Restriction map for
pFN21AE1850
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# NO CUT ENZYMES ( 145 enzymes) #
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AatII AccIII AclI AcsI AcyI
AflII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I AspEI AspI AvaI
AviII AvrII BalI BamHI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI BsoBI BspDI BspEI
BspHI BsrBI BsrDI BsrFI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstYI BstZ17I CelII Cfr10I ClaI
CpoI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HaeII Hin1I
HindIII KasI KspI MamI MfeI
MflI MluI MluNI MroI MscI
MunI NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI Tsp45I Tth111I Van91I XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Acc65I 233 GGTACC G^GTACC
AccI 274 GTCGAC GT^[AC][GT]AC
AccII 83 CGCG CG^CG
AflIII 678 ACATGT A^C[GA][CT]GT
AlwI 571 GATCC GGATC(4/5)
AlwNI 1016 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 366 CCTGAGG CC^T[ACGT]AGG
Asp718I 233 GGTACC G^GTACC
AspHI 1054 GAGCTC G[AT]GC[AT]^C
AvaII 1002 GGTCC G^G[AT]CC
BpmI 59 CTGGAG CTGGAG(16/14)
BseRI 24 CTCCTC GAGGAG(10/8)
BsgI 1071 CTGCAC GTGCAG(16/14)
BsiHKAI 1054 GAGCTC G[AT]GC[AT]^C
Bsp1407I 251 TGTACA T^GTACA
BspLU11I 678 ACATGT A^CATGT
BspMI 811 GCAGGT ACCTGC(4/8)
BsrGI 251 TGTACA T^GTACA
BstAPI 979 GCAGCTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 83 CGCG CG^CG
BstXI 120 CCAGCTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 366 CCTGAGG CC^T[ACGT]AGG
Cac8I 1069 GCCTGC GC[ACGT]^[ACGT]GC
CfoI 82 GCGC GCG^C
DraI 1035 TTTAAA TTT^AAA
DraII 54 GGGGCCT [GA]G^G[ACGT]CC[CT]
EaeI 9 TGGCCG [CT]^GGCC[GA]
Eco81I 366 CCTGAGG CC^T[ACGT]AGG
EcoO109I 54 GGGGCCT [GA]G^G[ACGT]CC[CT]
HgaI 84 GCGTC GACGC(5/10)
HhaI 82 GCGC GCG^C
HinP1I 82 GCGC G^CGC
HpaI 477 GTTAAC GTT^AAC
KpnI 233 GGTACC GGTAC^C
MaeII 455 ACGT A^CGT
MaeIII 941 GTTAC ^GT[ACGT]AC
MslI 949 CATTGGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 83 CGCG CG^CG
NdeI 174 CATATG CA^TATG
NspI 678 ACATGT [GA]CATG^[CT]
PleI 132 GACTC GAGTC(4/5)
PstI 635 CTGCAG CTGCA^G
PvuII 1082 CAGCTG CAG^CTG
SacI 1054 GAGCTC GAGCT^C
SalI 274 GTCGAC G^TCGAC
SapI 1051 GAAGAGC GCTCTTC(1/4)
ScaI 256 AGTACT AGT^ACT
SfaNI 649 GATGC GCATC(5/9)
SspBI 251 TGTACA T^GTACA
XbaI 690 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 19 enzymes) #
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BanI 233 GGTACC G^G[CT][GA]CC
372 GGTGCC
BanII 21 GGGCTC G[GA]GC[CT]^C
1054 GAGCTC
BcnI 29 CCGGG CC^[GC]GG
730 CCCGG
BmyI 21 GGGCTC G[AGT]GC[ACT]^C
1054 GAGCTC
BsmAI 246 GAGAC GTCTC(1/5)
631 GAGAC
BsmI 917 GAATGC GAATGC(1/-1)
875 GCATTC
Bsp1286I 21 GGGCTC G[AGT]GC[ACT]^C
1054 GAGCTC
BsrI 944 ACTGG ACTGG(1/-1)
574 CCAGT
EcoT14I 238 CCTTGG C^C[AT][AT]GG
782 CCTTGG
HapII 29 CCGG C^CGG
731 CCGG
HincII 274 GTCGAC GT[CT]^[GA]AC
477 GTTAAC
HindII 274 GTCGAC GT[CT]^[GA]AC
477 GTTAAC
HpaII 29 CCGG C^CGG
731 CCGG
MspA1I 279 CAGCGG C[AC]G^C[GT]G
1082 CAGCTG
MspI 29 CCGG C^CGG
731 CCGG
NciI 29 CCGGG CC^[GC]GG
730 CCCGG
StyI 238 CCTTGG C^C[AT][AT]GG
782 CCTTGG
TaqI 275 TCGA T^CGA
521 TCGA
TthHB8I 275 TCGA T^CGA
521 TCGA
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# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
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AciI AfaI AluI BbvI BfaI
BsaJI BsiYI BslI BsmFI BssKI
BstF5I BstNI Cfr13I DdeI DpnII
EarI EcoRII Fnu4HI FokI HaeIII
HinfI HphI ItaI Ksp632I MaeI
MboI MboII MnlI MseI MvaI
MwoI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfcI TfiI
Tru9I TseI Tsp509I TspRI