(Linear) (Six-base) MAPSORT of: KIBB7063.seq Check: 9452 from: 1 to: 2019 KIBB7063.orf With 182 enzymes: SgfI * July 15, 2010 18:31 .. AccI GT'mk_AC Cuts at: 0 778 1246 2019 Size: 778 468 773 Fragments arranged by size: 778 773 468 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1052 2019 Size: 1052 967 AflIII A'CryG_T Cuts at: 0 1841 2019 Size: 1841 178 AhdI GACnn_n'nnGTC Cuts at: 0 324 1872 2019 Size: 324 1548 147 Fragments arranged by size: 1548 324 147 AleI CACnn'nnGTG Cuts at: 0 1495 2019 Size: 1495 524 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 46 78 2019 Size: 46 32 1941 Fragments arranged by size: 1941 46 32 AlwNI CAG_nnn'CTG Cuts at: 0 375 1416 2019 Size: 375 1041 603 Fragments arranged by size: 1041 603 375 ApaLI G'TGCA_C Cuts at: 0 1083 2019 Size: 1083 936 ApoI r'AATT_y Cuts at: 0 1509 1653 2019 Size: 1509 144 366 Fragments arranged by size: 1509 366 144 AvaI C'yCGr_G Cuts at: 0 141 172 293 322 1260 2019 Size: 141 31 121 29 938 759 Fragments arranged by size: 938 759 141 121 31 29 BanI G'GyrC_C Cuts at: 0 9 303 1120 2010 2019 Size: 9 294 817 890 9 Fragments arranged by size: 890 817 294 9 9 BanII G_rGCy'C Cuts at: 0 71 119 130 268 430 517 694 1234 Size: 71 48 11 138 162 87 177 540 Cuts at: 1234 1261 1273 2019 Size: 27 12 746 Fragments arranged by size: 746 540 177 162 138 87 71 48 27 12 11 BbsI GAAGACnn'nnnn_ Cuts at: 0 197 2019 Size: 197 1822 BbvCI CC'TCA_GC Cuts at: 0 386 2019 Size: 386 1633 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1284 1318 2019 Size: 1284 34 701 Fragments arranged by size: 1284 701 34 BclI T'GATC_A Cuts at: 0 494 2019 Size: 494 1525 BglI GCCn_nnn'nGGC Cuts at: 0 343 2019 Size: 343 1676 BglII A'GATC_T Cuts at: 0 545 2019 Size: 545 1474 BlpI GC'TnA_GC Cuts at: 0 355 1050 1985 2019 Size: 355 695 935 34 Fragments arranged by size: 935 695 355 34 Bme1580I G_kGCm'C Cuts at: 0 575 1087 2019 Size: 575 512 932 Fragments arranged by size: 932 575 512 BmgBI CAC'GTC Cuts at: 0 887 2019 Size: 887 1132 BmrI ACTGGGnnnn_n' Cuts at: 0 1220 2019 Size: 1220 799 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1393 2019 Size: 1393 626 Bpu10I CC'TnA_GC Cuts at: 0 386 2019 Size: 386 1633 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 301 1695 2019 Size: 301 1394 324 Fragments arranged by size: 1394 324 301 BsaAI yAC'GTr Cuts at: 0 95 2019 Size: 95 1924 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 838 868 2019 Size: 838 30 1151 Fragments arranged by size: 1151 838 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 190 193 1758 2019 Size: 190 3 1565 261 Fragments arranged by size: 1565 261 190 3 BseYI C'CCAG_C Cuts at: 0 261 421 857 1196 1562 1822 2006 2019 Size: 261 160 436 339 366 260 184 13 Fragments arranged by size: 436 366 339 261 260 184 160 13 BsiEI CG_ry'CG Cuts at: 0 155 236 2019 Size: 155 81 1783 Fragments arranged by size: 1783 155 81 BsiHKAI G_wGCw'C Cuts at: 0 119 517 694 1087 1234 2019 Size: 119 398 177 393 147 785 Fragments arranged by size: 785 398 393 177 147 119 BsmBI CGTCTCn'nnnn_ Cuts at: 0 142 2019 Size: 142 1877 Bsp1286I G_dGCh'C Cuts at: 0 71 119 130 268 430 517 575 694 Size: 71 48 11 138 162 87 58 119 Cuts at: 694 1087 1234 1261 1273 2019 Size: 393 147 27 12 746 Fragments arranged by size: 746 393 162 147 138 119 87 71 58 48 27 12 11 BspHI T'CATG_A Cuts at: 0 1451 2019 Size: 1451 568 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1175 1424 1802 2019 Size: 1175 249 378 217 Fragments arranged by size: 1175 378 249 217 BsrBI CCG'CTC Cuts at: 0 30 39 254 359 728 2019 Size: 30 9 215 105 369 1291 Fragments arranged by size: 1291 369 215 105 30 9 BsrDI GCAATG_nn' Cuts at: 0 1542 2019 Size: 1542 477 BsrFI r'CCGG_y Cuts at: 0 12 217 610 2019 Size: 12 205 393 1409 Fragments arranged by size: 1409 393 205 12 BstAPI GCAn_nnn'nTGC Cuts at: 0 396 1082 2019 Size: 396 686 937 Fragments arranged by size: 937 686 396 BstEII G'GTnAC_C Cuts at: 0 1396 2019 Size: 1396 623 BstXI CCAn_nnnn'nTGG Cuts at: 0 1830 2019 Size: 1830 189 BstYI r'GATC_y Cuts at: 0 545 2019 Size: 545 1474 BstZ17I GTA'TAC Cuts at: 0 779 1247 2019 Size: 779 468 772 Fragments arranged by size: 779 772 468 BtgI C'CryG_G Cuts at: 0 227 341 758 789 1099 2019 Size: 227 114 417 31 310 920 Fragments arranged by size: 920 417 310 227 114 31 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1187 1460 2019 Size: 1187 273 559 Fragments arranged by size: 1187 559 273 BtsI GCAGTG_nn' Cuts at: 0 643 903 2019 Size: 643 260 1116 Fragments arranged by size: 1116 643 260 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 771 806 2019 Size: 771 35 1213 Fragments arranged by size: 1213 771 35 EaeI y'GGCC_r Cuts at: 0 152 792 963 2019 Size: 152 640 171 1056 Fragments arranged by size: 1056 640 171 152 EagI C'GGCC_G Cuts at: 0 152 2019 Size: 152 1867 EarI CTCTTCn'nnn_ Cuts at: 0 718 1383 2019 Size: 718 665 636 Fragments arranged by size: 718 665 636 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 474 2019 Size: 474 1545 EcoICRI GAG'CTC Cuts at: 0 117 515 692 1232 2019 Size: 117 398 177 540 787 Fragments arranged by size: 787 540 398 177 117 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1052 1393 2019 Size: 1052 341 626 Fragments arranged by size: 1052 626 341 EcoO109I rG'GnC_Cy Cuts at: 0 1413 2019 Size: 1413 606 EcoRI G'AATT_C Cuts at: 0 1509 1653 2019 Size: 1509 144 366 Fragments arranged by size: 1509 366 144 HaeII r_GCGC'y Cuts at: 0 376 1628 2019 Size: 376 1252 391 Fragments arranged by size: 1252 391 376 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 310 342 838 870 1744 1776 1858 1890 Size: 310 32 496 32 874 32 82 32 Cuts at: 1890 2019 Size: 129 Fragments arranged by size: 874 496 310 129 82 32 32 32 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 977 1445 1892 2019 Size: 977 468 447 127 Fragments arranged by size: 977 468 447 127 MslI CAynn'nnrTG Cuts at: 0 1445 1495 2019 Size: 1445 50 524 Fragments arranged by size: 1445 524 50 MspA1I CmG'CkG Cuts at: 0 343 396 841 1227 1495 2019 Size: 343 53 445 386 268 524 Fragments arranged by size: 524 445 386 343 268 53 NaeI GCC'GGC Cuts at: 0 219 612 2019 Size: 219 393 1407 Fragments arranged by size: 1407 393 219 NcoI C'CATG_G Cuts at: 0 227 758 2019 Size: 227 531 1261 Fragments arranged by size: 1261 531 227 NgoMIV G'CCGG_C Cuts at: 0 217 610 2019 Size: 217 393 1409 Fragments arranged by size: 1409 393 217 NspI r_CATG'y Cuts at: 0 1845 2019 Size: 1845 174 PasI CC'CwG_GG Cuts at: 0 1620 2019 Size: 1620 399 PciI A'CATG_T Cuts at: 0 1841 2019 Size: 1841 178 PfoI T'CCnGG_A Cuts at: 0 1007 1409 2019 Size: 1007 402 610 Fragments arranged by size: 1007 610 402 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 432 464 2019 Size: 432 32 1555 Fragments arranged by size: 1555 432 32 PpuMI rG'GwC_Cy Cuts at: 0 1413 2019 Size: 1413 606 PshAI GACnn'nnGTC Cuts at: 0 848 2019 Size: 848 1171 PstI C_TGCA'G Cuts at: 0 383 824 2019 Size: 383 441 1195 Fragments arranged by size: 1195 441 383 PvuI CG_AT'CG Cuts at: 0 236 2019 Size: 236 1783 PvuII CAG'CTG Cuts at: 0 841 2019 Size: 841 1178 SacI G_AGCT'C Cuts at: 0 119 517 694 1234 2019 Size: 119 398 177 540 785 Fragments arranged by size: 785 540 398 177 119 SacII CC_GC'GG Cuts at: 0 344 2019 Size: 344 1675 SapI GCTCTTCn'nnn_ Cuts at: 0 718 2019 Size: 718 1301 SfcI C'TryA_G Cuts at: 0 379 820 1041 2019 Size: 379 441 221 978 Fragments arranged by size: 978 441 379 221 SmaI CCC'GGG Cuts at: 0 295 324 2019 Size: 295 29 1695 Fragments arranged by size: 1695 295 29 SmlI C'TyrA_G Cuts at: 0 280 1674 2019 Size: 280 1394 345 Fragments arranged by size: 1394 345 280 SpeI A'CTAG_T Cuts at: 0 1783 2019 Size: 1783 236 StuI AGG'CCT Cuts at: 0 1993 2019 Size: 1993 26 StyI C'CwwG_G Cuts at: 0 227 758 1253 1692 2019 Size: 227 531 495 439 327 Fragments arranged by size: 531 495 439 327 227 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1998 2019 Size: 1998 21 TatI w'GTAC_w Cuts at: 0 1775 2019 Size: 1775 244 Tth111I GACn'n_nGTC Cuts at: 0 1461 2019 Size: 1461 558 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1627 2019 Size: 1627 392 XmaI C'CCGG_G Cuts at: 0 293 322 2019 Size: 293 29 1697 Fragments arranged by size: 1697 293 29 Enzymes that do cut: AccI AcuI AflIII AhdI AleI AloI AlwNI ApaLI ApoI AvaI BanI BanII BbsI BbvCI BcgI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaAI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BstAPI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI CspCI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI HaeII Hin4I MmeI MslI MspA1I NaeI NcoI NgoMIV NspI PasI PciI PfoI PpiI PpuMI PshAI PstI PvuI PvuII SacI SacII SapI SfcI SmaI SmlI SpeI StuI StyI TaqII TatI Tth111I XcmI XmaI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AgeI AlfI ApaI AscI AseI AsiSI AvrII BaeI BamHI BbeI BciVI BfrBI BmtI BplI BsaI BsaBI BsaHI BsaWI BsgI BsiWI BsmI BspEI BsrGI BssHII BssSI BstBI Bsu36I ClaI DraI DraIII DrdI EcoNI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI MscI NarI NdeI NheI NotI NruI NsiI PacI PflMI PmeI PmlI PsiI PspOMI PspXI PsrI RsrII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SphI SrfI SspI SwaI TstI XbaI XhoI XmnI ZraI