(Linear) (Six-base) MAPSORT of: KIEE6521.seq  Check: 4154  from: 1  to: 525



KIEE6521.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:33  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     518     525 
   Size:        518       7
 
AflIII A'CryG_T

Cuts at:      0     256     525 
   Size:        256     269
 
ApoI r'AATT_y

Cuts at:      0      30     274     338     447     525 
   Size:         30     244      64     109      78

  Fragments arranged by size: 

                 244     109      78      64      30
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     221     506     525 
   Size:        221     285      19

  Fragments arranged by size: 

                 285     221      19
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      26     525 
   Size:         26     499
 
BsiHKAI G_wGCw'C

Cuts at:      0       9     525 
   Size:          9     516
 
BsmI GAATG_Cn'

Cuts at:      0     323     525 
   Size:        323     202
 
Bsp1286I G_dGCh'C

Cuts at:      0       9     525 
   Size:          9     516
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     228     525 
   Size:        228     297
 
BsrBI CCG'CTC

Cuts at:      0      52     525 
   Size:         52     473
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     395     525 
   Size:        395     130
 
BstYI r'GATC_y

Cuts at:      0     219     525 
   Size:        219     306
 
EarI CTCTTCn'nnn_

Cuts at:      0     458     525 
   Size:        458      67
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     518     525 
   Size:        518       7
 
EcoRI G'AATT_C

Cuts at:      0     274     447     525 
   Size:        274     173      78

  Fragments arranged by size: 

                 274     173      78
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     211     243     525 
   Size:        211      32     282

  Fragments arranged by size: 

                 282     211      32
 
HindIII A'AGCT_T

Cuts at:      0     379     525 
   Size:        379     146
 
NspI r_CATG'y

Cuts at:      0     260     525 
   Size:        260     265
 
PciI A'CATG_T

Cuts at:      0     256     525 
   Size:        256     269
 
SpeI A'CTAG_T

Cuts at:      0       9     525 
   Size:          9     516
 
SspI AAT'ATT

Cuts at:      0     315     463     525 
   Size:        315     148      62

  Fragments arranged by size: 

                 315     148      62
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      28     525 
   Size:         28     497
 
XmnI GAAnn'nnTTC

Cuts at:      0     274     525 
   Size:        274     251
 

 Enzymes that do cut:

     AcuI   AflIII     ApoI     BbsI    BsaXI  BsiHKAI     BsmI Bsp1286I
    BspMI    BsrBI    BstXI    BstYI     EarI  Eco57MI    EcoRI    Hin4I
  HindIII     NspI     PciI     SpeI     SspI     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI    BseYI
     BsgI    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRV     FalI
     FseI     FspI    FspAI    HaeII   HincII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SphI     SrfI     StuI     StyI     SwaI    TaqII     TatI  Tth111I
     XbaI     XcmI     XhoI     XmaI     ZraI