Restriction map for
pFN21AE5172
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# NO CUT ENZYMES ( 178 enzymes) #
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AatII Acc65I AccI AccII AccIII
AcsI AfaI AflII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbvCI BciVI
BfaI BfrI BglI BglII BlnI
BlpI BmyI Bpu1102I BsaAI BsaBI
BsaWI BseAI BsgI BsiHKAI BsiWI
BsmBI BsmFI BsmI Bsp1286I Bsp1407I
BspEI BspHI BspMI BsrDI BsrFI
BsrGI BsrI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I Bsu36I Cac8I
CelII CfoI Cfr10I CpoI DdeI
DraI DraII DraIII DrdI Eam1105I
EarI Eco47III Eco81I EcoO109I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FseI
FspI HaeII HhaI HinP1I HincII
HindII HindIII HpaI KasI KpnI
Ksp632I KspI MaeI MamI MfeI
MflI MluI MluNI MroI MscI
MseI MslI MspA1I MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI PstI PvuI PvuII RcaI
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfaNI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI StuI StyI SwaI Tru9I
Tth111I Van91I XbaI XcmI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AclI 413 AACGTT AA^CGTT
AcyI 58 GACGCC G[GA]^CG[CT]C
AflIII 425 ACATGT A^C[GA][CT]GT
AlwNI 46 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaI 85 CTCGAG C^[CT]CG[GA]G
AvaII 67 GGACC G^G[AT]CC
BbsI 419 GTCTTC GAAGAC(2/6)
BbvI 228 GCTGC GCAGC(8/12)
BcgI 77 GCAACTTCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 32 TGATCA T^GATCA
BpmI 364 CTGGAG CTGGAG(16/14)
BpuAI 419 GTCTTC GAAGAC(2/6)
BsaHI 58 GACGCC G[GA]^CG[CT]C
BsaI 7 GAGACC GGTCTC(1/5)
BseRI 311 GAGGAG GAGGAG(10/8)
BsiEI 123 CGGCCG CG[GA][CT]^CG
BsmAI 7 GAGAC GTCTC(1/5)
BsoBI 85 CTCGAG C^[CT]CG[GA]G
BspDI 91 ATCGAT AT^CGAT
BspLU11I 425 ACATGT A^CATGT
BsrBI 271 CCGCTC CCGCTC(-3/-3)
BstF5I 394 CATCC GGATG(2/0)
BstNI 277 CCTGG CC^[AT]GG
ClaI 91 ATCGAT AT^CGAT
DsaI 11 CCGTGG C^C[GA][CT]GG
EaeI 123 CGGCCG [CT]^GGCC[GA]
EagI 123 CGGCCG C^GGCCG
EclXI 123 CGGCCG C^GGCCG
Eco52I 123 CGGCCG C^GGCCG
EcoNI 304 CCTTTTAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII 277 CCTGG ^CC[AT]GG
FbaI 32 TGATCA T^GATCA
FokI 394 CATCC GGATG(9/13)
Hin1I 58 GACGCC G[GA]^CG[CT]C
HinfI 190 GAATC G^A[ACGT]TC
MaeII 414 ACGT A^CGT
MaeIII 215 GTGAC ^GT[ACGT]AC
MvaI 277 CCTGG CC^[AT]GG
MwoI 125 GCCGGGGCCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PaeR7I 85 CTCGAG C^TCGAG
Psp1406I 413 AACGTT AA^CGTT
SmlI 85 CTCGAG C^T[CT][GA]AG
SspI 324 AATATT AAT^ATT
TfiI 190 GAATC G^A[AT]TC
TseI 228 GCTGC G^C[AT]GC
Tsp45I 215 GTGAC ^GT[GC]AC
TspRI 213 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoI 85 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 21 enzymes) #
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BcnI 23 CCCGG CC^[GC]GG
126 CCGGG
Cfr13I 67 GGACC G^G[ACGT]CC
129 GGGCC
DpnII 33 GATC ^GATC
90 GATC
FauI 105 CCCGC CCCGC(4/6)
270 CCCGC
HaeIII 124 GGCC GG^CC
130 GGCC
HapII 24 CCGG C^CGG
126 CCGG
HgaI 58 GACGC GACGC(5/10)
294 GCGTC
HpaII 24 CCGG C^CGG
126 CCGG
HphI 35 TCACC GGTGA(8/7)
137 TCACC
MboI 33 GATC ^GATC
90 GATC
MspI 24 CCGG C^CGG
126 CCGG
NciI 23 CCCGG CC^[GC]GG
126 CCGGG
NdeII 33 GATC ^GATC
90 GATC
NlaIII 426 CATG CATG^
481 CATG
NlaIV 67 GGACCC GG[ACGT]^[ACGT]CC
128 GGGGCC
NspI 425 ACATGT [GA]CATG^[CT]
480 ACATGC
Sau3AI 33 GATC ^GATC
90 GATC
Sau96I 67 GGACC G^G[ACGT]CC
129 GGGCC
TaqI 86 TCGA T^CGA
92 TCGA
Tsp509I 239 AATT ^AATT
337 AATT
TthHB8I 86 TCGA T^CGA
92 TCGA
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# 3 OR MORE CUT ENZYMES ( 11 enzymes) #
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AciI AluI BsaJI BsiYI BslI
BssKI Fnu4HI ItaI MboII MnlI
ScrFI