################################## # NO CUT ENZYMES ( 198 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AfaI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsiYI BslI BsmBI BsmFI BsoBI Bsp1407I BspDI BspEI BspHI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT14I EcoT22I FauI FbaI FokI FseI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HpaI HpaII HphI KasI KpnI KspI MaeII MamI MfeI MluI MluNI MroI MscI MslI MspA1I MspI MunI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SphI Sse8387I SspBI StuI StyI SwaI Tsp45I TspRI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AccII 19 CGCG CG^CG AflIII 256 ACATGT A^C[GA][CT]GT AlwI 220 GATCC GGATC(4/5) Asp700I 270 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 5 GAGCAC G[AT]GC[AT]^C BmyI 5 GAGCAC G[AGT]GC[ACT]^C BsiHKAI 5 GAGCAC G[AT]GC[AT]^C BsmAI 134 GTCTC GTCTC(1/5) BsmI 323 GCATTC GAATGC(1/-1) Bsp1286I 5 GAGCAC G[AGT]GC[ACT]^C BspLU11I 256 ACATGT A^CATGT BspMI 237 GCAGGT ACCTGC(4/8) BsrBI 50 GAGCGG CCGCTC(-3/-3) BssKI 395 CCTGG ^CC[ACGT]GG BstNI 395 CCTGG CC^[AT]GG BstUI 19 CGCG CG^CG BstXI 388 CCAGAAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 219 AGATCC [GA]^GATC[CT] DdeI 404 CTGAG C^T[ACGT]AG EarI 452 CTCTTC CTCTTC(1/4) EcoRII 395 CCTGG ^CC[AT]GG HindIII 379 AAGCTT A^AGCTT Ksp632I 452 CTCTTC CTCTTC(1/4) MaeIII 469 GTTAC ^GT[ACGT]AC MflI 219 AGATCC [GA]^GATC[CT] MvaI 395 CCTGG CC^[AT]GG MvnI 19 CGCG CG^CG NlaIII 257 CATG CATG^ NspI 256 ACATGT [GA]CATG^[CT] ScrFI 395 CCTGG CC^[ACGT]GG SpeI 9 ACTAGT A^CTAGT TaqI 482 TCGA T^CGA TthHB8I 482 TCGA T^CGA XhoII 219 AGATCC [GA]^GATC[CT] XmnI 270 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AciI 20 GCGG CCGC(-3/-1) 52 GCGG BbsI 499 GAAGAC GAAGAC(2/6) 228 GTCTTC BbvI 180 GCTGC GCAGC(8/12) 247 GCTGC BpuAI 499 GAAGAC GAAGAC(2/6) 228 GTCTTC DpnII 220 GATC ^GATC 518 GATC EcoRI 274 GAATTC G^AATTC 447 GAATTC Fnu4HI 180 GCTGC GC^[ACGT]GC 247 GCTGC HinfI 15 GATTC G^A[ACGT]TC 211 GATTC ItaI 180 GCTGC GC^[ACGT]GC 247 GCTGC MboI 220 GATC ^GATC 518 GATC NdeII 220 GATC ^GATC 518 GATC Sau3AI 220 GATC ^GATC 518 GATC SspI 313 AATATT AAT^ATT 461 AATATT TfiI 15 GATTC G^A[AT]TC 211 GATTC TseI 180 GCTGC G^C[AT]GC 247 GCTGC ######################################### # 3 OR MORE CUT ENZYMES ( 10 enzymes) # ######################################### AcsI AluI ApoI BfaI MaeI MboII MnlI MseI Tru9I Tsp509I