(Circular) (Six-base) MAPSORT of: pFN21AE5593.seq Check: 6466 from: 1 to: 5965 pFN21AE5593 5965 bp With 182 enzymes: SgfI * January 30, 2014 17:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3775 278 Size: 53 83 186 936 2239 2468 Fragments arranged by size: 2468 2239 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2854 3308 1937 Size: 917 454 4594 Fragments arranged by size: 4594 917 454 Acc65I G'GTAC_C Cuts at: 3286 3286 Size: 5965 AclI AA'CG_TT Cuts at: 1563 4092 4465 1563 Size: 2529 373 3063 Fragments arranged by size: 3063 2529 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2583 4028 5412 5680 1632 Size: 951 1445 1384 268 1917 Fragments arranged by size: 1917 1445 1384 951 268 AfeI AGC'GCT Cuts at: 2070 2070 Size: 5965 AflII C'TTAA_G Cuts at: 820 1017 2725 820 Size: 197 1708 4060 Fragments arranged by size: 4060 1708 197 AflIII A'CryG_T Cuts at: 1203 2757 4864 5841 1203 Size: 1554 2107 977 1327 Fragments arranged by size: 2107 1554 1327 977 AgeI A'CCGG_T Cuts at: 4796 4796 Size: 5965 AhdI GACnn_n'nnGTC Cuts at: 4694 4694 Size: 5965 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5965 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5965 AlwNI CAG_nnn'CTG Cuts at: 3708 5280 3708 Size: 1572 4393 ApaI G_GGCC'C Cuts at: 2030 2030 Size: 5965 ApaLI G'TGCA_C Cuts at: 2625 4022 5178 2625 Size: 1397 1156 3412 Fragments arranged by size: 3412 1397 1156 ApoI r'AATT_y Cuts at: 1493 3274 3500 3652 5761 1493 Size: 1781 226 152 2109 1697 Fragments arranged by size: 2109 1781 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4519 160 Size: 872 3487 1606 Fragments arranged by size: 3487 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5965 AvaI C'yCGr_G Cuts at: 1870 1943 2022 2491 3290 1870 Size: 73 79 469 799 4545 Fragments arranged by size: 4545 799 469 79 73 BamHI G'GATC_C Cuts at: 3295 3662 3295 Size: 367 5598 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2137 2257 2330 2411 2715 Size: 325 217 627 350 120 73 81 304 Cuts at: 2715 2831 3286 4741 618 Size: 116 455 1455 1842 Fragments arranged by size: 1842 1455 627 455 350 325 304 217 120 116 81 73 BanII G_rGCy'C Cuts at: 1391 2030 2640 3284 1391 Size: 639 610 644 4072 Fragments arranged by size: 4072 644 639 610 BbeI G_GCGC'C Cuts at: 2141 2334 2415 2141 Size: 193 81 5691 Fragments arranged by size: 5691 193 81 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2091 3067 928 Size: 971 192 976 3826 Fragments arranged by size: 3826 976 971 192 BbvCI CC'TCA_GC Cuts at: 2378 2450 2378 Size: 72 5893 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4156 4190 1903 Size: 34 50 34 2135 34 3678 Fragments arranged by size: 3678 2135 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3857 5067 3857 Size: 1210 4755 BclI T'GATC_A Cuts at: 1548 1548 Size: 5965 BfrBI ATG'CAT Cuts at: 3160 3160 Size: 5965 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2615 4576 136 Size: 107 122 71 631 605 943 1961 1525 Fragments arranged by size: 1961 1525 943 631 605 122 107 71 BglII A'GATC_T Cuts at: 5960 5960 Size: 5965 BlpI GC'TnA_GC Cuts at: 3382 3382 Size: 5965 Bme1580I G_kGCm'C Cuts at: 1790 2030 2192 2262 2559 2629 4026 5182 1790 Size: 240 162 70 297 70 1397 1156 2573 Fragments arranged by size: 2573 1397 1156 297 240 162 70 70 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5965 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2514 3696 4654 Size: 416 53 83 399 24 1097 1182 958 Cuts at: 4654 442 Size: 1753 Fragments arranged by size: 1753 1182 1097 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5965 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2819 4625 1239 Size: 1580 1806 2579 Fragments arranged by size: 2579 1806 1580 Bpu10I CC'TnA_GC Cuts at: 2378 2450 2378 Size: 72 5893 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2798 2915 4090 4955 5253 2798 Size: 117 1175 865 298 3510 Fragments arranged by size: 3510 1175 865 298 117 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2095 4628 882 Size: 617 596 2533 2219 Fragments arranged by size: 2533 2219 617 596 BsaAI yAC'GTr Cuts at: 493 1204 2281 2758 3756 493 Size: 711 1077 477 998 2702 Fragments arranged by size: 2702 1077 998 711 477 BsaBI GATnn'nnATC Cuts at: 5959 5959 Size: 5965 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2047 2138 2331 2412 Size: 53 83 186 936 514 91 193 81 Cuts at: 2412 3772 4154 4789 275 Size: 1360 382 635 1451 Fragments arranged by size: 1451 1360 936 635 514 382 193 186 91 83 81 53 BsaWI w'CCGG_w Cuts at: 2580 2980 4398 4796 5070 5217 5628 2580 Size: 400 1418 398 274 147 411 2917 Fragments arranged by size: 2917 1418 411 400 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5740 Fragments arranged by size: 5740 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2083 2101 2119 2122 1186 Size: 897 18 18 3 5029 Fragments arranged by size: 5029 897 18 18 3 BseYI C'CCAG_C Cuts at: 2085 2166 2429 2705 5168 5664 2085 Size: 81 263 276 2463 496 2386 Fragments arranged by size: 2463 2386 496 276 263 81 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2969 1163 Size: 573 1233 4159 Fragments arranged by size: 4159 1233 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2348 3415 4176 5204 1540 Size: 275 186 347 1067 761 1028 2301 Fragments arranged by size: 2301 1067 1028 761 347 275 186 BsiHKAI G_wGCw'C Cuts at: 2021 2210 2322 2629 4026 4111 5182 2021 Size: 189 112 307 1397 85 1071 2804 Fragments arranged by size: 2804 1397 1071 307 189 112 85 BsmI GAATG_Cn' Cuts at: 3476 3569 3476 Size: 93 5872 BsmBI CGTCTCn'nnnn_ Cuts at: 2515 2515 Size: 5965 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2021 2030 2192 2210 2262 2322 2559 Size: 399 231 9 162 18 52 60 237 Cuts at: 2559 2629 2640 3284 4026 4111 5182 1391 Size: 70 11 644 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 644 399 237 231 162 85 70 60 52 18 11 9 BspEI T'CCGG_A Cuts at: 2980 5628 2980 Size: 2648 3317 BspHI T'CATG_A Cuts at: 3854 5584 3854 Size: 1730 4235 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2277 2685 3320 844 Size: 1433 408 635 3489 Fragments arranged by size: 3489 1433 635 408 BsrBI CCG'CTC Cuts at: 1394 1823 2572 3852 1394 Size: 429 749 1280 3507 Fragments arranged by size: 3507 1280 749 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2506 4460 4634 65 Size: 1183 1258 1954 174 1396 Fragments arranged by size: 1954 1396 1258 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2300 2327 2343 2961 4609 4796 1700 Size: 110 490 27 16 618 1648 187 2869 Fragments arranged by size: 2869 1648 618 490 187 110 27 16 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5965 BssHII G'CGCG_C Cuts at: 1925 2219 2404 1925 Size: 294 185 5486 Fragments arranged by size: 5486 294 185 BssSI C'ACGA_G Cuts at: 4025 5037 4025 Size: 1012 4953 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5965 BstBI TT'CG_AA Cuts at: 4769 4769 Size: 5965 BstEII G'GTnAC_C Cuts at: 1184 2920 1184 Size: 1736 4229 BstYI r'GATC_y Cuts at: 1972 3295 3662 4047 4064 5516 5602 5614 5960 Size: 1323 367 385 17 1452 86 12 346 Cuts at: 5960 1972 Size: 1977 Fragments arranged by size: 1977 1452 1323 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2711 2711 Size: 5965 BtgI C'CryG_G Cuts at: 513 1065 2262 2681 513 Size: 552 1197 419 3797 Fragments arranged by size: 3797 1197 552 419 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5226 Fragments arranged by size: 5226 683 56 BtsI GCAGTG_nn' Cuts at: 2627 3481 4275 4295 2627 Size: 854 794 20 4297 Fragments arranged by size: 4297 854 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5965 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5930 DraI TTT'AAA Cuts at: 3269 3269 Size: 5965 DraIII CAC_nnn'GTG Cuts at: 2554 2554 Size: 5965 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2758 4972 809 Size: 507 1442 2214 1802 Fragments arranged by size: 2214 1802 1442 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2313 2345 2364 Size: 54 1425 50 275 18 483 32 19 Cuts at: 2364 2466 2655 3412 4301 5930 8 Size: 102 189 757 889 1629 43 Fragments arranged by size: 1629 1425 889 757 483 275 189 102 54 50 43 32 19 18 EagI C'GGCC_G Cuts at: 1537 1812 2345 3412 1537 Size: 275 533 1067 4090 Fragments arranged by size: 4090 1067 533 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2297 3895 1353 Size: 944 1598 3423 Fragments arranged by size: 3423 1598 944 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2143 2152 2372 2630 4552 4926 Size: 1064 138 554 9 220 258 1922 374 Cuts at: 4926 5072 387 Size: 146 1280 Fragments arranged by size: 1922 1280 1064 554 374 258 220 146 138 9 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2583 2819 4028 4625 5412 5680 1239 Size: 393 951 236 1209 597 787 268 1524 Fragments arranged by size: 1524 1209 951 787 597 393 268 236 EcoNI CCTnn'n_nnAGG Cuts at: 2396 2396 Size: 5965 EcoO109I rG'GnC_Cy Cuts at: 2026 2027 2818 3001 2026 Size: 1 791 183 4990 Fragments arranged by size: 4990 791 183 1 EcoRI G'AATT_C Cuts at: 3274 3652 3274 Size: 378 5587 FspI TGC'GCA Cuts at: 2285 4471 2285 Size: 2186 3779 HaeII r_GCGC'y Cuts at: 1678 2072 2141 2334 2415 3108 5112 1678 Size: 394 69 193 81 693 2004 2531 Fragments arranged by size: 2531 2004 693 394 193 81 69 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3201 3233 4606 4638 4680 Size: 32 135 32 1629 32 1373 32 42 Cuts at: 4680 4712 1373 Size: 32 2626 Fragments arranged by size: 2626 1629 1373 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2239 3309 3555 669 Size: 988 281 301 1070 246 3079 Fragments arranged by size: 3079 1070 988 301 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5965 HpaI GTT'AAC Cuts at: 2239 3555 2239 Size: 1316 4649 KasI G'GCGC_C Cuts at: 2137 2330 2411 2137 Size: 193 81 5691 Fragments arranged by size: 5691 193 81 KpnI G_GTAC'C Cuts at: 3290 3290 Size: 5965 MfeI C'AATT_G Cuts at: 3564 3564 Size: 5965 MluI A'CGCG_T Cuts at: 5841 5841 Size: 5965 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5079 5263 1305 Size: 3774 184 2007 Fragments arranged by size: 3774 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2657 10 Size: 54 1425 343 825 3318 Fragments arranged by size: 3318 1425 825 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2600 3923 4282 4441 518 Size: 648 1434 1323 359 159 2042 Fragments arranged by size: 2042 1434 1323 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2176 2311 2353 2382 2683 3381 4058 5206 Size: 570 135 42 29 301 698 677 1148 Cuts at: 5206 5451 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 698 677 570 301 245 135 42 29 NaeI GCC'GGC Cuts at: 2329 2345 2329 Size: 16 5949 NarI GG'CG_CC Cuts at: 2138 2331 2412 2138 Size: 193 81 5691 Fragments arranged by size: 5691 193 81 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5413 NdeI CA'TA_TG Cuts at: 387 387 Size: 5965 NgoMIV G'CCGG_C Cuts at: 2327 2343 2327 Size: 16 5949 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5965 NotI GC'GGCC_GC Cuts at: 3412 3412 Size: 5965 NsiI A_TGCA'T Cuts at: 3162 3162 Size: 5965 NspI r_CATG'y Cuts at: 1132 1229 3323 4868 1132 Size: 97 2094 1545 2229 Fragments arranged by size: 2229 2094 1545 97 PasI CC'CwG_GG Cuts at: 1348 2889 1348 Size: 1541 4424 PciI A'CATG_T Cuts at: 4864 4864 Size: 5965 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5965 PfoI T'CCnGG_A Cuts at: 2874 2874 Size: 5965 PmeI GTTT'AAAC Cuts at: 3269 3269 Size: 5965 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4034 4066 5547 5579 4034 Size: 32 1481 32 4420 Fragments arranged by size: 4420 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2818 3001 2818 Size: 183 5782 PshAI GACnn'nnGTC Cuts at: 1613 2046 2475 1613 Size: 433 429 5103 Fragments arranged by size: 5103 433 429 PsiI TTA'TAA Cuts at: 3535 3535 Size: 5965 PspOMI G'GGCC_C Cuts at: 2026 2026 Size: 5965 PstI C_TGCA'G Cuts at: 830 2634 3182 3317 830 Size: 1804 548 135 3478 Fragments arranged by size: 3478 1804 548 135 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5965 PvuII CAG'CTG Cuts at: 2311 2311 Size: 5965 SacII CC_GC'GG Cuts at: 2684 2684 Size: 5965 SalI G'TCGA_C Cuts at: 1936 3307 1936 Size: 1371 4594 SbfI CC_TGCA'GG Cuts at: 3182 3317 3182 Size: 135 5830 ScaI AGT'ACT Cuts at: 4213 5925 4213 Size: 1712 4253 SfcI C'TryA_G Cuts at: 826 1045 2630 3178 3313 4448 5129 5320 826 Size: 219 1585 548 135 1135 681 191 1471 Fragments arranged by size: 1585 1471 1135 681 548 219 191 135 SfoI GGC'GCC Cuts at: 2139 2332 2413 2139 Size: 193 81 5691 Fragments arranged by size: 5691 193 81 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5965 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5965 SmaI CCC'GGG Cuts at: 1872 2024 3292 1872 Size: 152 1268 4545 Fragments arranged by size: 4545 1268 152 SmlI C'TyrA_G Cuts at: 820 1017 1943 2725 2813 2930 4069 4970 5232 Size: 197 926 782 88 117 1139 901 262 Cuts at: 5232 820 Size: 1553 Fragments arranged by size: 1553 1139 926 901 782 262 197 117 88 SnaBI TAC'GTA Cuts at: 493 3756 493 Size: 3263 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5965 SphI G_CATG'C Cuts at: 1132 3323 1132 Size: 2191 3774 SspI AAT'ATT Cuts at: 5 52 3889 5 Size: 47 3837 2081 Fragments arranged by size: 3837 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5965 StyI C'CwwG_G Cuts at: 513 1065 3404 513 Size: 552 2339 3074 Fragments arranged by size: 3074 2339 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2267 3992 4009 4162 4347 4792 325 Size: 1255 687 1725 17 153 185 445 1498 Fragments arranged by size: 1725 1498 1255 687 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4211 5923 96 Size: 274 80 33 51 1443 2234 1712 138 Fragments arranged by size: 2234 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5925 5957 5925 Size: 32 5933 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5965 XbaI T'CTAG_A Cuts at: 2782 3301 2782 Size: 519 5446 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2527 2527 Size: 5965 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5965 XmaI C'CCGG_G Cuts at: 1870 2022 3290 1870 Size: 152 1268 4545 Fragments arranged by size: 4545 1268 152 XmnI GAAnn'nnTTC Cuts at: 4094 4094 Size: 5965 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3773 276 Size: 53 83 186 936 2239 2468 Fragments arranged by size: 2468 2239 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BplI BsiWI BstXI BstZ17I EcoICRI EcoRV FalI FseI FspAI NruI PacI PmlI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI