(Circular) (Six-base) MAPSORT of: pFN21AE6395.seq Check: 6790 from: 1 to: 5944 pFN21AE6395 5944 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3754 278 Size: 53 83 186 936 2218 2468 Fragments arranged by size: 2468 2218 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3111 3287 1937 Size: 1174 176 4594 Fragments arranged by size: 4594 1174 176 Acc65I G'GTAC_C Cuts at: 2104 2657 3265 2104 Size: 553 608 4783 Fragments arranged by size: 4783 608 553 AclI AA'CG_TT Cuts at: 1563 3162 4071 4444 1563 Size: 1599 909 373 3063 Fragments arranged by size: 3063 1599 909 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2870 4007 5391 5659 1632 Size: 1238 1137 1384 268 1917 Fragments arranged by size: 1917 1384 1238 1137 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5747 AflIII A'CryG_T Cuts at: 1203 4843 5820 1203 Size: 3640 977 1327 Fragments arranged by size: 3640 1327 977 AgeI A'CCGG_T Cuts at: 3041 4775 3041 Size: 1734 4210 AhdI GACnn_n'nnGTC Cuts at: 4673 4673 Size: 5944 AleI CACnn'nnGTG Cuts at: 1166 2444 1166 Size: 1278 4666 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5944 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2399 2431 2399 Size: 32 5912 AlwNI CAG_nnn'CTG Cuts at: 3687 5259 3687 Size: 1572 4372 ApaLI G'TGCA_C Cuts at: 4001 5157 4001 Size: 1156 4788 ApoI r'AATT_y Cuts at: 1493 3253 3479 3631 5740 1493 Size: 1760 226 152 2109 1697 Fragments arranged by size: 2109 1760 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4498 160 Size: 872 3466 1606 Fragments arranged by size: 3466 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5944 AvaI C'yCGr_G Cuts at: 1870 1943 2760 3269 1870 Size: 73 817 509 4545 Fragments arranged by size: 4545 817 509 73 BamHI G'GATC_C Cuts at: 3274 3641 3274 Size: 367 5577 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2104 2276 2381 2657 3120 Size: 325 217 627 317 172 105 276 463 Cuts at: 3120 3265 4720 618 Size: 145 1455 1842 Fragments arranged by size: 1842 1455 627 463 325 317 276 217 172 145 105 BanII G_rGCy'C Cuts at: 1391 3038 3263 1391 Size: 1647 225 4072 Fragments arranged by size: 4072 1647 225 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4973 BbvCI CC'TCA_GC Cuts at: 2487 2487 Size: 5944 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4135 4169 1903 Size: 34 2198 34 3678 Fragments arranged by size: 3678 2198 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3836 5046 3836 Size: 1210 4734 BclI T'GATC_A Cuts at: 1548 2956 1548 Size: 1408 4536 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4555 136 Size: 107 122 71 631 605 2883 1525 Fragments arranged by size: 2883 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5939 5939 Size: 5944 BlpI GC'TnA_GC Cuts at: 3361 3361 Size: 5944 Bme1580I G_kGCm'C Cuts at: 1790 2797 3123 4005 5161 1790 Size: 1007 326 882 1156 2573 Fragments arranged by size: 2573 1156 1007 882 326 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5944 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3675 4633 442 Size: 416 53 83 399 24 2258 958 1753 Fragments arranged by size: 2258 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5944 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3052 3052 Size: 5944 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2864 4604 1239 Size: 1625 1740 2579 Fragments arranged by size: 2579 1740 1625 Bpu10I CC'TnA_GC Cuts at: 2487 2555 2487 Size: 68 5876 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2116 2339 4069 4934 5232 2116 Size: 223 1730 865 298 2828 Fragments arranged by size: 2828 1730 865 298 223 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4607 882 Size: 617 3108 2219 Fragments arranged by size: 3108 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3735 493 Size: 711 2531 2702 Fragments arranged by size: 2702 2531 711 BsaBI GATnn'nnATC Cuts at: 5938 5938 Size: 5944 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3751 4133 4768 275 Size: 53 83 186 936 2218 382 635 1451 Fragments arranged by size: 2218 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3041 4377 4775 5049 5196 5607 3041 Size: 1336 398 274 147 411 3378 Fragments arranged by size: 3378 1336 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2340 2370 1178 Size: 30 165 30 937 30 4752 Fragments arranged by size: 4752 937 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2632 2814 1186 Size: 1446 182 4316 Fragments arranged by size: 4316 1446 182 BseYI C'CCAG_C Cuts at: 2222 2790 5147 5643 2222 Size: 568 2357 496 2523 Fragments arranged by size: 2523 2357 568 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5371 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3394 4155 5183 1540 Size: 275 186 1393 761 1028 2301 Fragments arranged by size: 2301 1393 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2860 4005 4090 5161 2860 Size: 1145 85 1071 3643 Fragments arranged by size: 3643 1145 1071 85 BsmI GAATG_Cn' Cuts at: 2204 2472 3455 3548 2204 Size: 268 983 93 4600 Fragments arranged by size: 4600 983 268 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2797 2860 3038 3123 3263 4005 4090 Size: 399 1007 63 178 85 140 742 85 Cuts at: 4090 5161 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 1007 742 399 178 140 85 85 63 BspEI T'CCGG_A Cuts at: 5607 5607 Size: 5944 BspHI T'CATG_A Cuts at: 2518 3833 5563 2518 Size: 1315 1730 2899 Fragments arranged by size: 2899 1730 1315 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2382 3299 844 Size: 1538 917 3489 Fragments arranged by size: 3489 1538 917 BsrBI CCG'CTC Cuts at: 1394 1823 3831 1394 Size: 429 2008 3507 Fragments arranged by size: 3507 2008 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3102 4439 4613 65 Size: 1183 1854 1337 174 1396 Fragments arranged by size: 1854 1396 1337 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3041 4588 4775 1700 Size: 110 1231 1547 187 2869 Fragments arranged by size: 2869 1547 1231 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5944 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5944 BssSI C'ACGA_G Cuts at: 2796 4004 5016 2796 Size: 1208 1012 3724 Fragments arranged by size: 3724 1208 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2199 794 Size: 1405 4539 BstBI TT'CG_AA Cuts at: 4748 4748 Size: 5944 BstEII G'GTnAC_C Cuts at: 1184 2642 1184 Size: 1458 4486 BstYI r'GATC_y Cuts at: 1972 2063 3070 3274 3641 4026 4043 5495 5581 Size: 91 1007 204 367 385 17 1452 86 Cuts at: 5581 5593 5939 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1007 385 367 346 204 91 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2457 3139 513 Size: 552 939 453 682 3318 Fragments arranged by size: 3318 939 682 552 453 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3036 487 Size: 683 56 1810 3395 Fragments arranged by size: 3395 1810 683 56 BtsI GCAGTG_nn' Cuts at: 2986 3460 4254 4274 2986 Size: 474 794 20 4656 Fragments arranged by size: 4656 794 474 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5944 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5909 DraI TTT'AAA Cuts at: 3248 3248 Size: 5944 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4951 809 Size: 507 3635 1802 Fragments arranged by size: 3635 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2713 3391 4280 Size: 54 1425 50 275 18 883 678 889 Cuts at: 4280 5909 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 883 678 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3391 1537 Size: 275 1579 4090 Fragments arranged by size: 4090 1579 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2844 3874 1353 Size: 1491 1030 3423 Fragments arranged by size: 3423 1491 1030 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4531 4905 5051 387 Size: 1064 138 2942 374 146 1280 Fragments arranged by size: 2942 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2864 2870 4007 4604 5391 5659 1239 Size: 393 1232 6 1137 597 787 268 1524 Fragments arranged by size: 1524 1232 1137 787 597 393 268 6 EcoNI CCTnn'n_nnAGG Cuts at: 2290 2290 Size: 5944 EcoRI G'AATT_C Cuts at: 3253 3631 3253 Size: 378 5566 FspI TGC'GCA Cuts at: 4450 4450 Size: 5944 HaeII r_GCGC'y Cuts at: 1678 5091 1678 Size: 3413 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2119 2151 3014 3046 4585 Size: 32 135 32 547 32 863 32 1539 Cuts at: 4585 4617 4659 4691 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1539 863 547 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3288 3534 669 Size: 988 281 1350 246 3079 Fragments arranged by size: 3079 1350 988 281 246 HindIII A'AGCT_T Cuts at: 748 2450 748 Size: 1702 4242 HpaI GTT'AAC Cuts at: 3534 3534 Size: 5944 KpnI G_GTAC'C Cuts at: 2108 2661 3269 2108 Size: 553 608 4783 Fragments arranged by size: 4783 608 553 MfeI C'AATT_G Cuts at: 3229 3543 3229 Size: 314 5630 MluI A'CGCG_T Cuts at: 5820 5820 Size: 5944 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3012 5058 5242 1305 Size: 1707 2046 184 2007 Fragments arranged by size: 2046 2007 1707 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4122 Fragments arranged by size: 4122 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2444 3902 4261 4420 518 Size: 648 1278 1458 359 159 2042 Fragments arranged by size: 2042 1458 1278 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2444 3360 4037 5185 5430 1606 Size: 838 916 677 1148 245 2120 Fragments arranged by size: 2120 1148 916 838 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4453 Fragments arranged by size: 4453 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5944 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5944 NotI GC'GGCC_GC Cuts at: 3391 3391 Size: 5944 NspI r_CATG'y Cuts at: 1132 1229 2495 3302 4847 1132 Size: 97 1266 807 1545 2229 Fragments arranged by size: 2229 1545 1266 807 97 PasI CC'CwG_GG Cuts at: 1348 2042 1348 Size: 694 5250 PciI A'CATG_T Cuts at: 4843 4843 Size: 5944 PflMI CCAn_nnn'nTGG Cuts at: 1108 3130 1108 Size: 2022 3922 PmeI GTTT'AAAC Cuts at: 3248 3248 Size: 5944 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4013 4045 5526 5558 4013 Size: 32 1481 32 4399 Fragments arranged by size: 4399 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2762 1613 Size: 1149 4795 PsiI TTA'TAA Cuts at: 3514 3514 Size: 5944 PstI C_TGCA'G Cuts at: 830 2676 3296 830 Size: 1846 620 3478 Fragments arranged by size: 3478 1846 620 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5944 PvuII CAG'CTG Cuts at: 2444 2444 Size: 5944 SalI G'TCGA_C Cuts at: 1936 3286 1936 Size: 1350 4594 SapI GCTCTTCn'nnn_ Cuts at: 2844 2844 Size: 5944 SbfI CC_TGCA'GG Cuts at: 3296 3296 Size: 5944 ScaI AGT'ACT Cuts at: 4192 5904 4192 Size: 1712 4232 SfcI C'TryA_G Cuts at: 826 1045 2464 2672 3292 4427 5108 5299 826 Size: 219 1419 208 620 1135 681 191 1471 Fragments arranged by size: 1471 1419 1135 681 620 219 208 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5944 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5944 SmaI CCC'GGG Cuts at: 1872 2762 3271 1872 Size: 890 509 4545 Fragments arranged by size: 4545 890 509 SmlI C'TyrA_G Cuts at: 820 1017 1943 2095 2318 4048 4949 5211 820 Size: 197 926 152 223 1730 901 262 1553 Fragments arranged by size: 1730 1553 926 901 262 223 197 152 SnaBI TAC'GTA Cuts at: 493 3735 493 Size: 3242 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5944 SphI G_CATG'C Cuts at: 1132 3302 1132 Size: 2170 3774 SspI AAT'ATT Cuts at: 5 52 3868 5 Size: 47 3816 2081 Fragments arranged by size: 3816 2081 47 StuI AGG'CCT Cuts at: 1517 2743 1517 Size: 1226 4718 StyI C'CwwG_G Cuts at: 513 1065 2004 2820 3383 513 Size: 552 939 816 563 3074 Fragments arranged by size: 3074 939 816 563 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2034 3118 3971 3988 4141 4326 4771 Size: 1255 454 1084 853 17 153 185 445 Cuts at: 4771 325 Size: 1498 Fragments arranged by size: 1498 1255 1084 853 454 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4190 5902 96 Size: 274 80 33 51 1443 2213 1712 138 Fragments arranged by size: 2213 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2072 2104 2136 2572 2604 5904 5936 2072 Size: 32 32 436 32 3300 32 2080 Fragments arranged by size: 3300 2080 436 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2534 1316 Size: 1218 4726 XbaI T'CTAG_A Cuts at: 3280 3280 Size: 5944 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5944 XmaI C'CCGG_G Cuts at: 1870 2760 3269 1870 Size: 890 509 4545 Fragments arranged by size: 4545 890 509 XmnI GAAnn'nnTTC Cuts at: 4073 4073 Size: 5944 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3752 276 Size: 53 83 186 936 2218 2468 Fragments arranged by size: 2468 2218 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI