################################## # NO CUT ENZYMES ( 99 enzymes) # ################################## AatII Acc65I AclI AcsI AgeI AhdI AlwNI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BamHI BbrPI BcgI BciVI BclI BglII BlnI BlpI Bpu1102I BsaBI BsiWI BsmI Bsp1407I BspDI BspHI BspLU11I BsrGI BssSI Bst1107I BstAPI BstBI BstXI BstYI BstZ17I CelII ClaI CpoI DraI Eam1105I EcoRI EcoRV EcoT14I FbaI FseI HindIII KpnI MamI MfeI MflI MluI MunI NcoI NdeI NheI NotI NruI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SapI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI SspBI SspI StuI StyI SwaI Tth111I Van91I XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### AccI 848 GTCTAC GT^[AC][GT]AC AccIII 975 TCCGGA T^CCGGA AfaI 70 GTAC GT^AC AflII 720 CTTAAG C^TTAAG AflIII 752 ACGTGT A^C[GA][CT]GT Alw44I 620 GTGCAC G^TGCAC AocI 705 CCTCAGG CC^T[ACGT]AGG Aor51HI 63 AGCGCT AGC^GCT ApaI 21 GGGCCC GGGCC^C ApaLI 620 GTGCAC G^TGCAC AviII 278 TGCGCA TGC^GCA BalI 650 TGGCCA TGG^CCA BfrI 720 CTTAAG C^TTAAG BglI 604 GCCCTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 793 CTGGAG CTGGAG(16/14) BsaI 84 GGTCTC GGTCTC(1/5) BseAI 975 TCCGGA T^CCGGA BsgI 943 GTGCAG GTGCAG(16/14) BsiEI 340 CGGCCG CG[GA][CT]^CG BsmBI 504 CGTCTC CGTCTC(1/5) BspEI 975 TCCGGA T^CCGGA BsrBI 565 GAGCGG CCGCTC(-3/-3) BsrDI 494 GCAATG GCAATG(2/0) BstEII 915 GGTGACC G^GT[ACGT]ACC BstPI 915 GGTGACC G^GT[ACGT]ACC Bsu36I 705 CCTCAGG CC^T[ACGT]AGG DraIII 544 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 747 GACCTACGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 340 CGGCCG C^GGCCG EarI 286 CTCTTC CTCTTC(1/4) EclXI 340 CGGCCG C^GGCCG Eco47III 63 AGCGCT AGC^GCT Eco52I 340 CGGCCG C^GGCCG Eco81I 705 CCTCAGG CC^T[ACGT]AGG EcoNI 387 CCTGGACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 915 GGTGACC G^GT[ACGT]ACC EcoT22I 1153 ATGCAT ATGCA^T FspI 278 TGCGCA TGC^GCA HincII 232 GTTAAC GT[CT]^[GA]AC HindII 232 GTTAAC GT[CT]^[GA]AC HpaI 232 GTTAAC GTT^AAC Ksp632I 286 CTCTTC CTCTTC(1/4) KspI 676 CCGCGG CCGC^GG MluNI 650 TGGCCA TGG^CCA MroI 975 TCCGGA T^CCGGA MscI 650 TGGCCA TGG^CCA MslI 591 CATGGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NsiI 1153 ATGCAT ATGCA^T PvuII 304 CAGCTG CAG^CTG RsaI 70 GTAC GT^AC SacII 676 CCGCGG CCGC^GG SbfI 1172 CCTGCAGG CCTGCA^GG SmaI 17 CCCGGG CCC^GGG Sse8387I 1172 CCTGCAGG CCTGCA^GG Tsp509I 730 AATT ^AATT XbaI 777 TCTAGA T^CTAGA XcmI 515 CCAGTCCCCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 17 CCCGGG C^CCGGG XmaI 17 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AlwI 534 GATCC GGATC(4/5) 1179 GGATC AvaI 17 CCCGGG C^[CT]CG[GA]G 486 CTCGGG BanII 21 GGGCCC G[GA]GC[CT]^C 631 GGGCTC BbsI 93 GTCTTC GAAGAC(2/6) 1069 GTCTTC BbvCI 372 CCTCAGC CCTCAGC(-5/-2) 444 CCTCAGC BfaI 61 CTAG C^TAG 778 CTAG BpuAI 93 GTCTTC GAAGAC(2/6) 1069 GTCTTC BsaAI 274 TACGTG [CT]AC^GT[GA] 751 TACGTG BsaWI 575 ACCGGA [AT]^CCGG[AT] 975 TCCGGA BsmAI 85 GTCTC GTCTC(1/5) 505 GTCTC BsoBI 17 CCCGGG C^[CT]CG[GA]G 486 CTCGGG BspMI 263 ACCTGC ACCTGC(4/8) 671 ACCTGC BssHII 214 GCGCGC G^CGCGC 399 GCGCGC DpnII 534 GATC ^GATC 1180 GATC DsaI 257 CCACGG C^C[GA][CT]GG 676 CCGCGG HgaI 42 GCGTC GACGC(5/10) 503 GCGTC HinfI 990 GAATC G^A[ACGT]TC 1038 GATTC MaeI 61 CTAG C^TAG 778 CTAG MaeII 275 ACGT A^CGT 752 ACGT MaeIII 916 GTGAC ^GT[ACGT]AC 1031 GTGAC MboI 534 GATC ^GATC 1180 GATC NaeI 322 GCCGGC GCC^GGC 338 GCCGGC NdeII 534 GATC ^GATC 1180 GATC NgoMIV 322 GCCGGC G^CCGGC 338 GCCGGC PpuMI 812 AGGTCCT [GA]G^G[AT]CC[CT] 995 GGGTCCT PshAI 37 GACGGGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 466 GACCTCAGTC PstI 625 CTGCAG CTGCA^G 1173 CTGCAG Sau3AI 534 GATC ^GATC 1180 GATC SfcI 625 CTGCAG C^T[GA][CT]AG 1173 CTGCAG TfiI 990 GAATC G^A[AT]TC 1038 GATTC Tsp45I 916 GTGAC ^GT[GC]AC 1031 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 69 enzymes) # ######################################### AccII AciI AcyI AluI AspHI AvaII BanI BbeI BbvI BcnI BmyI BsaHI BsaJI BseRI BsiHKAI BsiYI BslI BsmFI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DraII EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HpaII HphI ItaI KasI MboII MnlI MseI MspA1I MspI MvaI MvnI MwoI NarI NciI NlaIII NlaIV Sau96I ScrFI SfaNI SfoI SmlI TaqI Tru9I TseI TspRI TthHB8I