(Circular) (Six-base) MAPSORT of: pFN21AE1850.seq Check: 1066 from: 1 to: 5812 pFN21AE1850 5812 bp With 182 enzymes: SgfI * August 16, 2010 14:49 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3622 278 Size: 53 83 186 936 2086 2468 Fragments arranged by size: 2468 2086 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2280 3155 1937 Size: 343 875 4594 Fragments arranged by size: 4594 875 343 Acc65I G'GTAC_C Cuts at: 2238 3133 2238 Size: 895 4917 AclI AA'CG_TT Cuts at: 1563 3939 4312 1563 Size: 2376 373 3063 Fragments arranged by size: 3063 2376 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2496 2691 3125 3875 5259 5527 1632 Size: 864 195 434 750 1384 268 1917 Fragments arranged by size: 1917 1384 864 750 434 268 195 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5615 AflIII A'CryG_T Cuts at: 1203 2683 4711 5688 1203 Size: 1480 2028 977 1327 Fragments arranged by size: 2028 1480 1327 977 AgeI A'CCGG_T Cuts at: 4643 4643 Size: 5812 AhdI GACnn_n'nnGTC Cuts at: 4541 4541 Size: 5812 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5812 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5812 AlwNI CAG_nnn'CTG Cuts at: 3026 3555 5127 3026 Size: 529 1572 3711 Fragments arranged by size: 3711 1572 529 ApaLI G'TGCA_C Cuts at: 3869 5025 3869 Size: 1156 4656 ApoI r'AATT_y Cuts at: 1493 3121 3347 3499 5608 1493 Size: 1628 226 152 2109 1697 Fragments arranged by size: 2109 1697 1628 226 152 AseI AT'TA_AT Cuts at: 160 1032 4366 160 Size: 872 3334 1606 Fragments arranged by size: 3334 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5812 AvaI C'yCGr_G Cuts at: 1870 1943 3137 1870 Size: 73 1194 4545 Fragments arranged by size: 4545 1194 73 BamHI G'GATC_C Cuts at: 3142 3509 3142 Size: 367 5445 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2238 2377 3133 4588 618 Size: 325 217 627 451 139 756 1455 1842 Fragments arranged by size: 1842 1455 756 627 451 325 217 139 BanII G_rGCy'C Cuts at: 1391 2030 3063 3131 1391 Size: 639 1033 68 4072 Fragments arranged by size: 4072 1033 639 68 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4841 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4003 4037 1903 Size: 34 2066 34 3678 Fragments arranged by size: 3678 2066 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3704 4914 3704 Size: 1210 4602 BclI T'GATC_A Cuts at: 1548 1548 Size: 5812 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4423 136 Size: 107 122 71 631 605 2751 1525 Fragments arranged by size: 2751 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5807 5807 Size: 5812 BlpI GC'TnA_GC Cuts at: 3229 3229 Size: 5812 Bme1580I G_kGCm'C Cuts at: 1790 3873 5029 1790 Size: 2083 1156 2573 Fragments arranged by size: 2573 2083 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5812 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3543 4501 442 Size: 416 53 83 399 24 2126 958 1753 Fragments arranged by size: 2126 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5812 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2085 4472 1239 Size: 846 2387 2579 Fragments arranged by size: 2579 2387 846 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3937 4802 5100 3937 Size: 865 298 4649 Fragments arranged by size: 4649 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4475 882 Size: 617 2976 2219 Fragments arranged by size: 2976 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3603 493 Size: 711 2399 2702 Fragments arranged by size: 2702 2399 711 BsaBI GATnn'nnATC Cuts at: 5806 5806 Size: 5812 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3619 4001 4636 275 Size: 53 83 186 936 2086 382 635 1451 Fragments arranged by size: 2086 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4245 4643 4917 5064 5475 4245 Size: 398 274 147 411 4582 Fragments arranged by size: 4582 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2002 2032 1178 Size: 30 165 30 599 30 4958 Fragments arranged by size: 4958 599 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2020 1186 Size: 834 4978 BseYI C'CCAG_C Cuts at: 2023 2056 3019 5015 5511 2023 Size: 33 963 1996 496 2324 Fragments arranged by size: 2324 1996 963 496 33 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3061 1163 Size: 573 1325 3914 Fragments arranged by size: 3914 1325 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3262 4023 5051 1540 Size: 275 186 1261 761 1028 2301 Fragments arranged by size: 2301 1261 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3063 3873 3958 5029 3063 Size: 810 85 1071 3846 Fragments arranged by size: 3846 1071 810 85 BsmI GAATG_Cn' Cuts at: 2880 2928 3323 3416 2880 Size: 48 395 93 5276 Fragments arranged by size: 5276 395 93 48 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2030 3063 3131 3873 3958 5029 1391 Size: 399 240 1033 68 742 85 1071 2174 Fragments arranged by size: 2174 1071 1033 742 399 240 85 68 BspEI T'CCGG_A Cuts at: 5475 5475 Size: 5812 BspHI T'CATG_A Cuts at: 3701 5431 3701 Size: 1730 4082 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2807 3167 844 Size: 1963 360 3489 Fragments arranged by size: 3489 1963 360 BsrBI CCG'CTC Cuts at: 1394 1823 3699 1394 Size: 429 1876 3507 Fragments arranged by size: 3507 1876 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4307 4481 65 Size: 1183 3059 174 1396 Fragments arranged by size: 3059 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4456 4643 1700 Size: 110 2646 187 2869 Fragments arranged by size: 2869 2646 187 110 BsrGI T'GTAC_A Cuts at: 96 2256 96 Size: 2160 3652 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5812 BssSI C'ACGA_G Cuts at: 3872 4884 3872 Size: 1012 4800 BstAPI GCAn_nnn'nTGC Cuts at: 794 2990 794 Size: 2196 3616 BstBI TT'CG_AA Cuts at: 4616 4616 Size: 5812 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5812 BstXI CCAn_nnnn'nTGG Cuts at: 2132 2132 Size: 5812 BstYI r'GATC_y Cuts at: 1972 3142 3509 3894 3911 5363 5449 5461 5807 Size: 1170 367 385 17 1452 86 12 346 Cuts at: 5807 1972 Size: 1977 Fragments arranged by size: 1977 1452 1170 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2372 2372 Size: 5812 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5260 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5073 Fragments arranged by size: 5073 683 56 BtsI GCAGTG_nn' Cuts at: 3328 4122 4142 3328 Size: 794 20 4998 Fragments arranged by size: 4998 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5812 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5777 DraI TTT'AAA Cuts at: 3042 3116 3042 Size: 74 5738 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4819 809 Size: 507 3503 1802 Fragments arranged by size: 3503 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2014 3259 4148 Size: 54 1425 50 275 18 184 1245 889 Cuts at: 4148 5777 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1245 889 275 184 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3259 1537 Size: 275 1447 4090 Fragments arranged by size: 4090 1447 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2358 2616 2628 3051 3742 1353 Size: 1005 258 12 423 691 3423 Fragments arranged by size: 3423 1005 691 423 258 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4399 4773 4919 387 Size: 1064 138 2810 374 146 1280 Fragments arranged by size: 2810 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3061 3061 Size: 5812 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2085 2496 2691 3125 3875 4472 5259 Size: 393 453 411 195 434 750 597 787 Cuts at: 5259 5527 1239 Size: 268 1524 Fragments arranged by size: 1524 787 750 597 453 434 411 393 268 195 EcoO109I rG'GnC_Cy Cuts at: 2060 2060 Size: 5812 EcoRI G'AATT_C Cuts at: 3121 3499 3121 Size: 378 5434 FspI TGC'GCA Cuts at: 4318 4318 Size: 5812 HaeII r_GCGC'y Cuts at: 1678 4959 1678 Size: 3281 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4453 4485 4527 4559 1373 Size: 32 135 32 2881 32 42 32 2626 Fragments arranged by size: 2881 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2281 2484 3156 3402 669 Size: 988 281 343 203 672 246 3079 Fragments arranged by size: 3079 988 672 343 281 246 203 HindIII A'AGCT_T Cuts at: 748 748 Size: 5812 HpaI GTT'AAC Cuts at: 2484 3402 2484 Size: 918 4894 KpnI G_GTAC'C Cuts at: 2242 3137 2242 Size: 895 4917 MfeI C'AATT_G Cuts at: 3411 3411 Size: 5812 MluI A'CGCG_T Cuts at: 5688 5688 Size: 5812 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4926 5110 1305 Size: 3621 184 2007 Fragments arranged by size: 3621 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3990 Fragments arranged by size: 3990 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2958 3770 4129 4288 518 Size: 648 1792 812 359 159 2042 Fragments arranged by size: 2042 1792 812 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2286 3089 3228 3905 5053 5298 1606 Size: 680 803 139 677 1148 245 2120 Fragments arranged by size: 2120 1148 803 680 677 245 139 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5260 NdeI CA'TA_TG Cuts at: 387 2180 387 Size: 1793 4019 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5812 NotI GC'GGCC_GC Cuts at: 3259 3259 Size: 5812 NspI r_CATG'y Cuts at: 1132 1229 2687 3170 4715 1132 Size: 97 1458 483 1545 2229 Fragments arranged by size: 2229 1545 1458 483 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5812 PciI A'CATG_T Cuts at: 2683 4711 2683 Size: 2028 3784 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5812 PfoI T'CCnGG_A Cuts at: 2583 3009 2583 Size: 426 5386 PmeI GTTT'AAAC Cuts at: 3116 3116 Size: 5812 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3881 3913 5394 5426 3881 Size: 32 1481 32 4267 Fragments arranged by size: 4267 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5812 PsiI TTA'TAA Cuts at: 3382 3382 Size: 5812 PstI C_TGCA'G Cuts at: 830 2644 3164 830 Size: 1814 520 3478 Fragments arranged by size: 3478 1814 520 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5812 PvuII CAG'CTG Cuts at: 3089 3089 Size: 5812 SacI G_AGCT'C Cuts at: 3063 3063 Size: 5812 SalI G'TCGA_C Cuts at: 1936 2279 3154 1936 Size: 343 875 4594 Fragments arranged by size: 4594 875 343 SapI GCTCTTCn'nnn_ Cuts at: 3051 3051 Size: 5812 SbfI CC_TGCA'GG Cuts at: 3164 3164 Size: 5812 ScaI AGT'ACT Cuts at: 2263 4060 5772 2263 Size: 1797 1712 2303 Fragments arranged by size: 2303 1797 1712 SfcI C'TryA_G Cuts at: 826 1045 2141 2194 2640 3160 4295 4976 5167 Size: 219 1096 53 446 520 1135 681 191 Cuts at: 5167 826 Size: 1471 Fragments arranged by size: 1471 1135 1096 681 520 446 219 191 53 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5812 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5812 SmaI CCC'GGG Cuts at: 1872 3139 1872 Size: 1267 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3916 4817 5079 820 Size: 197 926 1973 901 262 1553 Fragments arranged by size: 1973 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3603 493 Size: 3110 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5812 SphI G_CATG'C Cuts at: 1132 3170 1132 Size: 2038 3774 SspI AAT'ATT Cuts at: 5 52 3736 5 Size: 47 3684 2081 Fragments arranged by size: 3684 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5812 StyI C'CwwG_G Cuts at: 513 1065 2243 2787 3251 513 Size: 552 1178 544 464 3074 Fragments arranged by size: 3074 1178 552 544 464 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3033 3839 3856 4009 4194 4639 325 Size: 1255 1453 806 17 153 185 445 1498 Fragments arranged by size: 1498 1453 1255 806 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2256 2261 2601 Size: 274 80 33 51 1443 279 5 340 Cuts at: 2601 4058 5770 96 Size: 1457 1712 138 Fragments arranged by size: 1712 1457 1443 340 279 274 138 80 51 33 5 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5772 5804 5772 Size: 32 5780 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5812 XbaI T'CTAG_A Cuts at: 2695 3148 2695 Size: 453 5359 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5812 XmaI C'CCGG_G Cuts at: 1870 3137 1870 Size: 1267 4545 XmnI GAAnn'nnTTC Cuts at: 3941 3941 Size: 5812 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3620 276 Size: 53 83 186 936 2086 2468 Fragments arranged by size: 2468 2086 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI