Restriction map for
pFN21AB7063
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# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI AocI Aor51HI ApaI
AscI AseI AsnI Asp700I Asp718I
AviII AvrII BalI BamHI BbeI
BbrPI BciVI BfrI BlnI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiWI BsmI Bsp1407I BspDI BspEI
BsrGI BssHII BssSI BstBI Bsu36I
ClaI CpoI DraI DraIII DrdI
Eco47III Eco81I EcoNI EcoRV EcoT22I
FseI FspI HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
MamI MfeI MluI MluNI MroI
MscI MunI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PshBI Psp1406I RsrII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SphI Sse8387I
SspBI SspI SwaI Van91I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AflIII 1841 ACATGT A^C[GA][CT]GT
Alw44I 1083 GTGCAC G^TGCAC
ApaLI 1083 GTGCAC G^TGCAC
AspI 1458 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbsI 190 GAAGAC GAAGAC(2/6)
BbvCI 385 CCTCAGC CCTCAGC(-5/-2)
BcgI 1295 GCACGGTGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 494 TGATCA T^GATCA
BglI 337 GCCACCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 545 AGATCT A^GATCT
BpmI 1408 CTCCAG CTGGAG(16/14)
BpuAI 190 GAAGAC GAAGAC(2/6)
BsaAI 93 TACGTG [CT]AC^GT[GA]
BsmBI 148 GAGACG CGTCTC(1/5)
BspHI 1451 TCATGA T^CATGA
BspLU11I 1841 ACATGT A^CATGT
BsrDI 1535 GCAATG GCAATG(2/0)
BstEII 1396 GGTAACC G^GT[ACGT]ACC
BstPI 1396 GGTAACC G^GT[ACGT]ACC
BstXI 1823 CCAGCGACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 545 AGATCT [GA]^GATC[CT]
DraII 1412 AGGACCT [GA]G^G[ACGT]CC[CT]
EagI 152 CGGCCG C^GGCCG
EclXI 152 CGGCCG C^GGCCG
Eco52I 152 CGGCCG C^GGCCG
EcoO109I 1412 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1396 GGTAACC G^GT[ACGT]ACC
FbaI 494 TGATCA T^GATCA
KspI 341 CCGCGG CCGC^GG
MflI 545 AGATCT [GA]^GATC[CT]
NspI 1841 ACATGT [GA]CATG^[CT]
PflFI 1458 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1412 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 844 GACGACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 233 CGATCG CGAT^CG
PvuII 839 CAGCTG CAG^CTG
RcaI 1451 TCATGA T^CATGA
SacII 341 CCGCGG CCGC^GG
SapI 723 GAAGAGC GCTCTTC(1/4)
SpeI 1783 ACTAGT A^CTAGT
StuI 1991 AGGCCT AGG^CCT
Tth111I 1458 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1620 CCAGGGCGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 545 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 777 GTATAC GT^[AC][GT]AC
1245 GTATAC
AcsI 1509 GAATTC [GA]^AATT[CT]
1653 GAATTC
AhdI 319 GACCCCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1867 GACATGGAGTC
AlwNI 370 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG
1411 CAGGACCTG
ApoI 1509 GAATTC [GA]^AATT[CT]
1653 GAATTC
AspEI 319 GACCCCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1867 GACATGGAGTC
BfaI 1235 CTAG C^TAG
1784 CTAG
BsiEI 152 CGGCCG CG[GA][CT]^CG
233 CGATCG
Bst1107I 777 GTATAC GTA^TAC
1245 GTATAC
BstAPI 390 GCAACCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1076 GCAAGCCGTGC
BstZ17I 777 GTATAC GTA^TAC
1245 GTATAC
Eam1105I 319 GACCCCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1867 GACATGGAGTC
EarI 1377 CTCTTC CTCTTC(1/4)
723 GAAGAG
EcoRI 1509 GAATTC G^AATTC
1653 GAATTC
HaeII 372 GGCGCT [GA]GCGC^[CT]
1624 GGCGCT
HphI 497 TCACC GGTGA(8/7)
1063 TCACC
Ksp632I 1377 CTCTTC CTCTTC(1/4)
723 GAAGAG
MaeI 1235 CTAG C^TAG
1784 CTAG
MseI 1289 TTAA T^TAA
1919 TTAA
MslI 1441 CACAGCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1491 CACCGCTGTG
NaeI 217 GCCGGC GCC^GGC
610 GCCGGC
NcoI 227 CCATGG C^CATGG
758 CCATGG
NgoMIV 217 GCCGGC G^CCGGC
610 GCCGGC
PstI 379 CTGCAG CTGCA^G
820 CTGCAG
SmaI 293 CCCGGG CCC^GGG
322 CCCGGG
SmlI 280 CTTGAG C^T[CT][GA]AG
1674 CTTGAG
Tru9I 1289 TTAA T^TAA
1919 TTAA
XmaCI 293 CCCGGG C^CCGGG
322 CCCGGG
XmaI 293 CCCGGG C^CCGGG
322 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI AvaI AvaII BanI BanII
BbvI BcnI BlpI BmyI Bpu1102I
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BstF5I
BstNI BstUI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DsaI
EaeI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII ItaI MaeII MaeIII
MboI MboII MnlI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI TseI Tsp45I
Tsp509I TspRI TthHB8I