################################## # NO CUT ENZYMES ( 145 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AflIII AgeI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspI AvaI AvrII BalI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsoBI BspDI BspEI BspHI BspLU11I BsrBI BsrFI BssSI Bst1107I BstBI BstEII BstF5I BstPI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EaeI EagI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT14I FbaI FokI FseI HgaI Hin1I HincII HindII HindIII HpaI HphI KasI KpnI MaeI MfeI MluI MluNI MroI MscI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SalI SapI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI StuI StyI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### ApaI 9 GGGCCC GGGCC^C AspHI 256 GAGCTC G[AT]GC[AT]^C AvaII 225 GGACC G^G[AT]CC AviII 308 TGCGCA TGC^GCA BamHI 240 GGATCC G^GATCC BpmI 157 CTGGAG CTGGAG(16/14) BsaBI 235 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiHKAI 256 GAGCTC G[AT]GC[AT]^C BsmBI 955 CGTCTC CGTCTC(1/5) Bsp1407I 701 TGTACA T^GTACA BsrDI 781 GCAATG GCAATG(2/0) BsrGI 701 TGTACA T^GTACA BssHII 92 GCGCGC G^CGCGC DraII 204 GGGGCCT [GA]G^G[ACGT]CC[CT] EarI 352 CTCTTC CTCTTC(1/4) EcoNI 183 CCTCCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 204 GGGGCCT [GA]G^G[ACGT]CC[CT] EcoT22I 762 ATGCAT ATGCA^T FauI 397 GCGGG CCCGC(4/6) FspI 308 TGCGCA TGC^GCA HaeII 313 AGCGCC [GA]GCGC^[CT] Ksp632I 352 CTCTTC CTCTTC(1/4) KspI 395 CCGCGG CCGC^GG MaeII 954 ACGT A^CGT MaeIII 839 GTGAC ^GT[ACGT]AC MamI 235 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NlaIII 494 CATG CATG^ NsiI 762 ATGCAT ATGCA^T PstI 187 CTGCAG CTGCA^G SacI 256 GAGCTC GAGCT^C SacII 395 CCGCGG CCGC^GG SbfI 186 CCTGCAGG CCTGCA^GG SfaNI 778 GATGC GCATC(5/9) SfcI 187 CTGCAG C^T[GA][CT]AG Sse8387I 186 CCTGCAGG CCTGCA^GG SspBI 701 TGTACA T^GTACA SspI 982 AATATT AAT^ATT TfiI 468 GATTC G^A[AT]TC Tsp45I 839 GTGAC ^GT[GC]AC ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AcsI 478 GAATTT [GA]^AATT[CT] 993 AAATTC AfaI 702 GTAC GT^AC 968 GTAC AhdI 133 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 294 GACAGGGTGTC AlwNI 151 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG 845 CAGTGCCTG ApoI 478 GAATTT [GA]^AATT[CT] 993 AAATTC AspEI 133 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 294 GACAGGGTGTC BanII 9 GGGCCC G[GA]GC[CT]^C 256 GAGCTC BbsI 124 GTCTTC GAAGAC(2/6) 892 GTCTTC BglI 42 GCCGCCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 54 GCCGCTGGGGC BmyI 9 GGGCCC G[AGT]GC[ACT]^C 256 GAGCTC BpuAI 124 GTCTTC GAAGAC(2/6) 892 GTCTTC BsmAI 434 GTCTC GTCTC(1/5) 956 GTCTC BsmI 452 GAATGC GAATGC(1/-1) 618 GAATGC Bsp1286I 9 GGGCCC G[AGT]GC[ACT]^C 256 GAGCTC BsrI 965 CCAGT ACTGG(1/-1) 971 CCAGT BstNI 87 CCTGG CC^[AT]GG 748 CCTGG BstYI 234 GGATCT [GA]^GATC[CT] 240 GGATCC Cac8I 81 GCTGGC GC[ACGT]^[ACGT]GC 92 GCGCGC DpnII 235 GATC ^GATC 241 GATC DsaI 46 CCGTGG C^C[GA][CT]GG 395 CCGCGG Eam1105I 133 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 294 GACAGGGTGTC EcoRII 87 CCTGG ^CC[AT]GG 748 CCTGG MboI 235 GATC ^GATC 241 GATC MflI 234 GGATCT [GA]^GATC[CT] 240 GGATCC MslI 755 CACATTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 765 CATTGATGTG MvaI 87 CCTGG CC^[AT]GG 748 CCTGG NdeII 235 GATC ^GATC 241 GATC PleI 414 GAGTC GAGTC(4/5) 807 GAGTC RsaI 702 GTAC GT^AC 968 GTAC Sau3AI 235 GATC ^GATC 241 GATC SmlI 730 CTTGAG C^T[CT][GA]AG 958 CTCAAG TaqI 795 TCGA T^CGA 810 TCGA TthHB8I 795 TCGA T^CGA 810 TCGA XhoII 234 GGATCT [GA]^GATC[CT] 240 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 40 enzymes) # ######################################### AccII AciI AluI AlwI BbvI BcnI BsaJI BsiYI BslI BsmFI BspMI BssKI BstAPI BstUI CfoI Cfr13I DdeI Fnu4HI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MboII MnlI MseI MspA1I MspI MvnI MwoI NciI NlaIV Sau96I ScrFI Tru9I TseI Tsp509I TspRI