(Circular) (Six-base) MAPSORT of: pFN21AB7063.seq Check: 5868 from: 1 to: 6724 pFN21AB7063 6724 bp With 182 enzymes: SgfI * August 16, 2010 14:56 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4534 278 Size: 53 83 186 936 2998 2468 Fragments arranged by size: 2998 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2783 3251 4067 1937 Size: 846 468 816 4594 Fragments arranged by size: 4594 846 816 468 Acc65I G'GTAC_C Cuts at: 4045 4045 Size: 6724 AclI AA'CG_TT Cuts at: 1563 4851 5224 1563 Size: 3288 373 3063 Fragments arranged by size: 3288 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3057 4787 6171 6439 1632 Size: 1425 1730 1384 268 1917 Fragments arranged by size: 1917 1730 1425 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6527 AflIII A'CryG_T Cuts at: 1203 3846 5623 6600 1203 Size: 2643 1777 977 1327 Fragments arranged by size: 2643 1777 1327 977 AgeI A'CCGG_T Cuts at: 5555 5555 Size: 6724 AhdI GACnn_n'nnGTC Cuts at: 2329 3877 5453 2329 Size: 1548 1576 3600 Fragments arranged by size: 3600 1576 1548 AleI CACnn'nnGTG Cuts at: 1166 3500 1166 Size: 2334 4390 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6724 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2051 2083 2051 Size: 32 6692 AlwNI CAG_nnn'CTG Cuts at: 2380 3421 4467 6039 2380 Size: 1041 1046 1572 3065 Fragments arranged by size: 3065 1572 1046 1041 ApaLI G'TGCA_C Cuts at: 3088 4781 5937 3088 Size: 1693 1156 3875 Fragments arranged by size: 3875 1693 1156 ApoI r'AATT_y Cuts at: 1493 3514 3658 4033 4259 4411 6520 1493 Size: 2021 144 375 226 152 2109 1697 Fragments arranged by size: 2109 2021 1697 375 226 152 144 AseI AT'TA_AT Cuts at: 160 1032 5278 160 Size: 872 4246 1606 Fragments arranged by size: 4246 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6724 AvaI C'yCGr_G Cuts at: 1870 1943 2146 2177 2298 2327 3265 4049 1870 Size: 73 203 31 121 29 938 784 4545 Fragments arranged by size: 4545 938 784 203 121 73 31 29 BamHI G'GATC_C Cuts at: 4054 4421 4054 Size: 367 6357 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2014 2308 3125 4015 4045 Size: 325 217 627 227 294 817 890 30 Cuts at: 4045 5500 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 890 817 627 325 294 227 217 30 BanII G_rGCy'C Cuts at: 1391 2076 2124 2135 2273 2435 2522 2699 3239 Size: 685 48 11 138 162 87 177 540 Cuts at: 3239 3266 3278 4043 1391 Size: 27 12 765 4072 Fragments arranged by size: 4072 765 685 540 177 162 138 87 48 27 12 11 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2202 928 Size: 971 303 5450 Fragments arranged by size: 5450 971 303 BbvCI CC'TCA_GC Cuts at: 2391 2391 Size: 6724 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3289 3323 4915 4949 1903 Size: 34 1352 34 1592 34 3678 Fragments arranged by size: 3678 1592 1352 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4616 5826 4616 Size: 1210 5514 BclI T'GATC_A Cuts at: 1548 2499 1548 Size: 951 5773 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2348 5335 136 Size: 107 122 71 631 605 676 2987 1525 Fragments arranged by size: 2987 1525 676 631 605 122 107 71 BglII A'GATC_T Cuts at: 2550 6719 2550 Size: 4169 2555 BlpI GC'TnA_GC Cuts at: 2360 3055 3990 4141 2360 Size: 695 935 151 4943 Fragments arranged by size: 4943 935 695 151 Bme1580I G_kGCm'C Cuts at: 1790 2580 3092 4785 5941 1790 Size: 790 512 1693 1156 2573 Fragments arranged by size: 2573 1693 1156 790 512 BmgBI CAC'GTC Cuts at: 1318 2892 1318 Size: 1574 5150 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3225 4455 5413 Size: 416 53 83 399 24 1808 1230 958 Cuts at: 5413 442 Size: 1753 Fragments arranged by size: 1808 1753 1230 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6724 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3398 5384 1239 Size: 2159 1986 2579 Fragments arranged by size: 2579 2159 1986 Bpu10I CC'TnA_GC Cuts at: 2391 2391 Size: 6724 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2306 3700 4849 5714 6012 2306 Size: 1394 1149 865 298 3018 Fragments arranged by size: 3018 1394 1149 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5387 882 Size: 617 3888 2219 Fragments arranged by size: 3888 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2100 4515 493 Size: 711 896 2415 2702 Fragments arranged by size: 2702 2415 896 711 BsaBI GATnn'nnATC Cuts at: 6718 6718 Size: 6724 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4531 4913 5548 275 Size: 53 83 186 936 2998 382 635 1451 Fragments arranged by size: 2998 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5157 5555 5829 5976 6387 5157 Size: 398 274 147 411 5494 Fragments arranged by size: 5494 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2843 2873 1178 Size: 30 165 30 1440 30 5029 Fragments arranged by size: 5029 1440 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2195 2198 3763 1186 Size: 1009 3 1565 4147 Fragments arranged by size: 4147 1565 1009 3 BseYI C'CCAG_C Cuts at: 2266 2426 2862 3201 3567 3827 4011 5927 6423 Size: 160 436 339 366 260 184 1916 496 Cuts at: 6423 2266 Size: 2567 Fragments arranged by size: 2567 1916 496 436 366 339 260 184 160 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6151 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2160 2241 4174 4935 5963 1540 Size: 275 186 159 81 1933 761 1028 2301 Fragments arranged by size: 2301 1933 1028 761 275 186 159 81 BsiHKAI G_wGCw'C Cuts at: 2124 2522 2699 3092 3239 4785 4870 5941 2124 Size: 398 177 393 147 1546 85 1071 2907 Fragments arranged by size: 2907 1546 1071 398 393 177 147 85 BsmI GAATG_Cn' Cuts at: 4235 4328 4235 Size: 93 6631 BsmBI CGTCTCn'nnnn_ Cuts at: 2147 2147 Size: 6724 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2076 2124 2135 2273 2435 2522 2580 Size: 399 286 48 11 138 162 87 58 Cuts at: 2580 2699 3092 3239 3266 3278 4043 4785 4870 Size: 119 393 147 27 12 765 742 85 Cuts at: 4870 5941 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 765 742 399 393 286 162 147 138 119 87 85 58 48 27 12 11 BspEI T'CCGG_A Cuts at: 6387 6387 Size: 6724 BspHI T'CATG_A Cuts at: 3456 4613 6343 3456 Size: 1157 1730 3837 Fragments arranged by size: 3837 1730 1157 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3180 3429 3807 4079 844 Size: 2336 249 378 272 3489 Fragments arranged by size: 3489 2336 378 272 249 BsrBI CCG'CTC Cuts at: 1394 1823 2035 2044 2259 2364 2733 4611 1394 Size: 429 212 9 215 105 369 1878 3507 Fragments arranged by size: 3507 1878 429 369 215 212 105 9 BsrDI GCAATG_nn' Cuts at: 65 1248 3547 5219 5393 65 Size: 1183 2299 1672 174 1396 Fragments arranged by size: 2299 1672 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2017 2222 2615 5368 5555 1700 Size: 110 207 205 393 2753 187 2869 Fragments arranged by size: 2869 2753 393 207 205 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6724 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6724 BssSI C'ACGA_G Cuts at: 4784 5796 4784 Size: 1012 5712 BstAPI GCAn_nnn'nTGC Cuts at: 794 2401 3087 794 Size: 1607 686 4431 Fragments arranged by size: 4431 1607 686 BstBI TT'CG_AA Cuts at: 5528 5528 Size: 6724 BstEII G'GTnAC_C Cuts at: 1184 3401 1184 Size: 2217 4507 BstXI CCAn_nnnn'nTGG Cuts at: 3835 3835 Size: 6724 BstYI r'GATC_y Cuts at: 1972 2550 4054 4421 4806 4823 6275 6361 6373 Size: 578 1504 367 385 17 1452 86 12 Cuts at: 6373 6719 1972 Size: 346 1977 Fragments arranged by size: 1977 1504 1452 578 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2784 3252 2784 Size: 468 6256 BtgI C'CryG_G Cuts at: 513 1065 2004 2232 2346 2763 2794 3104 513 Size: 552 939 228 114 417 31 310 4133 Fragments arranged by size: 4133 939 552 417 310 228 114 31 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3192 3465 487 Size: 683 56 1966 273 3746 Fragments arranged by size: 3746 1966 683 273 56 BtsI GCAGTG_nn' Cuts at: 2648 2908 4240 5034 5054 2648 Size: 260 1332 794 20 4318 Fragments arranged by size: 4318 1332 794 260 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6724 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2776 2811 529 Size: 35 2212 35 4442 Fragments arranged by size: 4442 2212 35 35 DraI TTT'AAA Cuts at: 4028 4028 Size: 6724 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5731 809 Size: 507 4415 1802 Fragments arranged by size: 4415 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2157 2797 2968 Size: 54 1425 50 275 18 327 640 171 Cuts at: 2968 4171 5060 6689 8 Size: 1203 889 1629 43 Fragments arranged by size: 1629 1425 1203 889 640 327 275 171 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2157 4171 1537 Size: 275 345 2014 4090 Fragments arranged by size: 4090 2014 345 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2723 3388 4654 1353 Size: 1370 665 1266 3423 Fragments arranged by size: 3423 1370 1266 665 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2479 5311 5685 5831 387 Size: 1064 138 890 2832 374 146 1280 Fragments arranged by size: 2832 1280 1064 890 374 146 138 EcoICRI GAG'CTC Cuts at: 2122 2520 2697 3237 2122 Size: 398 177 540 5609 Fragments arranged by size: 5609 540 398 177 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3057 3398 4787 5384 6171 6439 1239 Size: 393 1425 341 1389 597 787 268 1524 Fragments arranged by size: 1524 1425 1389 787 597 393 341 268 EcoO109I rG'GnC_Cy Cuts at: 3418 3418 Size: 6724 EcoRI G'AATT_C Cuts at: 3514 3658 4033 4411 3514 Size: 144 375 378 5827 Fragments arranged by size: 5827 378 375 144 FspI TGC'GCA Cuts at: 5230 5230 Size: 6724 HaeII r_GCGC'y Cuts at: 1678 2381 3633 5871 1678 Size: 703 1252 2238 2531 Fragments arranged by size: 2531 2238 1252 703 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2315 2347 2843 2875 3749 Size: 32 135 32 743 32 496 32 874 Cuts at: 3749 3781 3863 3895 5365 5397 5439 5471 1373 Size: 32 82 32 1470 32 42 32 2626 Fragments arranged by size: 2626 1470 874 743 496 135 82 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4068 4314 669 Size: 988 281 2130 246 3079 Fragments arranged by size: 3079 2130 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6724 HpaI GTT'AAC Cuts at: 4314 4314 Size: 6724 KpnI G_GTAC'C Cuts at: 4049 4049 Size: 6724 MfeI C'AATT_G Cuts at: 4323 4323 Size: 6724 MluI A'CGCG_T Cuts at: 6600 6600 Size: 6724 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2982 3450 3897 5838 6022 1305 Size: 1677 468 447 1941 184 2007 Fragments arranged by size: 2007 1941 1677 468 447 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4902 Fragments arranged by size: 4902 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3450 3500 4682 5041 5200 518 Size: 648 2284 50 1182 359 159 2042 Fragments arranged by size: 2284 2042 1182 648 359 159 50 MspA1I CmG'CkG Cuts at: 1606 2348 2401 2846 3232 3500 4140 4817 5965 Size: 742 53 445 386 268 640 677 1148 Cuts at: 5965 6210 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 742 677 640 445 386 268 245 53 NaeI GCC'GGC Cuts at: 2224 2617 2224 Size: 393 6331 NcoI C'CATG_G Cuts at: 513 1065 2004 2232 2763 513 Size: 552 939 228 531 4474 Fragments arranged by size: 4474 939 552 531 228 NdeI CA'TA_TG Cuts at: 387 387 Size: 6724 NgoMIV G'CCGG_C Cuts at: 2222 2615 2222 Size: 393 6331 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6724 NotI GC'GGCC_GC Cuts at: 4171 4171 Size: 6724 NspI r_CATG'y Cuts at: 1132 1229 3850 4082 5627 1132 Size: 97 2621 232 1545 2229 Fragments arranged by size: 2621 2229 1545 232 97 PasI CC'CwG_GG Cuts at: 1348 3625 1348 Size: 2277 4447 PciI A'CATG_T Cuts at: 3846 5623 3846 Size: 1777 4947 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6724 PfoI T'CCnGG_A Cuts at: 3012 3414 3012 Size: 402 6322 PmeI GTTT'AAAC Cuts at: 4028 4028 Size: 6724 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2437 2469 4793 4825 6306 6338 2437 Size: 32 2324 32 1481 32 2823 Fragments arranged by size: 2823 2324 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3418 3418 Size: 6724 PshAI GACnn'nnGTC Cuts at: 1613 2853 1613 Size: 1240 5484 PsiI TTA'TAA Cuts at: 4294 4294 Size: 6724 PstI C_TGCA'G Cuts at: 830 2388 2829 4076 830 Size: 1558 441 1247 3478 Fragments arranged by size: 3478 1558 1247 441 PvuI CG_AT'CG Cuts at: 2001 2241 2001 Size: 240 6484 PvuII CAG'CTG Cuts at: 2846 2846 Size: 6724 SacI G_AGCT'C Cuts at: 2124 2522 2699 3239 2124 Size: 398 177 540 5609 Fragments arranged by size: 5609 540 398 177 SacII CC_GC'GG Cuts at: 2349 2349 Size: 6724 SalI G'TCGA_C Cuts at: 1936 4066 1936 Size: 2130 4594 SapI GCTCTTCn'nnn_ Cuts at: 2723 2723 Size: 6724 SbfI CC_TGCA'GG Cuts at: 4076 4076 Size: 6724 ScaI AGT'ACT Cuts at: 4972 6684 4972 Size: 1712 5012 SfcI C'TryA_G Cuts at: 826 1045 2384 2825 3046 4072 5207 5888 6079 Size: 219 1339 441 221 1026 1135 681 191 Cuts at: 6079 826 Size: 1471 Fragments arranged by size: 1471 1339 1135 1026 681 441 221 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6724 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6724 SmaI CCC'GGG Cuts at: 1872 2300 2329 4051 1872 Size: 428 29 1722 4545 Fragments arranged by size: 4545 1722 428 29 SmlI C'TyrA_G Cuts at: 820 1017 1943 2285 3679 4828 5729 5991 820 Size: 197 926 342 1394 1149 901 262 1553 Fragments arranged by size: 1553 1394 1149 926 901 342 262 197 SnaBI TAC'GTA Cuts at: 493 4515 493 Size: 4022 2702 SpeI A'CTAG_T Cuts at: 152 3788 152 Size: 3636 3088 SphI G_CATG'C Cuts at: 1132 4082 1132 Size: 2950 3774 SspI AAT'ATT Cuts at: 5 52 4648 5 Size: 47 4596 2081 Fragments arranged by size: 4596 2081 47 StuI AGG'CCT Cuts at: 1517 3998 1517 Size: 2481 4243 StyI C'CwwG_G Cuts at: 513 1065 2004 2232 2763 3258 3697 4163 513 Size: 552 939 228 531 495 439 466 3074 Fragments arranged by size: 3074 939 552 531 495 466 439 228 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4003 4751 4768 4921 5106 5551 325 Size: 1255 2423 748 17 153 185 445 1498 Fragments arranged by size: 2423 1498 1255 748 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3780 4970 6682 Size: 274 80 33 51 1443 1803 1190 1712 Cuts at: 6682 96 Size: 138 Fragments arranged by size: 1803 1712 1443 1190 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6684 6716 6684 Size: 32 6692 Tth111I GACn'n_nGTC Cuts at: 1316 3466 1316 Size: 2150 4574 XbaI T'CTAG_A Cuts at: 4060 4060 Size: 6724 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3632 3632 Size: 6724 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6724 XmaI C'CCGG_G Cuts at: 1870 2298 2327 4049 1870 Size: 428 29 1722 4545 Fragments arranged by size: 4545 1722 428 29 XmnI GAAnn'nnTTC Cuts at: 4853 4853 Size: 6724 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4532 276 Size: 53 83 186 936 2998 2468 Fragments arranged by size: 2998 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SanDI SexAI SfiI SfoI SrfI SwaI