(Circular) (Six-base) MAPSORT of: pFN21AE5172.seq Check: 1022 from: 1 to: 5191 pFN21AE5172 5191 bp With 182 enzymes: SgfI * January 30, 2014 17:14 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3001 278 Size: 53 83 186 936 1465 2468 Fragments arranged by size: 2468 1465 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2534 1937 Size: 597 4594 Acc65I G'GTAC_C Cuts at: 2512 2512 Size: 5191 AclI AA'CG_TT Cuts at: 1563 2419 3318 3691 1563 Size: 856 899 373 3063 Fragments arranged by size: 3063 899 856 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3254 4638 4906 1632 Size: 1622 1384 268 1917 Fragments arranged by size: 1917 1622 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4994 AflIII A'CryG_T Cuts at: 1203 2430 4090 5067 1203 Size: 1227 1660 977 1327 Fragments arranged by size: 1660 1327 1227 977 AgeI A'CCGG_T Cuts at: 4022 4022 Size: 5191 AhdI GACnn_n'nnGTC Cuts at: 3920 3920 Size: 5191 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5191 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5191 AlwNI CAG_nnn'CTG Cuts at: 2056 2934 4506 2056 Size: 878 1572 2741 Fragments arranged by size: 2741 1572 878 ApaLI G'TGCA_C Cuts at: 3248 4404 3248 Size: 1156 4035 ApoI r'AATT_y Cuts at: 1493 2500 2726 2878 4987 1493 Size: 1007 226 152 2109 1697 Fragments arranged by size: 2109 1697 1007 226 152 AseI AT'TA_AT Cuts at: 160 1032 3745 160 Size: 872 2713 1606 Fragments arranged by size: 2713 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5191 AvaI C'yCGr_G Cuts at: 1870 1943 2090 2516 1870 Size: 73 147 426 4545 Fragments arranged by size: 4545 426 147 73 BamHI G'GATC_C Cuts at: 2521 2888 2521 Size: 367 4824 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2512 3967 618 Size: 325 217 627 725 1455 1842 Fragments arranged by size: 1842 1455 725 627 325 217 BanII G_rGCy'C Cuts at: 1391 2510 1391 Size: 1119 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2417 928 Size: 971 518 3702 Fragments arranged by size: 3702 971 518 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2071 2105 3382 3416 1903 Size: 34 134 34 1277 34 3678 Fragments arranged by size: 3678 1277 134 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3083 4293 3083 Size: 1210 3981 BclI T'GATC_A Cuts at: 1548 2037 1548 Size: 489 4702 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3802 136 Size: 107 122 71 631 605 2130 1525 Fragments arranged by size: 2130 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5186 5186 Size: 5191 BlpI GC'TnA_GC Cuts at: 2608 2608 Size: 5191 Bme1580I G_kGCm'C Cuts at: 1790 3252 4408 1790 Size: 1462 1156 2573 Fragments arranged by size: 2573 1462 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5191 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2922 3880 442 Size: 416 53 83 399 24 1505 958 1753 Fragments arranged by size: 1753 1505 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5191 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2390 3851 1239 Size: 1151 1461 2579 Fragments arranged by size: 2579 1461 1151 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3316 4181 4479 3316 Size: 865 298 4028 Fragments arranged by size: 4028 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2006 3854 882 Size: 617 507 1848 2219 Fragments arranged by size: 2219 1848 617 507 BsaAI yAC'GTr Cuts at: 493 1204 2982 493 Size: 711 1778 2702 Fragments arranged by size: 2702 1778 711 BsaBI GATnn'nnATC Cuts at: 5185 5185 Size: 5191 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2064 2998 3380 4015 Size: 53 83 186 936 531 934 382 635 Cuts at: 4015 275 Size: 1451 Fragments arranged by size: 1451 936 934 635 531 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3624 4022 4296 4443 4854 3624 Size: 398 274 147 411 3961 Fragments arranged by size: 3961 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4966 Fragments arranged by size: 4966 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2331 1186 Size: 1145 4046 BseYI C'CCAG_C Cuts at: 2078 4394 4890 2078 Size: 2316 496 2379 Fragments arranged by size: 2379 2316 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4618 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2131 2641 3402 4430 1540 Size: 275 186 130 510 761 1028 2301 Fragments arranged by size: 2301 1028 761 510 275 186 130 BsiHKAI G_wGCw'C Cuts at: 3252 3337 4408 3252 Size: 85 1071 4035 Fragments arranged by size: 4035 1071 85 BsmI GAATG_Cn' Cuts at: 2702 2795 2702 Size: 93 5098 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2510 3252 3337 4408 1391 Size: 399 720 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 720 399 85 BspEI T'CCGG_A Cuts at: 4854 4854 Size: 5191 BspHI T'CATG_A Cuts at: 3080 4810 3080 Size: 1730 3461 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2546 844 Size: 1702 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2278 3078 1394 Size: 429 455 800 3507 Fragments arranged by size: 3507 800 455 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3686 3860 65 Size: 1183 2438 174 1396 Fragments arranged by size: 2438 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3835 4022 1700 Size: 110 2025 187 2869 Fragments arranged by size: 2869 2025 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5191 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5191 BssSI C'ACGA_G Cuts at: 3251 4263 3251 Size: 1012 4179 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5191 BstBI TT'CG_AA Cuts at: 3995 3995 Size: 5191 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5191 BstYI r'GATC_y Cuts at: 1972 2521 2888 3273 3290 4742 4828 4840 5186 Size: 549 367 385 17 1452 86 12 346 Cuts at: 5186 1972 Size: 1977 Fragments arranged by size: 1977 1452 549 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2016 513 Size: 552 939 12 3688 Fragments arranged by size: 3688 939 552 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4452 Fragments arranged by size: 4452 683 56 BtsI GCAGTG_nn' Cuts at: 2707 3501 3521 2707 Size: 794 20 4377 Fragments arranged by size: 4377 794 20 ClaI AT'CG_AT Cuts at: 1554 2097 1554 Size: 543 4648 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5156 DraI TTT'AAA Cuts at: 2495 2495 Size: 5191 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4198 809 Size: 507 2882 1802 Fragments arranged by size: 2882 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2128 2638 3527 Size: 54 1425 50 275 18 298 510 889 Cuts at: 3527 5156 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 510 298 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2128 2638 1537 Size: 275 316 510 4090 Fragments arranged by size: 4090 510 316 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3121 1353 Size: 1768 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 3778 4152 4298 387 Size: 1064 138 435 1754 374 146 1280 Fragments arranged by size: 1754 1280 1064 435 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2390 3254 3851 4638 4906 1239 Size: 393 758 864 597 787 268 1524 Fragments arranged by size: 1524 864 787 758 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2313 2313 Size: 5191 EcoRI G'AATT_C Cuts at: 2500 2878 2500 Size: 378 4813 FspI TGC'GCA Cuts at: 3697 3697 Size: 5191 HaeII r_GCGC'y Cuts at: 1678 4338 1678 Size: 2660 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3832 3864 3906 3938 1373 Size: 32 135 32 2260 32 42 32 2626 Fragments arranged by size: 2626 2260 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2535 2781 669 Size: 988 281 597 246 3079 Fragments arranged by size: 3079 988 597 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5191 HpaI GTT'AAC Cuts at: 2781 2781 Size: 5191 KpnI G_GTAC'C Cuts at: 2516 2516 Size: 5191 MfeI C'AATT_G Cuts at: 2790 2790 Size: 5191 MluI A'CGCG_T Cuts at: 5067 5067 Size: 5191 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4305 4489 1305 Size: 3000 184 2007 Fragments arranged by size: 3000 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3369 Fragments arranged by size: 3369 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3149 3508 3667 518 Size: 648 1983 359 159 2042 Fragments arranged by size: 2042 1983 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2607 3284 4432 4677 1606 Size: 1001 677 1148 245 2120 Fragments arranged by size: 2120 1148 1001 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3700 Fragments arranged by size: 3700 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5191 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5191 NotI GC'GGCC_GC Cuts at: 2638 2638 Size: 5191 NspI r_CATG'y Cuts at: 1132 1229 2434 2489 2549 4094 1132 Size: 97 1205 55 60 1545 2229 Fragments arranged by size: 2229 1545 1205 97 60 55 PasI CC'CwG_GG Cuts at: 1348 2282 1348 Size: 934 4257 PciI A'CATG_T Cuts at: 2430 4090 2430 Size: 1660 3531 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5191 PmeI GTTT'AAAC Cuts at: 2495 2495 Size: 5191 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3260 3292 4773 4805 3260 Size: 32 1481 32 3646 Fragments arranged by size: 3646 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5191 PsiI TTA'TAA Cuts at: 2381 2761 2381 Size: 380 4811 PstI C_TGCA'G Cuts at: 830 2543 830 Size: 1713 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5191 SalI G'TCGA_C Cuts at: 1936 2533 1936 Size: 597 4594 SbfI CC_TGCA'GG Cuts at: 2543 2543 Size: 5191 ScaI AGT'ACT Cuts at: 3439 5151 3439 Size: 1712 3479 SfcI C'TryA_G Cuts at: 826 1045 2539 3674 4355 4546 826 Size: 219 1494 1135 681 191 1471 Fragments arranged by size: 1494 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5191 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5191 SmaI CCC'GGG Cuts at: 1872 2518 1872 Size: 646 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2090 3295 4196 4458 820 Size: 197 926 147 1205 901 262 1553 Fragments arranged by size: 1553 1205 926 901 262 197 147 SnaBI TAC'GTA Cuts at: 493 2982 493 Size: 2489 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5191 SphI G_CATG'C Cuts at: 1132 2549 1132 Size: 1417 3774 SspI AAT'ATT Cuts at: 5 52 2331 3115 5 Size: 47 2279 784 2081 Fragments arranged by size: 2279 2081 784 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5191 StyI C'CwwG_G Cuts at: 513 1065 2004 2630 513 Size: 552 939 626 3074 Fragments arranged by size: 3074 939 626 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3218 3235 3388 3573 4018 325 Size: 1255 1638 17 153 185 445 1498 Fragments arranged by size: 1638 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3437 5149 96 Size: 274 80 33 51 1443 1460 1712 138 Fragments arranged by size: 1712 1460 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5151 5183 5151 Size: 32 5159 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5191 XbaI T'CTAG_A Cuts at: 2527 2527 Size: 5191 XhoI C'TCGA_G Cuts at: 1943 2090 1943 Size: 147 5044 XmaI C'CCGG_G Cuts at: 1870 2516 1870 Size: 646 4545 XmnI GAAnn'nnTTC Cuts at: 3320 3320 Size: 5191 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2999 276 Size: 53 83 186 936 1465 2468 Fragments arranged by size: 2468 1465 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI