACGTGT A^C[GA][CT]GT AocI 1546 CCTCAGG CC^T[ACGT]AGG AseI 1396 ATTAAT AT^TAAT AsnI 1396 ATTAAT AT^TAAT AvaII 853 GGACC G^G[AT]CC BamHI 204 GGATCC G^GATCC BclI 291 TGATCA T^GATCA BfaI 14 CTAG C^TAG BglI 697 GCCGTGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaBI 519 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseRI 737 CTCCTC GAGGAG(10/8) BsiYI 1597 CCAATGGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 1597 CCAATGGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 1028 GTCTC GTCTC(1/5) BsmI 607 GAATGC GAATGC(1/-1) Bsp1407I 384 TGTACA T^GTACA BspMI 945 ACCTGC ACCTGC(4/8) BsrGI 384 TGTACA T^GTACA BstYI 204 GGATCC [GA]^GATC[CT] Bsu36I 1546 CCTCAGG CC^T[ACGT]AGG Cac8I 1075 GCCTGC GC[ACGT]^[ACGT]GC DraI 1332 TTTAAA TTT^AAA DraIII 1283 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 8 CCATGG C^C[GA][CT]GG EarI 89 GAAGAG CTCTTC(1/4) Eco81I 1546 CCTCAGG CC^T[ACGT]AGG FbaI 291 TGATCA T^GATCA Ksp632I 89 GAAGAG CTCTTC(1/4) MaeI 14 CTAG C^TAG MamI 519 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 204 GGATCC [GA]^GATC[CT] MslI 547 CATGAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NcoI 8 CCATGG C^CATGG NdeI 1427 CATATG CA^TATG PflMI 1597 CCAATGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshBI 1396 ATTAAT AT^TAAT SacI 1112 GAGCTC GAGCT^C SmlI 71 CTCAAG C^T[CT][GA]AG SspBI 384 TGTACA T^GTACA TaqI 897 TCGA T^CGA TthHB8I 897 TCGA T^CGA Van91I 1597 CCAATGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 204 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AlwI 205 GATCC GGATC(4/5) 204 GGATC ApaI 704 GGGCCC GGGCC^C 830 GGGCCC Asp700I 550 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 634 GAAATACTTC AspHI 1112 GAGCTC G[AT]GC[AT]^C 1409 GTGCTC BpmI 1238 CTCCAG CTGGAG(16/14) 887 CTGGAG BsiHKAI 1112 GAGCTC G[AT]GC[AT]^C 1409 GTGCTC BsrI 505 ACTGG ACTGG(1/-1) 976 ACTGG BssKI 19 CCAGG ^CC[ACGT]GG 376 CCAGG BstNI 19 CCAGG CC^[AT]GG 376 CCAGG DraII 704 GGGCCCT [GA]G^G[ACGT]CC[CT] 830 GGGCCCT EcoO109I 704 GGGCCCT [GA]G^G[ACGT]CC[CT] 830 GGGCCCT EcoRII 19 CCAGG ^CC[AT]GG 376 CCAGG EcoT14I 8 CCATGG C^C[AT][AT]GG 488 CCAAGG HaeIII 705 GGCC GG^CC 831 GGCC MaeII 821 ACGT A^CGT 1356 ACGT MvaI 19 CCAGG CC^[AT]GG 376 CCAGG ScrFI 19 CCAGG CC^[ACGT]GG 376 CCAGG SfcI 33 CTATAG C^T[GA][CT]AG 254 CTACAG StyI 8 CCATGG C^C[AT][AT]GG 488 CCAAGG XmnI 550 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 634 GAAATACTTC ######################################### # 3 OR MORE CUT ENZYMES ( 39 enzymes) # ######################################### AcsI AfaI AluI ApoI BanII BbvI BmyI BsaJI Bsp1286I BstF5I Cfr13I DdeI DpnII Fnu4HI FokI HinfI HphI ItaI MaeIII MboI MboII MnlI MseI MwoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I SfaNI SspI TfiI Tru9I TseI Tsp45I Tsp509I TspRI