################################## # NO CUT ENZYMES ( 130 enzymes) # ################################## AatII AccII AccIII AcsI AcyI AflII AflIII AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AviII AvrII BalI BamHI BbeI BbrPI BbsI BcgI BciVI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BseAI BsgI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrGI BssHII Bst1107I BstBI BstUI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoRI EcoRV EcoT22I FseI FspI HaeII Hin1I HincII HindII HpaI KasI KspI MamI MluI MluNI MroI MscI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PmaCI PmeI PmlI PpuMI PshBI PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 64 enzymes) # ################################### AccI 1105 GTCTAC GT^[AC][GT]AC AclI 1156 AACGTT AA^CGTT AgeI 1036 ACCGGT A^CCGGT AspHI 851 GTGCTC G[AT]GC[AT]^C AspI 526 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 755 CCCGGG C^[CT]CG[GA]G BanII 1029 GGGCTC G[GA]GC[CT]^C BbvCI 481 CCTCAGC CCTCAGC(-5/-2) BclI 951 TGATCA T^GATCA BfaI 629 CTAG C^TAG BpmI 838 CTGGAG CTGGAG(16/14) BsaWI 1036 ACCGGT [AT]^CCGG[AT] BsiHKAI 851 GTGCTC G[AT]GC[AT]^C BsmFI 806 GGGAC GGGAC(10/14) BsoBI 755 CCCGGG C^[CT]CG[GA]G BspHI 513 TCATGA T^CATGA BspMI 386 GCAGGT ACCTGC(4/8) BsrDI 1098 CATTGC GCAATG(2/0) BsrFI 1036 ACCGGT [GA]^CCGG[CT] BsrI 1119 CCAGT ACTGG(1/-1) BssSI 791 CACGAG CACGAG(-5/-1) BstAPI 188 GCATCGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 637 GGTGACC G^GT[ACGT]ACC BstF5I 1128 GGATG GGATG(2/0) BstPI 637 GGTGACC G^GT[ACGT]ACC Cac8I 849 GCGTGC GC[ACGT]^[ACGT]GC CfoI 1089 GCGC GCG^C Cfr10I 1036 ACCGGT [GA]^CCGG[CT] EaeI 708 TGGCCG [CT]^GGCC[GA] EarI 833 CTCTTC CTCTTC(1/4) EcoNI 281 CCTTAACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 637 GGTGACC G^GT[ACGT]ACC EcoT14I 815 CCTTGG C^C[AT][AT]GG FauI 142 CCCGC CCCGC(4/6) FbaI 951 TGATCA T^GATCA FokI 1128 GGATG GGATG(9/13) HhaI 1089 GCGC GCG^C HinP1I 1089 GCGC G^CGC HindIII 445 AAGCTT A^AGCTT Ksp632I 833 CTCTTC CTCTTC(1/4) MaeI 629 CTAG C^TAG MaeIII 638 GTGAC ^GT[ACGT]AC MfeI 1224 CAATTG C^AATTG MslI 435 CACAGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 437 CAGCTG C[AC]G^C[GT]G MunI 1224 CAATTG C^AATTG NspI 486 GCATGT [GA]CATG^[CT] PflFI 526 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1119 CCAGTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 1036 ACCGGT A^CCGGT PshAI 753 GACCCGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 1156 AACGTT AA^CGTT PstI 667 CTGCAG CTGCA^G PvuII 437 CAGCTG CAG^CTG RcaI 513 TCATGA T^CATGA SapI 832 GCTCTTC GCTCTTC(1/4) SmaI 755 CCCGGG CCC^GGG StuI 736 AGGCCT AGG^CCT StyI 815 CCTTGG C^C[AT][AT]GG Tsp45I 638 GTGAC ^GT[GC]AC Tth111I 526 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1119 CCAGTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 755 CCCGGG C^CCGGG XmaI 755 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### Acc65I 99 GGTACC G^GTACC 652 GGTACC AciI 143 CCGC CCGC(-3/-1) 1102 GCGG AfaI 100 GTAC GT^AC 653 GTAC Asp718I 99 GGTACC G^GTACC 652 GGTACC AvaII 44 GGACC G^G[AT]CC 752 GGACC BbvI 12 GCTGC GCAGC(8/12) 499 GCTGC BseRI 612 GAGGAG GAGGAG(10/8) 794 GAGGAG BsmAI 253 GAGAC GTCTC(1/5) 363 GAGAC BsmI 193 GAATGC GAATGC(1/-1) 467 GCATTC BstYI 58 GGATCT [GA]^GATC[CT] 1065 AGATCC Cfr13I 44 GGACC G^G[ACGT]CC 752 GGACC DsaI 452 CCACGG C^C[GA][CT]GG 1134 CCACGG Fnu4HI 12 GCTGC GC^[ACGT]GC 499 GCTGC HaeIII 709 GGCC GG^CC 737 GGCC HgaI 808 GACGC GACGC(5/10) 1056 GCGTC ItaI 12 GCTGC GC^[ACGT]GC 499 GCTGC KpnI 99 GGTACC GGTAC^C 652 GGTACC MaeII 597 ACGT A^CGT 1157 ACGT MflI 58 GGATCT [GA]^GATC[CT] 1065 AGATCC MseI 283 TTAA T^TAA 415 TTAA PleI 321 GACTC GAGTC(4/5) 1024 GAGTC RsaI 100 GTAC GT^AC 653 GTAC Sau96I 44 GGACC G^G[ACGT]CC 752 GGACC SfcI 459 CTGTAG C^T[GA][CT]AG 667 CTGCAG SmlI 90 CTTGAG C^T[CT][GA]AG 313 CTTGAG TaqI 191 TCGA T^CGA 600 TCGA Tru9I 283 TTAA T^TAA 415 TTAA TseI 12 GCTGC G^C[AT]GC 499 GCTGC TthHB8I 191 TCGA T^CGA 600 TCGA XhoII 58 GGATCT [GA]^GATC[CT] 1065 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 34 enzymes) # ######################################### AluI AlwI BanI BcnI BmyI BsaJI BsiYI BslI Bsp1286I BssKI BstNI DdeI DpnII EcoRII HapII HinfI HpaII HphI MboI MboII MnlI MspI MvaI MwoI NciI NdeII NlaIII NlaIV Sau3AI ScrFI SfaNI TfiI Tsp509I TspRI