##################################
# NO CUT ENZYMES (  208 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwI        
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp718I     
AspEI       AspHI       AspI        AvaI        AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BclI        BcnI        BfrI        BglI        BglII       
BlnI        BmyI        BpmI        BsaAI       BsaBI       
BsaHI       BsaI        BsaJI       BsaWI       BseAI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsmBI       
BsmI        BsoBI       Bsp1286I    Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrDI       
BsrFI       BsrGI       BsrI        BssHII      BssKI       
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstF5I      BstNI       BstPI       BstUI       BstXI       
BstYI       BstZ17I     Bsu36I      Cac8I       CfoI        
Cfr10I      ClaI        CpoI        DpnII       DraI        
DraIII      DsaI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRI       EcoRII      EcoRV       EcoT14I     
EcoT22I     FauI        FbaI        FokI        FseI        
FspI        HaeII       HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HpaI        HpaII       HphI        KasI        KpnI        
KspI        MaeII       MamI        MboI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MseI        MspI        MunI        MvaI        MvnI        
NaeI        NarI        NciI        NcoI        NdeI        
NdeII       NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PstI        PvuI        
RcaI        RsaI        RsrII       SacI        SacII       
SalI        SapI        Sau3AI      SbfI        ScaI        
ScrFI       SexAI       SfaNI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        Tru9I       
Tsp45I      Tth111I     Van91I      XbaI        XhoI        
XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   27 enzymes) #
###################################

Asp700I       496  GAAGAGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BfaI          208  CTAG                 C^TAG
BlpI          306  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      306  GCTCAGC              GC^T[ACGT]AGC
BsiYI          28  CCAGAGGACGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI           28  CCAGAGGACGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI         598  GAGAC                GTCTC(1/5)
BsmFI         211  GGGAC                GGGAC(10/14)
BsrBI          19  GAGCGG               CCGCTC(-3/-3)
CelII         306  GCTCAGC              GC^T[ACGT]AGC
DraII         211  GGGACCT              [GA]G^G[ACGT]CC[CT]
DrdI          172  GACAGCTGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I      211  GGGACCT              [GA]G^G[ACGT]CC[CT]
HaeIII        155  GGCC                 GG^CC
MaeI          208  CTAG                 C^TAG
MaeIII        422  GTAAC                ^GT[ACGT]AC
MslI          433  CATGAAAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        174  CAGCTG               C[AC]G^C[GT]G
NlaIV         211  GGGACC               GG[ACGT]^[ACGT]CC
NspI          243  ACATGC               [GA]CATG^[CT]
PleI           24  GAGTC                GAGTC(4/5)
PpuMI         211  GGGACCT              [GA]G^G[AT]CC[CT]
PvuII         174  CAGCTG               CAG^CTG
TaqI          405  TCGA                 T^CGA
TthHB8I       405  TCGA                 T^CGA
XcmI          158  CCATTCTGCAAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI          496  GAAGAGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (    6 enzymes) #
###################################

AvaII         212  GGACC                G^G[AT]CC
              574  GGTCC               
BbvI          375  GCTGC                GCAGC(8/12)
              633  GCTGC               
BseRI          52  GAGGAG               GAGGAG(10/8)
               55  GAGGAG              
MwoI            4  GCGGCGGCGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              156  GCCCATTCTGC         
TseI          375  GCTGC                G^C[AT]GC
              633  GCTGC               
TspRI         147  CACTG                CA[GC]TG[ACGT][ACGT]^
              272  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   17 enzymes) #
#########################################

AciI        AluI        BbsI        BpuAI       Cfr13I      
DdeI        EarI        Fnu4HI      HinfI       ItaI        
Ksp632I     MboII       MnlI        NlaIII      Sau96I      
TfiI        Tsp509I