Restriction map for
pFN21AE4511
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# NO CUT ENZYMES ( 155 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AgeI AhdI
Alw44I AlwNI Aor51HI ApaLI AscI
Asp718I AspEI AspI AvaI AviII
AvrII BalI BanI BbeI BbrPI
BbsI BbvCI BcgI BciVI BcnI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsmFI BsoBI BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I CelII
CfoI Cfr10I ClaI CpoI DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FauI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI HpaII
KasI KpnI KspI MfeI MluI
MluNI MroI MscI MspA1I MspI
MunI MvnI NaeI NarI NciI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PstI PvuI PvuII
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I StuI SwaI Tth111I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccI 83 GTAGAC GT^[AC][GT]AC
AflIII 821 ACGTGT A^C[GA][CT]GT
AocI 1546 CCTCAGG CC^T[ACGT]AGG
AseI 1396 ATTAAT AT^TAAT
AsnI 1396 ATTAAT AT^TAAT
AvaII 853 GGACC G^G[AT]CC
BamHI 204 GGATCC G^GATCC
BclI 291 TGATCA T^GATCA
BfaI 14 CTAG C^TAG
BglI 697 GCCGTGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 519 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 737 CTCCTC GAGGAG(10/8)
BsiYI 1597 CCAATGGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 1597 CCAATGGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 1028 GTCTC GTCTC(1/5)
BsmI 607 GAATGC GAATGC(1/-1)
Bsp1407I 384 TGTACA T^GTACA
BspMI 945 ACCTGC ACCTGC(4/8)
BsrGI 384 TGTACA T^GTACA
BstYI 204 GGATCC [GA]^GATC[CT]
Bsu36I 1546 CCTCAGG CC^T[ACGT]AGG
Cac8I 1075 GCCTGC GC[ACGT]^[ACGT]GC
DraI 1332 TTTAAA TTT^AAA
DraIII 1283 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 8 CCATGG C^C[GA][CT]GG
EarI 89 GAAGAG CTCTTC(1/4)
Eco81I 1546 CCTCAGG CC^T[ACGT]AGG
FbaI 291 TGATCA T^GATCA
Ksp632I 89 GAAGAG CTCTTC(1/4)
MaeI 14 CTAG C^TAG
MamI 519 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 204 GGATCC [GA]^GATC[CT]
MslI 547 CATGAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 8 CCATGG C^CATGG
NdeI 1427 CATATG CA^TATG
PflMI 1597 CCAATGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 1396 ATTAAT AT^TAAT
SacI 1112 GAGCTC GAGCT^C
SmlI 71 CTCAAG C^T[CT][GA]AG
SspBI 384 TGTACA T^GTACA
TaqI 897 TCGA T^CGA
TthHB8I 897 TCGA T^CGA
Van91I 1597 CCAATGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 204 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
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AlwI 205 GATCC GGATC(4/5)
204 GGATC
ApaI 704 GGGCCC GGGCC^C
830 GGGCCC
Asp700I 550 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
634 GAAATACTTC
AspHI 1112 GAGCTC G[AT]GC[AT]^C
1409 GTGCTC
BpmI 1238 CTCCAG CTGGAG(16/14)
887 CTGGAG
BsiHKAI 1112 GAGCTC G[AT]GC[AT]^C
1409 GTGCTC
BsrI 505 ACTGG ACTGG(1/-1)
976 ACTGG
BssKI 19 CCAGG ^CC[ACGT]GG
376 CCAGG
BstNI 19 CCAGG CC^[AT]GG
376 CCAGG
DraII 704 GGGCCCT [GA]G^G[ACGT]CC[CT]
830 GGGCCCT
EcoO109I 704 GGGCCCT [GA]G^G[ACGT]CC[CT]
830 GGGCCCT
EcoRII 19 CCAGG ^CC[AT]GG
376 CCAGG
EcoT14I 8 CCATGG C^C[AT][AT]GG
488 CCAAGG
HaeIII 705 GGCC GG^CC
831 GGCC
MaeII 821 ACGT A^CGT
1356 ACGT
MvaI 19 CCAGG CC^[AT]GG
376 CCAGG
ScrFI 19 CCAGG CC^[ACGT]GG
376 CCAGG
SfcI 33 CTATAG C^T[GA][CT]AG
254 CTACAG
StyI 8 CCATGG C^C[AT][AT]GG
488 CCAAGG
XmnI 550 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
634 GAAATACTTC
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# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AcsI AfaI AluI ApoI BanII
BbvI BmyI BsaJI Bsp1286I BstF5I
Cfr13I DdeI DpnII Fnu4HI FokI
HinfI HphI ItaI MaeIII MboI
MboII MnlI MseI MwoI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I SfaNI SspI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI